Anatilimnocola floriformis sp. nov., a novel member of the family Pirellulaceae from a boreal lake, and emended description of the genus Anatilimnocola

Abstract

we characterized a novel freshwater planctomycete of the genus 'Anatilimnocola', strain PX40 T , which was isolated from a boreal eutrophic lake. Strain PX40 T was represented by budding, unpigmented, ellipsoidal to pear-shaped cells, which often occurred in characteristic flower-like rosettes. Cells were covered by bundles of fimbriae; crateriform-like structures were localized on a reproductive cell pole only. These planctomycetes were obligately aerobic, heterotrophic bacteria that utilized various sugars and some polysaccharides, and were highly sensitive to NaCl. Growth occurred in the pH range 5.0-7.5 (with an optimum at pH 6.5-7.0), and at temperatures between 15 and 30 °C (with an optimum at 22-25 °C). The major fatty acids of strain PX40 T were C18:1ω9c, C16:0, and 16:1ω7c; cells also contained a wide variety of hydroxy-and dihydroxy-fatty acids and a C31:9 alkene. The major intact polar lipids were diacylglyceryl-(N,N,N)-trimethylhomoserines. The 16S rRNA gene sequence of strain PX40 T displayed 96.6% similarity to that of 'Anatilimnocola aggregata' ETA_A8 T . The genome of strain PX40 T was 8.93 Mb in size and contained one copy of rRNA operon, 76 tRNA genes and 7092 potential proteincoding genes. The DNA G+C content was 57.8%. The ANI value between strain PX40 T and 'Anatilimnocola aggregata' ETA_A8 T was 78.3%, suggesting that these planctomycetes represent distinct species. We, therefore, propose a novel species of the genus 'Anatilimnocola', 'A. floriformis' sp. nov., with strain

Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments

“…A phylogenetic tree was constructed to summarize the ecology of ICT_E10.1 T -related Planctomycetota and other strains closely related to the genus Stieleria (Figure 3). In summary, strain ICT_E10.1 T and its closest uncultured relatives appear to be associated with brackish and marine ecosystems and therefore adapted to tolerate salt, as well as the other members of the genus Stieleria and most Pirellulaceae (only two genera in the family are currently associated with freshwater environments: Pirellula and "Anatilimnocola" [18,47,89,90]).…”

Co-exposure of dimethomorph and imidacloprid: effects on soil bacterial communities in vineyard soil

“…As for the family Pirellulaceae, they can generally be found in fresh and marine water environments (Kulichevskaya et al, 2022). They were reported to be an ammonia-oxidizing bacteria, thus participating in the nitrogen cycle (de Celis et al, 2020).…”

Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

“…The vast majority of estimated bacterial diversity is still uncultured in laboratory (Barer and Harwood 1999 ; Rinke, et al 2013 ), which heavily restricts what could be screened for biotechnological purposes. Nonetheless, recent cultivation efforts from diverse research groups have allowed to bring into axenic culture many strains (including novel species) from underexplored and evolutionarily deep-branching groups such as the Planctomycetota (Almeida, et al 2022 , Dedysh, et al 2020 , Gaurav, et al 2021 , Kaushik, et al 2020 , Kulichevskaya, et al 2020a , Kulichevskaya, et al 2022 , Kulichevskaya, et al 2020b , Kumar, et al 2020a , Kumar, et al 2020b , Kumar, et al 2021a , 2021b, Vitorino, et al 2020 , Vitorino et al 2021b , Vitorino, et al 2022b , Vitorino, et al 2022c , Vitorino, et al 2022d , Wiegand, et al 2020 ). The bacterial phylum Planctomycetes (now renamed Planctomycetota (Oren and Garrity 2021 )) belongs to the super phylum Planctomycetota - Chlamydiota - Verrucomicrobiota (PVC) (Rivas-Marín and Devos 2018 ; Wagner and Horn 2006 ).…”