Anammox Bacterial Abundance, Activity, and Contribution in Riparian Sediments of the Pearl River Estuary

Wang, Shanyun; Zhu, Guibing; Peng, Yongzhen; Jetten, Mike S. M.; Yin, Chengqing

doi:10.1021/es3017446

Cited by 174 publications

(109 citation statements)

References 60 publications

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“…Despite these findings suggesting the functional importance of anammox, the contributions to N 2 production from denitrification were approximately 10e150 times of that from anammox in the BH and LZ columns, and denitrification was responsible for all N 2 emission in the TY column and field-fresh soil. Similar trends are also observed in other paddy soils (Zhu et al, 2011b;Yang et al, 2015), freshwater sediments (Zhu et al, 2011a(Zhu et al, , 2013Wang et al, 2012a) and mangrove sediments (Fernandes et al, 2012), suggesting a more dominant contribution of denitrification than anammox to N 2 production in terrestrial ecosystems. In contrast, the contribution of anammox to N 2 production was reported to exceed denitrification in marine ecosystems (Thamdrup and Dalsgaard, 2002;Engstr€ om et al, 2005).…”

Section: Anammox and Denitrification Activity In The Three Paddy Soilssupporting

confidence: 77%

Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils

Bai

et al. 2015

Soil Biology and Biochemistry

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a b s t r a c tAnaerobic ammonium oxidation (anammox) is a globally important nitrogen-cycling process mediated by specialized microbes, and has been demonstrated to be ubiquitous in anoxic natural settings and bioreactors. However, our knowledge of its prevalence in different paddy soil types and along the depth profiles remains largely undocumented. Here, mesocosm incubations were constructed to investigate the activity and community compositions of anammox bacteria across different depth layers of three different paddy soils. 15N tracer experiments showed that anammox rates were prevalent in almost all depth layers of an inceptisol (Binhai, BH) and an oxisol (Leizhou, LZ), but was absent in an ultisol (Taoyuan, TY). The functional gene hzsB of anammox bacteria was only detected in BH and LZ soil profiles with detected anammox activity. Anammox accounted for 0.4%e12.2% of the total N 2 production, and anammox rates were 0.02e0.77 nmol N g À1 dry soil h À1 and significantly correlated with soil pH and electric conductivity. Anammox activity and abundance of hzsB genes were significantly lower in the surface soil layers (0e5 cm) than in the sub-surface soil layers (20e60 cm). Candidatus Brocadia, Candidatus Kuenenia and Candidatus Jettenia were detected by cloning and sequencing, with Candidatus Brocadia being dominant in all the three soils and Candidatus Jettenia being more frequently detected in the LZ soil. The operational taxonomic unit number and diversity indices of anammox bacterial 16S rRNA gene increased with soil depth. Our results demonstrate that anammox is more common and active in alkaline soils than in acidic soils, in deep paddy soil layers than in surface soil layers, and that anammox activities are likely to be regulated by soil chemical properties such as pH, salinity and redox potential.

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Section: Anammox and Denitrification Activity In The Three Paddy Soilssupporting

confidence: 77%

Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils

Bai

et al. 2015

Soil Biology and Biochemistry

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“…Anammox has been reported to account for Ͼ80% of total N 2 production in the eastern tropical South Pacific oxygen minimum zone (OMZ) off northern Chile (5). Anammox bacteria were detected with broad biogeographic distribution in many naturally occurring anoxic environments, including aquatic ecosystems (6)(7)(8)(9) and terrestrial ecosystems (10)(11)(12)(13)(14)(15). However, their role related to activity in agricultural ecosystems remains poorly understood.…”

mentioning

confidence: 99%

“…The anammox genotype is associated with hydrazine synthase, which consists of three subunits encoded by the genes hzsA, hzsB, and hzsC (21-23), re-sponsible for the synthesis of hydrazine from nitric oxide and ammonium (22). The hzsB gene is a newly developed anammox phylomarker which has been successfully applied in the investigation of the biodiversity and quantitative analyses of anammox bacteria in various environments (9,24,25); hence, hzsB was used in this study.…”

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confidence: 99%

Potential Contribution of Anammox to Nitrogen Loss from Paddy Soils in Southern China

Yang

Nie

et al. 2015

Appl Environ Microbiol

126

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eThe anaerobic oxidation of ammonium (anammox) process has been observed in diverse terrestrial ecosystems, while the contribution of anammox to N 2 production in paddy soils is not well documented. In this study, the anammox activity and the abundance and diversity of anammox bacteria were investigated to assess the anammox potential of 12 typical paddy soils collected in southern China. Anammox bacteria related to "Candidatus Brocadia" and "Candidatus Kuenenia" and two novel unidentified clusters were detected, with "Candidatus Brocadia" comprising 50% of the anammox population. The prevalence of the anammox was confirmed by the quantitative PCR results based on hydrazine synthase (hzsB) genes, which showed that the abundance ranged from 1.16 ؋ 10 4 to 9.65 ؋ 10 4 copies per gram of dry weight. The anammox rates measured by the isotope-pairing technique ranged from 0.27 to 5.25 nmol N per gram of soil per hour in these paddy soils, which contributed 0.6 to 15% to soil N 2 production. It is estimated that a total loss of 2.50 ؋ 10 6 Mg N per year is linked to anammox in the paddy fields in southern China, which implied that ca. 10% of the applied ammonia fertilizers is lost via the anammox process. Anammox activity was significantly correlated with the abundance of hzsB genes, soil nitrate concentration, and C/N ratio. Additionally, ammonia concentration and pH were found to be significantly correlated with the anammox bacterial structure.

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“…As the rice rhizosphere is highly oxygenated compared to bulk soil, nitrite, which is a substrate for anammox, may be produced through both denitrification and nitrification (Lam et al, 2007;Zhu et al, 2011a;Wang et al, 2012a). In oxygenlimited bulk soil with low C/N ratio, where nitrification is inhibited, denitrifiers may reduce limited nitrate diffused from rhizosphere, to nitrite (Trimmer and Nicholls, 2009), then provide anammox with nitrite.…”

Section: Discussionmentioning

confidence: 99%

“…About~3.5 g soil was transferred into a 12 ml gastight vial (Exetainer, Labco, High Wycombe, Buckinghamshire, UK), which were then filled totally with N 2 -purged overlaying water (Wang et al, 2012a) and sealed with a gastight lid. The resulting slurries were pre-incubated for 1 day to remove residual NO x À and oxygen according to our preliminary experiment when both NO 2 À and NO 3 À were under the detection limit of ion chromatograph (0.05e0.1 ppm and 0.075e0.1 ppm for NO 2 À and NO 3 À , respectively).…”

Section: Anammox and Denitrification Rate Measuringmentioning

confidence: 99%

The phenological stage of rice growth determines anaerobic ammonium oxidation activity in rhizosphere soil

Yang

Weng

et al. 2016

Soil Biology and Biochemistry

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a b s t r a c tAnaerobic oxidation of ammonium (anammox) plays an important role in nitrogen (N) loss from agricultural systems. Recently, the rice rhizosphere was demonstrated to be a hotspot for anammox, yet the dynamics of anammox activity and the distribution of anammox bacteria in rhizosphere soil at different phenological stages of rice growth are still unknown. In this study, the activity, diversity and abundance of anammox bacteria in both rhizosphere and bulk soils were investigated over the entire rice growth season. From tillering to ripening stage, significantly higher anammox bacterial abundance was detected in rhizosphere soils compared to bulk soils. The rhizosphere soils also had significantly higher anammox rates at tillering and booting stages (0.71 and 0.32 nmol N g À1 dry soil h À1 , respectively) compared to bulk soils. The anammox rate in rhizosphere soil was positively correlated to the concentrations of NO x À (total of nitrate and nitrite) and acetate. The abundance of anammox bacteria was significantly correlated with the concentration of succinate in rhizosphere soils. A total of five anammox genera of Brocadia, Kuenenia, Anammoxoglobus, Jettenia and Scalindua were detected, with Brocadia predominating in all examined samples. The distribution of anammox bacteria in rhizosphere and bulk soils varied with phenological stages. Statistical analysis indicated that C/N ratio, formate, citrate and ammonium were key factors influencing the composition of anammox bacteria. Variations in activity, abundance and distribution of anammox bacteria in rhizosphere were observed over the phenological progression, demonstrating that the root exudates might be influential for the anammox process. This study implies that future efforts in estimating the rate of anammox should consider the temporal variation during plant life cycles.

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Anammox Bacterial Abundance, Activity, and Contribution in Riparian Sediments of the Pearl River Estuary

Cited by 174 publications

References 60 publications

Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils

Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils

Potential Contribution of Anammox to Nitrogen Loss from Paddy Soils in Southern China

The phenological stage of rice growth determines anaerobic ammonium oxidation activity in rhizosphere soil

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