Anaphylaxis after hamster bites – identification of a novel allergen

Lim, Dawn; Chan, Raymond; Wen, Hongmei; Bever, Hugo P.S. Van; Chua, Kaw Yan

doi:10.1111/j.1365-2222.2004.01992.x

Cited by 24 publications

(14 citation statements)

References 3 publications

(6 reference statements)

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“…The results confirmed that MSP is an allergen and that it lacks cross-reactivity with the allergens in the fur of Siberian and Roborovski hamsters. This also confirmed the previous reports of lack of cross-reactivity between allergens of Golden and Siberian hamsters [2,3,5,7]. However, we noted a strong IgE cross-reactivity between the fur allergens of the Siberian and Roborovski hamster.…”

Section: Discussionsupporting

confidence: 81%

“…The Golden hamster is the main species used in animal research experiments. A number of studies have reported bronchial asthma upon exposure to hamsters as well as the occurrence of anaphylactic reactions after hamster bites [2,3,4,5,6,7,8,9,10]. Most cases are related to Siberian hamsters; only 1 study reported the occurrence of symptoms with other hamster species, namely the Roborovski, Golden and European hamster [4].…”

Section: Introductionmentioning

confidence: 99%

“…It appears, however, that the allergens originating from these four species are not identical. Indeed, patients allergic to the Siberian hamster did not react to commercial skin-prick test (SPT) preparations made from the European or the Golden hamster [2,3,5,7,8,10]. …”

Section: Introductionmentioning

confidence: 99%

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Male-Specific Submaxillary Gland Protein, a Lipocalin Allergen of the Golden Hamster, Differs from the Lipocalin Allergens of Siberian and Roborovski Dwarf Hamsters

Hilger

Dubey

Lentz

et al. 2015

Int Arch Allergy Immunol

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Background: An increasing number of asthma cases upon exposure to hamsters and anaphylactic reactions following hamster bites are being reported, but the allergens responsible are still poorly characterized. In the Golden hamster, male-specific submaxillary gland protein (MSP), a lipocalin expressed in a sex- and tissue-specific manner in the submaxillary and lacrimal glands, is secreted in the saliva, tears and urine. The purpose of this study was to determine if MSP is an allergen, to identify IgE-reactive proteins of different hamster species and to analyse potential cross-reactivities. Methods: Fur extracts were prepared from four hamster species. Hamster-allergic patients were selected based on a history of positive IgE-test to hamster epithelium. The IgE-reactivity of patients' sera was investigated by means of immunoblot and ELISA. IgE-reactive proteins in fur extracts and the submaxillary gland were identified using anti-MSP antibodies, Edman sequencing or mass spectrometry. MSP was purified from Golden hamster and recombinant MSP was expressed in E. coli. Results: Four patients had IgE-antibodies against 20.5-kDa and 24-kDa proteins of Golden hamster fur extract, which were identified as MSP. IgE-reactive MSP-like proteins were detected in European hamster fur extract. Three patient sera showed IgE-reactive bands at 17-21 kDa in Siberian and Roborovski hamster fur extracts. These proteins were identified as two closely related lipocalins. Immunoblot inhibition experiments showed that they are cross-reactive and are different from MSP. Conclusion: MSP lipocalin of the Golden hamster was identified as an allergen, and it is different from the cross-reactive lipocalin allergens of Siberian and Roborovski hamsters. Our findings highlight the need for specific tools for the in vitro and in vivo diagnosis of allergy to different hamster species.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

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Male-Specific Submaxillary Gland Protein, a Lipocalin Allergen of the Golden Hamster, Differs from the Lipocalin Allergens of Siberian and Roborovski Dwarf Hamsters

Hilger

Dubey

Lentz

et al. 2015

Int Arch Allergy Immunol

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Section: Introductionmentioning

confidence: 99%

“…Two previous reports of anaphylaxis induced by SH bites have been published [1,2]. In one case, the responsible allergen from the hamster saliva was identified as a 21-kDa protein that showed partial cross-reactivity with Dermatophagoides pteronyssinus [2]. Bertó et al [3] described 2 IgE-binding proteins (molecular weights 18 and 32 kDa) in 5 of the 6 sera samples taken from patients with asthma who were monosensitized to SH.…”

Section: Introductionmentioning

confidence: 99%

An Odorant-Binding Protein as a New Allergen from Siberian Hamster <i>(Phodopus sungorus)</i>

Torres

Pastor-Vargas²,

Heras³

et al. 2011

Int Arch Allergy Immunol

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A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding bands of about 18, 21 and 23 kDa. When the patient’s serum was preincubated with rabbit, mouse and gerbil hair extracts, no inhibition of the 3 SH IgE-binding bands was demonstrated. Proteins extracted from the 3 bands were analyzed by N-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry, and peptides were sequenced. IgE-binding bands were identified as being an odorant-binding protein belonging to the lipocalin family. Analysis of the 3 IgE-binding bands found in the hair, urine and salivary glands of SH showed a new allergenic protein lacking cross-reactivity with allergens from other rodents. The 3 bands likely correspond to isoforms of a single allergen.

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Animal Lipocalin Allergens

Hilger

Kuehn

Hentges

2012

Curr Allergy Asthma Rep

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Lipocalins represent the most important group of inhalant animal allergens. For some of them, three-dimensional protein structures have been resolved, but their functions are still elusive. Lipocalins generally display a low sequence identity between family members. The characterization of new lipocalin allergens has revealed however that some of them display a high sequence identity to lipocalins from another species. They constitute a new group of potentially cross-reactive molecules which, in addition to serum albumins, may contribute to allergic cross-reactions between animal dander of different species. However, the clinical relevance of cross-reactivity needs to be assessed. Further studies are needed to understand which of these animal lipocalins are the primary allergens and which are cross-reacting molecules. The use of single, well characterized allergens for diagnosis will allow the identification of the sensitizing animal, which is a prerequisite for specific immunotherapy.

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Anaphylaxis after hamster bites – identification of a novel allergen

Abstract: The putative allergen from the hamster saliva causing dwarf hamster-induced anaphylaxis was identified. Possible cross-reactivity with Der p was demonstrated. Further studies will be needed to identify the exact nature and function of this allergen.

Cited by 24 publications

References 3 publications

Male-Specific Submaxillary Gland Protein, a Lipocalin Allergen of the Golden Hamster, Differs from the Lipocalin Allergens of Siberian and Roborovski Dwarf Hamsters

Male-Specific Submaxillary Gland Protein, a Lipocalin Allergen of the Golden Hamster, Differs from the Lipocalin Allergens of Siberian and Roborovski Dwarf Hamsters

An Odorant-Binding Protein as a New Allergen from Siberian Hamster <i>(Phodopus sungorus)</i>

Animal Lipocalin Allergens

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