Anaplasma marginale outer membrane protein vaccine candidates are conserved in North American and South African strains

Hove, Paidashe; Brayton, Kelly A.; Liebenberg, Junita; Pretorius, Ashley; Oosthuizen, Marinda C.; Noh, Susan M.; Collins, Nicola E.

doi:10.1016/j.ttbdis.2020.101444

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…This vaccine has been widely utilized in many regions of the world and is effective in preventing clinical disease after infection caused by field strains of A. marginale [ 4 , 73 , 154 ]. However, it has the limitations of offering only partial protection when challenged by diverse strains of A. marginale and is likely to introduce new strains of infection in regions where A. marginale is nonendemic; thus, it is not used in such countries as the United States [ 155 ]. Other vaccines that have been developed to prevent bovine anaplasmosis include inactivated, cultured or killed A. marginale vaccines [ 73 , 156 , 157 , 158 ].…”

Section: Anaplasmosis Control In Africamentioning

confidence: 99%

“…The study speculated that minor intraspecific differences should not influence the possible cross-protective ability of antibody-mediated and cellular immune responses against various A. marginale strains worldwide [ 56 ]. In South Africa, a study identified five recombinant A. marginale OMPs from strains of A. marginale in the country that were suggested to be interesting vaccine candidates for use in novel global vaccine cocktails against A. marginale [ 155 ].…”

Section: Anaplasmosis Control In Africamentioning

confidence: 99%

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Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Kolo

2023

Pathogens

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Anaplasma species, belonging to the family Anaplasmataceae in the order Rickettsiales, are obligate intracellular bacteria responsible for various tick-borne diseases of veterinary and human significance worldwide. With advancements in molecular techniques, seven formal species of Anaplasma and numerous unclassified species have been described. In Africa, several Anaplasma species and strains have been identified in different animals and tick species. This review aims to provide an overview of the current understanding of the molecular epidemiology and genetic diversity of classified and unclassified Anaplasma species detected in animals and ticks across Africa. The review also covers control measures that have been taken to prevent anaplasmosis transmission on the continent. This information is critical when developing anaplasmosis management and control programs in Africa.

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Section: Anaplasmosis Control In Africamentioning

confidence: 99%

Section: Anaplasmosis Control In Africamentioning

confidence: 99%

Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Kolo

2023

Pathogens

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“…Bovine anaplasmosis, a tick-borne disease caused by the mandatory intracellular pathogen Anaplasma marginale, is responsible for high morbidity and mortality in cattle (Guarnizo et al, 2020;Hove et al, 2020). It is transmitted mainly by ticks, mosquitoes, flies, and transplacentally (Atif et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Anaplasma marginale transplacental transmission in a calf: clinical, anatomopathological, and molecular reports

Da Silva,

Bastos,

Resende Junior

et al. 2023

PRW

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Bovine anaplasmosis is caused by obligate intracellular rickettsia Anaplasma marginale transmitted by ticks Rhipicephalus microplus, hematophagous flies, and/or iatrogenically. Transplacental transmission is possible but sub-reported. In this context, the present work aimed to report a case of a calf that was born with anemia, jaundice, and neurological problems. A calf blood smear showed intraerythrocytic inclusion of A. marginale. Accordingly, the animal was treated with oxytetracycline but died three days after the start of treatment. The necropsy revealed conjunctival and subcutaneous jaundice, splenic congestion and distension, and an intensely yellow liver were noted. Histopathology showed moderate, multifocal hepatocellular degeneration, diffuse and marked cholestasis, and histiocytic necrotizing encephalitis. A. marginale infection was confirmed by PCR, and real-time PCR (qPCR). qPCR analysis showed that 3.3% of the erythrocytes in the calf's blood sample were parasitized. Thus, here we report a case of transplacental transmission A. marginale in a newborn calf.

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“…Many studies have validated the use of OMPs as successful vaccine candidates in GNBs such as Legionella pneumophila, Mannheimia haemolytica, Aeromonas salmonicida, Anaplasma marginale, Bartonella henselae, Campylobacter jejuni , and Avian Pathogenic E.coli (APEC) (Ayalew et al, 2010; Diao et al, 2020; Hove et al, 2020; Moumène et al, 2015). A copious amount of information has been generated through proteomic analysis on different types of families of porins such as OmpA and OprD family in Pseudomonas aeruginosa (Chevalier et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In-silico design and assessment of OprD based multi-epitope vaccine against Acinetobacter baumannii

Khalid

Andleeb

2022

Preprint

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Gram-negative, opportunist pathogen Acinetobacter baumannii is notorious for causing a plethora of nosocomial infections predominantly respiratory diseases and blood-stream infections. Due to resistance development towards last-resort antibiotics, its treatment is becoming increasingly difficult. Despite numerous therapeutic developments, no vaccine is available against this ubiquitous pathogen. It is therefore apropos to formulate a rational vaccine plan to get rid of the super-bug. Considering the importance of Outer Membrane Porin D (OprD) as a potential vaccine candidate, we methodically combined the most persistent epitopes present in the A. baumannii strains with the help of different immunoinformatic approaches to envisage a systematic multi-epitope vaccine. The proposed vaccine contains highly immunogenic stretches of linear B-cells, cytotoxic T lymphocyte epitopes, and helper T lymphocyte epitopes of outer membrane porin OprD. The finalized epitopes proved to be significant as they are conserved in A. baumannii strains. The final 3D structure of the construct was projected, refined, and verified by employing several in silico approaches. Apt binding of the protein and adjuvant with the TLR4 suggested significantly high immunogenic potential of our designed vaccine. MD simulations showed highly stable composition of the protein. Immune simulations disclosed a prominent increase in the levels of the immune response. The proposed vaccine model is proposed to be thermostable, immunogenic, water-soluble, and non-allergenic. However, this study is purely computational and needs to be validated by follow-up wet laboratory studies to confirm the safety and immunogenicity of our multi-epitope vaccine.

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Anaplasma marginale outer membrane protein vaccine candidates are conserved in North American and South African strains

Cited by 4 publications

References 40 publications

Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Anaplasma marginale transplacental transmission in a calf: clinical, anatomopathological, and molecular reports

In-silico design and assessment of OprD based multi-epitope vaccine against Acinetobacter baumannii

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