Analyzing the nitrogen removal performance and cold adaptation mechanism of immobilized cold‐acclimation ANAMMOX granules at low temperatures

Abstract

To improve the treatment performance of anaerobic ammonium oxidation (ANAMMOX) processes at low temperatures, the immobilized cold‐acclimation ANAMMOX granules (R3) were prepared and their low‐temperature nitrogen removal ability as well as the cold adaptation mechanism were analyzed. The results indicated that the total inorganic nitrogen (TIN) removal efficiency of R3 was significantly higher than that of R2 (cold‐acclimation granules without immobilization) and R1 (common granules), especially at 11 ± 2 and 7 ± 2°C (68% and 54%). These were attributed to the remarkable biomass retention capacity of R3, high up to 4.3–4.9 mg/gVSS even at 5–18°C. Besides, higher protein (PN) content of tightly bound extracellular polymeric substances (TB‐EPS) also facilitated microbial aggregation in R3. Meanwhile, R3 granules retained higher ANAMMOX activity and heme c content at 5–25°C. The original dominant ANAMMOX genus (Candidatus Kuenenia) in R3 kept higher abundance (49%–57%) at 23 ± 2 and 16 ± 2°C, whereas Candidatus Brocadia became the dominant ANAMMOX genus (25%–32%) in R3 at 11 ± 2 and 7 ± 2°C. Notably, different ANAMMOX genera in R3 may adapt to cold environment by regulating the expression of cold‐stress proteins (CspA, CspB, PpiD, and UspA).Practitioner Points Immobilized cold‐acclimation ANAMMOX granules showed higher nitrogen removal efficiency at 23°C → 5°C. Immobilization method effectively retained biomass (Candidatus Kuenenia and Candidatus Brocadia). Immobilization facilitated TB‐EPS release and biological aggregation in cold‐acclimation granules. Expression of cold‐stress proteins in immobilized cold‐acclimation granules was more active.