Anaplasma phagocytophilum, Bartonella spp., haemoplasma species and Hepatozoon spp. in ticks infesting cats: a large-scale survey

Duplan, Florent; Davies, Saran; Filler, Serina; Abdullah, Swaid; Keyte, Sophie; Newbury, Hannah; Helps, Christopher R; Wall, Richard; Tasker, Séverine

doi:10.1186/s13071-018-2789-5

Cited by 43 publications

(45 citation statements)

References 23 publications

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“…Ticks, mites, sand flies, tsetse flies, mosquitoes, fleas, lice, reduviid bugs and leeches were shown to serve as definitive hosts of different Hepatozoon species [3,4]. H. felis DNA was detected in four Rhipicephalus sanguineus ticks collected from two cats and one dog in Portugal [38]; in one Ixodes hexagonus tick from a cat in Wales (with only 90% sequence identity to available GenBank sequences) [39] and in 1.9% of 685 Ctenocephalides felis fleas collected from 185 cats in Israel [40], suggesting the possible involvement of these ectoparasites in the life cycle; however, no H. felis oocysts were detected in any arthropod vector so far. Alternative ways of transmission such as predation on an infected intermediate or paratenic host were described for H. americanum in dogs in US [5], but it is not known if this way of transmission may also occur or have any epidemiological relevance in Hepatozoon species affecting cats.…”

Section: Discussionmentioning

confidence: 99%

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

Basso

Görner²,

Globokar

et al. 2019

Parasitology International

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A B S T R A C TThree different Hepatozoon (Apicomplexa, Hepatozoidae) species have been described infecting domestic cats in Europe (i.e. H. felis, H. canis and H. silvestris), however, reports on clinical hepatozoonosis are uncommon and treatment protocols are not clearly defined.A six-year-old male European short-hair cat from Austria presented poor general condition, lethargy, anorexia, icterus, a painful abdomen, fever, ruffled hair and a tick infestation, and it had never left Austria. Laboratory tests revealed leukopenia, thrombocytopenia and increased serum levels of symmetric dimethylarginine (SDMA) and bilirubin. In May Grünwald-Giemsa-stained blood smears, structures resembling Hepatozoon gamonts were observed inside neutrophil granulocytes. A PCR targeting a fragment of the 18S rRNA gene of Hepatozoon spp. and DNA sequencing allowed the diagnosis of H. felis-DNA in blood samples. The cat was treated with imidocarb dipropionate (6 mg/kg body weight, repeated after 14 days) and doxycycline monohydrate (5 mg/kg body weight twice a day, p.o., for four weeks) and recovered completely. A broad haematological and biochemical laboratory control after six months showed all evaluated parameters under normal ranges. Coinfection with other feline pathogens (i.e. feline leukaemia virus, feline immunodeficiency virus, feline Coronavirus, Leishmania and Dirofilaria immitis) could not be detected.This study reveals the presence of H. felis in Austria and provides more evidence on the geographical distribution and pathogenicity of this parasite for domestic cats. To the authors' knowledge, this is the first autochthonous case of feline hepatozoonosis in Central Europe.

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Section: Discussionmentioning

confidence: 99%

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

Basso

Görner²,

Globokar

et al. 2019

Parasitology International

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“…Багато таких захворювань є зоонозами і призводять до інвалідності та смертності людей і тварин (Harrus & Baneth, 2005). У країнах ЄС було проведено багато досліджень щодо вивчення поширення іксодових кліщів і патогенів, які вони можуть передавати (Bonnet et al, 2013;Reye et al, 2013;Elsheikha, 2016;Kybicova et al, 2017;Roczeń-Karczmarz et al, 2018;Duplan et al, 2018). Щодо України, то нещодавно почали з'являтись повідомлення стосовно поширеності даних патогенів на території нашої держави (Karbowiak et al, 2013;Didyk et al, 2016;Rogovskyy et al, 2017;Weiner et al, 2018).…”

Section: вступunclassified

Monitoring of vector-borne diseases in the west part of Ukraine

Levytska

Mushinsky

2019

SMLNUVMB

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During recent years, infectious and invasive animal diseases caused by viruses, bacteria, and protozoa and transmitted by ticks have been a new problem in medical and veterinary practice. Many of these diseases are zoonoses and lead to the disability and mortality of humans and animals. Ixodes ricinus and Dermacentor reticulatus are species of ticks that are commonly attack animals and humans in Europe. These ticks are spread throughout the EU and are involved in the transmission of a large number of vector-borne diseases. To date, relatively limited data on the circulation of tick-borne diseases and their transmission in western Ukraine are presented. The purpose of this study was to monitor the types of ixodid ticks, as well as to identify pathogens in ticks collected from the environment in urban parks and from dogs and cats. A total of 215 ticks (22 Ixodes ricinus and 193 Dermacentor reticulatus) collected in two regions of Lviv and Ivano-Frankivsk were investigated. Pathogens, namely, Rikettsia spp., Candidatus Neoehrlichia mikurensis, Anaplasma phagocytophilum and Bartonella spp., were detected in 64.0% of the I. ricinus ticks. Overall, 18.2% of I. ricinus ticks were positive for Rikettsia spp., 54.5% for Candidatus Neoehrlichia mikurensis, 9.1% for A. phagocytophilum and Bartonella spp. confirmed in 4.5% of the studied ticks. Mixed infestations were found in 5 samples of I. ricinus DNA, representing 22.7%. In addition, 74.6% of D. reticulatus ticks were infested with these pathogens. Overall, 30.0% of D. reticulatus ticks were positive for Rikettsia spp., Candidatus Neoehrlichia mikurensis was confirmed in 54.9%, A. phagocytophilum in 1.6%, and Bartonella spp. in 6.2% of the studied ticks. Mixed infestations were found in 35 D. reticulatus DNA samples, representing 18.1%. The prevalence of Rikettsia spp. in two regions of Lviv and Ivano-Frankivsk, among the studied ticks was 28.8%, Bartonella spp. – 6.0%, A. phagocytophilum – 2.3%, Candidatus Neoehrlichia mikurensis – 54.9%. Our data indicate the presence of pathogens in urban populations of ticks of I. ricinus and D. reticulatus in west part of Ukraine. Monitoring of tick-borne diseases is an important tool in the prevention and control of infections transmitted to humans and animals.

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“…Several country-wide studies have been made in Europe to assess ticks and TBPs presence and distribution in companion animals [15][16][17][18][19][20]. In Italy, several efforts have been made to evaluate the prevalence of circulating tick-borne pathogens in ticks collected from dogs [21,22], although limited to certain areas.…”

Section: Introductionmentioning

confidence: 99%

Tick-borne pathogens in Ixodidae ticks collected from privately-owned dogs in Italy: a country-wide molecular survey

Zanet

Battisti

Pepe

et al. 2020

Preprint

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Background Ticks and tick-borne diseases are increasingly recognized as a cause of disease in dogs worldwide. The epidemiology of ticks and tick-transmitted protozoa and bacteria has changed due to the spread of ticks to urban and peri-urban areas and the movement of infected animals, posing new risks for animals and humans. This countrywide study reports information on distribution and prevalence of pathogens in ticks collected from privately-owned dogs in Italy. We analyzed 2681 Ixodidae ticks, collected from 1454 pet dogs from Italy. Specific PCR protocols were used to detect i) Piroplasms of the genera Babesia and Theileria , ii) Gram-negative cocci of the family Anaplasmataceae and iii) Borrelia burgdorferi sensu lato. Sequencing of positive amplicons allowed for species identification. Results Babesia / Theileria spp. DNA was detected in 435 homogeneous tick-pools (Minimum Infection Rate (MIR) = 27.6%; 95% confidence interval (CI) = 25.4-29.8%) with higher prevalence in Ixodes ricinus and Rhipicephalus sanguneus group. The zoonotic B. venatorum was the most prevalent species (MIR = 7.5%; 95% CI = 6.3-9.0%). Anaplasma and Ehrlichia species were detected in 165 tick-pools (MIR = 10.5%; 95% CI = 9.3-11.8%) and specifically, A. phagocytophilum was identified with MIR = 5.1% (95% CI = 4.1-6.3%). Borrelia burgdorferi s.l. and B. afzelii were detected with MIR = 0.4% (95% CI = 0.2-0.8%) and MIR = 0.3% (95% CI 0.1-0.7%) respectively. Conclusions Zoonotic pathogens B. venatorum and A. phagocytophilum were the most frequently detected in ticks collected from privately-owned dogs which might be used as markers of pathogens presence and distribution.

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Anaplasma phagocytophilum, Bartonella spp., haemoplasma species and Hepatozoon spp. in ticks infesting cats: a large-scale survey

Cited by 43 publications

References 23 publications

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

First autochthonous case of clinical Hepatozoon felis infection in a domestic cat in Central Europe

Monitoring of vector-borne diseases in the west part of Ukraine

Tick-borne pathogens in Ixodidae ticks collected from privately-owned dogs in Italy: a country-wide molecular survey

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