Anammox bacteria enrichment and denitrification in moving bed biofilm reactors packed with different buoyant carriers: Performances and mechanisms

Gu, Wancong; Wang, Lisheng; Liu, Yanchen; Liang, Peng; Zhang, Xiaoyuan; Li, Yu-You; Huang, Xia

doi:10.1016/j.scitotenv.2020.137277

Cited by 64 publications

(6 citation statements)

References 42 publications

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“…The non-woven material's specific surface and porosity were large, and the membrane module was designed to enhance microorganism retention and treatment efficiency. Non-woven material has been shown to be suitable for Anammox bacteria, which grow slowly, and is effective in maintaining high biomass retention with high effluent quality in Anammox treatment systems (Ren et al 2018;Gu et al 2020). During operation, the maximum TN removal rate achieved was 84.5%, almost the same level as in reports by others (Li et al 2021).…”

Section: Enrichment Of Anammox Sludgesupporting

confidence: 53%

Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor

Lyu

Yan

Gong

et al. 2022

Water Practice and Technology

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An upflow column reactor packed with nonwoven fabric carrier was used to enrich anaerobic ammonium oxidation (Anammox) sludge. After 101 days, the reactor Anammox sludge concentration increased from 470 to 3,118 mg·L−1. In the stable operating stage, the average total nitrogen (TN) volume loading rate was 818.3 mg·L−1, and the maximum removal efficiencies of NH4+-N, NO2−-N and TN were 65.9, 81.2 and 63.8%, respectively. Scanning electron microscopy (SEM) showed that the cultivated sludge was dominated by a mix of short rod-shaped and spherical bacteria, which accumulated to the typical cauliflower-like aggregates assumed to be the Anammox culture. Fluorescence in situ hybridization (FISH) analysis using 16S rRNA showed that the dominant population developed in the reactor when hybridized with both PLA46 and Amx820 gene probes. This indicates that the cultivated biomass may comprise Planctomycetes bacteria. The results of real-time quantitative PCR (qPCR) showed that these bacteria formed 45 to 60% of the total bacteria in the Anammox sludge. The study demonstrated successful detection and enrichment of Anammox bacteria in wastewater.

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Section: Enrichment Of Anammox Sludgesupporting

confidence: 53%

Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor

Lyu

Yan

Gong

et al. 2022

Water Practice and Technology

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“…In mainstream shortcut nitrogen removal systems, a recent study by Van Winckel et al (2019) emphasized the importance of anammox retention for success. One‐stage PdNA applications have been tested so far in MBBRs (Campolong et al, 2019; Gu et al, 2020; Ma et al, 2017) and downflow filters (Fofana, Chen, et al, 2021; Klaus, 2020; Ma et al, 2020; Young et al, 2021), but studies with an IFAS application under real wastewater conditions are limited or missing to the best of our knowledge. One of the benefits offered by an IFAS configuration is the ease and flexibility of implementation into new and existing systems.…”

Section: Introductionmentioning

confidence: 99%

Partial denitrification–anammox (PdNA) application in mainstream IFAS configuration using raw fermentate as carbon source

Ladipo‐Obasa

Forney

Riffat

et al. 2022

Water Environment Research

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This research examined the feasibility of raw fermentate for mainstream partial denitrification-anammox (PdNA) in a pre-anoxic integrated fixed-film activated sludge (IFAS) process. Fermentate quality sampled from a full-scale facility was highly dynamic, with 360-940 mg VFA-COD/L and VFA/soluble COD ratios ranging from 24% to 48%. This study showed that PdNA selection could be achieved even when using low quality fermentate. Nitrate residual was identified as the main factor driving the PdN efficiency, while management of nitrate conversion rates was required to maximize overall PdNA rates. AnAOB limitation was never observed in the IFAS system. Overall, this study showed PdN efficiencies up to 38% and PdNA rates up to 1.2 ± 0.7 g TIN/m 2 /d with further potential for improvements. As a result of both PdNA and full denitrification, this concept showed the potential to save 48-89% methanol and decrease the carbon footprint of water resource recovery facilities (WRRF) by 9-15%. Practitioner points• Application of PdNA with variable quality fermentate is feasible when the nitrate residual concentration is increased to enhance PdN selection.• To maximize nitrogen removed through PdNA, nitrate conversion rates need enhancement through optimization of upstream aeration and PdN control setpoints.• The IFAS PdNA process was never anammox limited; success depended on the degree of PdN achieved to make nitrite available.• Application of PdNA with fermentate can yield 48-89% savings in methanol or other carbon compared with conventional nitrification and denitrification.• Integrating PdNA upstream from polishing aeration and anoxic zones guarantees that stringent limits can be met (<5 mg N/L).

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“…Overall, with an NLR of 0.751 ± 0.022 g-N/(L•d) in stage II, the NRRs of the FTBR were determined to be 0.706 ± 0.021 g-N/(L•d). NRRs of 0.40 g-N/(L•d) were achieved in moving bed biofilm reactors (MBBRs) packed with different buoyant carriers, 29 while 0.23 g-N/(L•d) was also reported in a novel nonwoven fabric membrane bioreactor. 18 During a pilot-scale study of real landfill leachate treatment, a maximum nitrogen removal rate of 0.216 g-N/(L•d) was attained in the anammox process using MBR.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Development of a Flow-through Biofilm Reactor for Anammox Startup and Operation: Nitrogen Removal and Metacommunity

Jin¹,

Xing

Chen³

et al. 2021

ACS EST Water

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A well-designed flow-through biofilm reactor (FTBR) serving as both a biofilter and a biofilm carrier was developed to effectively retain anammox bacteria within threedimensional honeycomb-like textile structures in the anammox process. The bioreactor performance and microbiological properties were investigated under three different fluxes during a period of 160 days. Appreciable biomass aggregates were developed to enhance the nitrogen removal. Accordingly, proper turbidity (≤1 NTU) and nitrogen removal were consistently achieved, in which the FTBR achieved nitrogen removal rates (NRRs) of 0.450 and 0.706 g-N/(L•d) under the nitrogen loading rates (NLRs) of 0.503 and 0.751 g-N/(L•d), respectively. Nitrogen was removed after penetrating the biofilm, and a larger imposed flux on modules resulted in higher nitrogen removal due to the formation of different microenvironments. While the imposed flux increased, the relative abundances of the anammox group increased from 5.23% to 8.12%, whereas the ones of denitrifiers decreased and the ones of ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) did not change significantly. Of the two dominant anammox species, the abundance of Candidatus Kuenenia decreased, whereas the abundance of Candidatus Jettenia increased with increasing flux. Compared to the freely grown biofilm, bacterial communities of flow-through modules showed greater ecological niche differentiation and enhanced mass transport efficiency among species. The developed FTBR system is effective for anammox system startup and optimizing partial nitritation−anammox processes.

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Anammox bacteria enrichment and denitrification in moving bed biofilm reactors packed with different buoyant carriers: Performances and mechanisms

Cited by 64 publications

References 42 publications

Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor

Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor

Partial denitrification–anammox (PdNA) application in mainstream IFAS configuration using raw fermentate as carbon source

Development of a Flow-through Biofilm Reactor for Anammox Startup and Operation: Nitrogen Removal and Metacommunity

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