Analyzing BMP2, FGFR, and TGF Beta Expressions in High-Grade Osteosarcoma Untreated and Treated Autografts Using Proteomic Analysis

Abstract

In the last few decades, biological reconstruction techniques have improved greatly for treating high-grade osteosarcoma patients. To conserve the limb, and its function the affected tumor-bearing bones have been treated using liquid nitrogen and irradiation processes that enable the removal of entire tumors from the bone, and these treated autografts can be reconstructed for the patients. Here, we focus on the expressions of the growth factor family proteins from the untreated and treated autografts that play a crucial role in bone union, remodeling, and regeneration. In this proteomic study, we identify several important cytoskeletal, transcriptional, and growth factor family proteins that showed substantially low levels in untreated autografts. Interestingly, these protein expressions were elevated after treating the tumor-bearing bones using liquid nitrogen and irradiation. Therefore, from our preliminary findings, we chose to determine the expressions of BMP2, TGF-Beta, and FGFR proteins by the target proteomics approach. Using a newly recruited validation set, we successfully validate the expressions of the selected proteins. Furthermore, the increased growth factor protein expression after treatment with liquid nitrogen may contribute to bone regeneration healing, assist in faster recovery, and reduce local recurrence and metastatic spread in high-grade sarcoma patients.

Heterogeneous Analysis of Extracellular Vesicles for Osteosarcoma Diagnosis

“…We applied SPASEC to EV analysis in clinical OS plasma samples from patients with nonmetastatic OS and with metastatic OS. Using the OS EV-related markers (CD63, BMP2, − GD2, N-cadherin), we measured the heterogeneous expression of membrane proteins across distinct subsets of EVs and applied the collected size-related membrane protein expression levels to OS screening by a machine learning algorithm.…”

Molecular and Translational Research on Bone Tumors

“…New technologies, such as proteomic, transcriptomic, and metabolomic analyses, are powerful tools to investigate the molecular mechanisms underlying the development and growth of osteosarcoma. In the research study by Madda et al, a targeted proteomic technology was used to investigate the protein expression of components of the growth factor families (BMP2, bone morphogenetic protein 2; FGFR, fibroblast growth factor receptor; and TGF-beta, transforming growth factor beta) with a fundamental role in bone remodeling and regeneration from treated and untreated autografts [17].…”