Analyzing the adsorption of blood plasma components by means of fullerene-containing silica gels and NMR spectroscopy in solids

Abstract

The results from studying the adsorption of blood plasma components (e.g., protein, triglycerides, cholesterol, and lipoproteins of low and high density) using silica gels modified with fullerene molecules (in the form of C 60 or the hydroxylated form of C 60 (OH) x ) and subjected to hydration (or, alternatively, dehy dration) are presented. The conditions for preparing adsorbents that allow us to control the adsorption capac ity of silica gel and the selectivity of adsorption toward the components of blood plasma, are revealed. The nature and strength of the interactions of the introduced components (fullerene molecules and water) with functional groups on the silica surface are studied by means of solid state NMR spectroscopy (NMR SS). Conclusions regarding the nature of the centers that control adsorption are drawn on the basis of NMR SS spectra in combination with direct measurements of adsorption. The interaction of the oxygen of the hydroxyl group of silica gel with fullerene, leading to the formation of electron donor complexes of C 60 -H, C 60 -OH, or C 60 -OSi type, is demonstrated by the observed changes in the NMR SS spectra of silica gels in the presence of fullerene.