Anastomosis Groups among Isolates of Ceratobasidium cornigerum and Related Fungi

Burpee, L. L.; Sanders, P. L.; Cole, H.; Sherwood, R. T.

doi:10.2307/3759762

Cited by 84 publications

(41 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rhizoctonia cerealis, a basidiomycete belonging to the subdivision Deuteromycotina, generates no asexual spores and lives in soil in the form of sclerotia and mycelia. According to the results of a hyphal fusion test, R. cerealis belongs to the AG-D group (Burpee et al 1980;Ogoshi 1987).…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR

Guo

Sun

et al. 2012

J Gen Plant Pathol

View full text Add to dashboard Cite

Rhizoctonia cerealis E.P. van der Hoeven (anamorph of Ceratobasidium cereale D.I. Murray and Burpee), which causes sharp eyespot in wheat, is a major soilborne fungal pathogen that severely impairs yield and quality of winter wheat in China. Because the pathogen is difficult to identify and quantify in soil using conventional methods, a rapid and reliable method is needed to detect and quantify the fungus. In this study, we developed an SYBR Green-based quantitative real-time polymerase chain reaction assay for specific, sensitive detection and quantification of R. cerealis in soil samples. Using a specific primer pair based on the b-tubulin gene of the fungal DNA sequence, we could specifically detect R. cerealis at quantities as low as 100 fg of purified pathogen DNA. Using the real-time PCR assays, we were able to quantify R. cerealis in artificially and naturally infested soil samples. This new technique to quantify R. cerealis is rapid and accurate and will be a useful tool for future studies of pathogenic R. cerealis.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR

Guo

Sun

et al. 2012

J Gen Plant Pathol

View full text Add to dashboard Cite

show abstract

“…The fi rst part also included a more detailed multiple alignment of rDNA-internal transcribed spacer (ITS) sequence analysis of the multinucleate (MNR) anastomosis groups (AGs) and subgroups of R. solani, R. praticola, and R. circinata (teleomorphs: Thanatephorus cucumeris, T. praticola, and Waitea circinata, respectively; Sharon et al 2006). Burpee et al (1980) divided the binucleate Rhizoctonia (BNR) (Ceratobasidium cornigerum and related fungi) into 7 "Ceratobasidium Anastomosis Groups" (CAG-1 to CAG-7), whereas Ogoshi et al (1983) divided the BNR into 17 anastomosis groups (AG-A to AG-Q). As the isolates of most of the different CAGs were the same as some of the BNR AGs designated in Japan (Ogoshi et al 1983), they were subsequently incorporated into the AG system developed in Japan.…”

Section: Introductionmentioning

confidence: 99%

Classification of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping

et al. 2008

View full text Add to dashboard Cite

Classifi cation of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping Abstract Currently, rDNA-ITS sequence analysis seems to be the most appropriate method for comprehensive classifi cation of Rhizoctonia spp. Our previous review article was concerned with detailed analysis of multinucleate Rhizoctonia (MNR), and the current review complements the previous one with detailed analysis of binucleate Rhizoctonia (BNR) (teleomorphs: Ceratobasidium spp. and Tulasnella spp.) and uninucleate Rhizoctonia (UNR) (teleomorph: C. bicorne). Data of all the appropriate BNR and UNR accumulated in GenBank were analyzed together in neighborjoining (NJ) trees supplemented with percent sequence similarity within and among the anastomosis groups (AGs) and subgroups. Generally, the clusters of the isolate sequences supported the genetic basis for the AG based on hyphal fusion anastomosis. Comprehensive interrelationships among all the currently available MNR, BNR, and UNR groups and subgroups in GenBank were subsequently analyzed in NJ and maximum-parsimony (MP) trees, showing the genetic relatedness among the different groups and indicating possible bridging groups between MNR, BNR, and UNR. The review also indicates serious inaccuracies in designation of sequences of some isolates deposited in GenBank. Several additional teleomorph genera with Rhizoctonia spp. anamorphs have also been reported in the literature. However, as they have not been intensively studied, there were no available data on their rDNA-ITS sequences that could be included in this review.

show abstract

“…Plant pathogenic species of Rhizoctonia are widely distributed in the world and consist of large and complex groups in fungi (Burpee et al 1980;Ogoshi 1987). Rhizoctonia solani Kühn (teleomorph Thanatephorus cucumeris (Frank) Donk), a soil-borne fungus with peculiar versatility, has been considered as a species complex because of its many intraspecific variations in pathogenicity, host range, and physiological and morphological characteristics (Adams 1988).…”

mentioning

confidence: 99%

Trials of direct detection and identification of Rhizoctonia solani AG 1 and AG 2 subgroups using specifically primed PCR analysis

Matsumoto

2002

Mycoscience

View full text Add to dashboard Cite

Specifically primed polymerase chain reaction (PCR) analysis was used for direct detection and identification of Rhizoctonia solani isolates belonging to AG 1 subgroups (IA, IB, and IC) and AG 2 subgroups (2-1 and 2-2). A rapid DNA extraction method with a solution of sodium hydroxide was conducted to extract PCR templates. PCRspecific primer sets for each group were designed from sequences in the regions of the 28S ribosomal DNA of this fungus. The results of specifically primed PCR analysis showed that AG 1-IA, AG 1-IB, AG 1-IC, AG 2-1, and AG 2-2 primers sets contributed detection from the same AG isolates and could escape detection from different AG isolates at a high level of frequency. In this experiment, we suggested that our synthesized primer sets might provide a method for the direct detection and identification of AGs of R. solani.

show abstract

Anastomosis Groups among Isolates of Ceratobasidium cornigerum and Related Fungi

Cited by 84 publications

References 16 publications

Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR

Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR

Classification of Rhizoctonia spp. using rDNA-ITS sequence analysis supports the genetic basis of the classical anastomosis grouping

Trials of direct detection and identification of Rhizoctonia solani AG 1 and AG 2 subgroups using specifically primed PCR analysis

Contact Info

Product

Resources

About