Anaplastic Large Cell Lymphoma with Oral Manifestation: A Series of Four Cases and Literature Review

Mature T/NK‐Cell lymphomas of the oral and maxillofacial region: A multi‐institutional collaborative study

“…Accordingly, the phenotypic profile of tumour cells in ALK + ALCL includes CD30+, while 60%–70% of ALK + cases are positive for EMA protein. Nearly 80% of ALK + cases are also positive for TIA1, granzyme B and perforin 9 …”

“…6 As demonstrated in our case, ALK + ALCL frequently occurs in extranodal sites, including skin, bone and soft tissue. ALCL involving the oral cavity has also been reported 9 and we recently documented four cases of ALCL (two ALK-positive and two ALK-negative cases), two of which affected the alveolar ridge; one in the submandibular region and floor of the mouth; and one in the hard palate. 9 Unlike individuals with the neoplasm affecting the skin and other organs, the mean age of patients with ALK + ALCL involving the oral cavity is 53.7 years and women are more affected (1.8:1).…”

“…ALCL involving the oral cavity has also been reported 9 and we recently documented four cases of ALCL (two ALK-positive and two ALK-negative cases), two of which affected the alveolar ridge; one in the submandibular region and floor of the mouth; and one in the hard palate. 9 Unlike individuals with the neoplasm affecting the skin and other organs, the mean age of patients with ALK + ALCL involving the oral cavity is 53.7 years and women are more affected (1.8:1). Accordingly, the phenotypic profile of tumour cells in ALK + ALCL includes CD30+, while 60%-70% of ALK + cases are positive for EMA protein.…”

“…Immunohistochemistry was performed in all cases using the streptavidin‐biotin peroxidase complex technique on formalin‐fixed paraffin‐embedded tissue. A large panel of immunohistochemical markers for the demonstration of T/NK‐cell differentiation, proliferative index and cytotoxic granules were variably employed for each case depending on the diagnostic work‐up for each subtype and followed previously published methods 9,10 . In situ hybridization (ISH) for the detection of Epstein‐Barr virus (EBV) was performed whenever recommended using a fluorescein‐labelled peptide nucleic acid probe (PNA) complementary to 2 nuclear‐encoded RNAs (EBER) (Y5200, Dako).…”

“…A large panel of immunohistochemical markers for the demonstration of T/NK-cell differentiation, proliferative index and cytotoxic granules were variably employed for each case depending on the diagnostic work-up for each subtype and followed previously published methods. 9,10 In situ hybridization (ISH) for the detection of Epstein-Barr virus (EBV) was performed whenever recommended using a fluorescein-labelled peptide nucleic acid probe (PNA) complementary to 2 nuclear-encoded RNAs (EBER) (Y5200, Dako). Methods: Cases diagnosed as mature T/NK-cell lymphomas affecting the oral and maxillofacial region were retrospectively retrieved from six pathology files and their diagnoses were confirmed using haematoxylin and eosin-stained slides, immunohistochemical reactions and in situ hybridization for Epstein-Barr virus (EBV) detection.…”