Anatomical and hormonal factors determining the development of haploid and zygotic embryos of oat (Avena sativa L.)

Dziurka, Kinga; Dziurka, Michał; Muszyńska, Ewa; Czyczyło-Mysza, Ilona; Warchoł, Marzena; Juzoń, Katarzyna; Laskoś, Kamila; Skrzypek, Edyta

doi:10.1038/s41598-021-04522-y

Cited by 16 publications

(12 citation statements)

References 54 publications

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“…Ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used for the analysis of phytohormones, according to Hura et al [110] and Dziurka et al [111]. A stable isotope-labeled internal standard mixture was added to lyophilized bacteria samples of about 20 mg.…”

Section: Profiling Of Plant Hormonesmentioning

confidence: 99%

Seeds of Stevia rebaudiana Bertoni as a Source of Plant Growth-Promoting Endophytic Bacteria with the Potential to Synthesize Rebaudioside A

Simlat

Ptak

Jaglarz

et al. 2023

IJMS

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In this study, a new strain of Pantoea vagans, SRS89, was isolated from surface-sterilized stevia seeds. The isolate was evaluated using morphological, molecular, and biochemical methods. The bacterium was 1.5 μm long, yellowish in color, and classified as Gram-negative. Whole genome sequencing of our strain revealed the presence of a 4,610,019 bp chromosome, and genome annotation resulted in the detection of 4283 genes encoding 4204 putative coding sequences. Phylogenic analysis classified the genome of our strain close to the MP7 and LMG 24199 strains of P. vagans. Functional analysis showed that the highest number of genes within the analyzed bacterium genome were involved in transcription, amino acid transport and metabolism, and carbohydrate transport and metabolism. We also identified genes for enzymes involved in the biosynthesis of carotenoids and terpenoids. Furthermore, we showed the presence of growth regulators, with the highest amount noted for gibberellic acid A3, indole-3-acetic acid, and benzoic acid. However, the most promising property of this strain is its ability to synthesize rebaudioside A; the estimated amount quantified using reversed-phase (RP)-HPLC was 4.39 mg/g of the dry weight of the bacteria culture. The isolated endophytic bacterium may be an interesting new approach to the production of this valuable metabolite.

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Section: Profiling Of Plant Hormonesmentioning

confidence: 99%

Seeds of Stevia rebaudiana Bertoni as a Source of Plant Growth-Promoting Endophytic Bacteria with the Potential to Synthesize Rebaudioside A

Simlat

Ptak

Jaglarz

et al. 2023

IJMS

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“…Гаплоиндукторы кукурузы успешно использованы для получения гаплоидных растений пшеницы (Laurie, Bennett, 1988) и овса (Dziurka et al, 2022). Исследователи ведут интенсивный поиск других возможностей использования гаплоиндукторов кукурузы в селекции растений.…”

Section: кукуруза Zea Maysunclassified

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Курина

Artemyeva

2023

Biotehnologiâ i selekciâ rastenij

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Basic and applied scientific research in plant cell technologies contribute to the successful development of agricultural plant breeding, which allows the creation of new forms of plants 2-4 times faster than by traditional breeding methods. To obtain inbred lines of most vegetable crops, about 5-7 cycles of self-pollination are required. As a result, the creation of a new cultivar/hybrid takes more than 10-12 years on an average. To successfully create a variety or hybrid, it is necessary to select parental pairs in the form of inbred lines. The VIR collection of vegetables and cucurbit crops includes 52,889 accessions, representatives of 29 families, 145 genera, and 610 species. The use of biotechnological methods is an important direction for accelerating the breeding of vegetable crops. Due to the relevance of introducing cell technologies into the breeding programs of the VIR Department of Genetic Resources of Vegetable and Cucurbit Crops, a Laboratory of Breeding and Cell Technologies was set up in 2022. The goal of the research to be performed at the new laboratory is to accelerate the creation of source material, cultivars and hybrids by combining traditional breeding methods and cell technologies. The objects of the study include cultivated forms and wild relatives of cabbage Brassica oleracea L., turnip Brassica rapa L., lettuce Lactuca L., tomato Lycopersicon Mill and vegetable sweet corn Zea mays var. saccharata Sturt. In the present review, we consider the main results of breeding cabbage, tomato, and lettuce which have been obtained through applying cell technologies. Despite the progress obtained, there are still several problems in this area. The lack of standardized, efficient and reproducible protocols for in vitro methods often hinders their practical use. The tasks facing the laboratory in creating the initial breeding material and new cultivars and hybrids with the use of both conventional methods and cell technologies are relevant and correspond to the world level.

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“…Analyses of plant hormones were performed according to the methods reported previously [27,35,[54][55][56]. At 6 to 7 days after treatment with JA-Me, the below (nonsenescent, green) and above (senescent, yellow) parts of ca.…”

Section: Analyses Of Plant Hormones In Relation To the Formation Of The Secondary Abscission Zone Induced By Methyl Jasmonatementioning

confidence: 99%

A Possible Mode of Action of Methyl Jasmonate to Induce the Secondary Abscission Zone in Stems of Bryophyllum calycinum: Relevance to Plant Hormone Dynamics

Dziurka

Góraj-Koniarska

Marasek-Ciołakowska

et al. 2022

Plants

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Plants can react to environmental stresses through the abscission of infected, damaged, or senescent organs. A possible mode of action of methyl jasmonate (JA-Me) to induce the formation of the secondary abscission zone (SAZ) in the stems of Bryophyllum calycinum was investigated concerning plant hormone dynamics. Internode segments were prepared mainly from the second or third internode from the top of plants with active elongation. JA-Me applied to the middle of internode segments induced the SAZ formation above and below the treatment after 5–7 days. At 6 to 7 days after JA-Me treatment, the above and below internode pieces adjacent to the SAZ were excised and subjected to comprehensive analyses of plant hormones. The endogenous levels of auxin-related compounds between both sides adjacent to the SAZ were quite different. No differences were observed in the level of jasmonic acid (JA), but the contents of 12-oxo-phytodienoic acid (OPDA), a precursor of JA, and N-jasmonyl-leucine (JA-Leu) substantially decreased on the JA-Me side. Almost no effects of JA-Me on the dynamics of other plant hormones (cytokinins, abscisic acid, and gibberellins) were observed. Similar JA-Me effects on plant hormones and morphology were observed in the last internode of the decapitated growing plants. These suggest that the application of JA-Me induces the SAZ in the internode of B. calycinum by affecting endogenous levels of auxin- and jasmonate-related compounds.

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Anatomical and hormonal factors determining the development of haploid and zygotic embryos of oat (Avena sativa L.)

Cited by 16 publications

References 54 publications

Seeds of Stevia rebaudiana Bertoni as a Source of Plant Growth-Promoting Endophytic Bacteria with the Potential to Synthesize Rebaudioside A

Seeds of Stevia rebaudiana Bertoni as a Source of Plant Growth-Promoting Endophytic Bacteria with the Potential to Synthesize Rebaudioside A

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

A Possible Mode of Action of Methyl Jasmonate to Induce the Secondary Abscission Zone in Stems of Bryophyllum calycinum: Relevance to Plant Hormone Dynamics

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