Analytik von Gangliosiden unter besonderer Ber�cksichtigung der Milch

Abstract

Gangliosides are sialic acid containing lipid compounds of a very complex nature, which are involved in many physiological processes. Gangliosides are very important constituents of membrane material in animal tissue, where they occur at very low concentrations. Improved analytical procedures are therefore required to study their behaviour, their role in living systems and their isolation for further investigations. In this paper, all analytical methods which may be important in the analysis of gangliosides are summarized and discussed. These include extraction, purification, enrichment and chromatographic procedures. In bovine milk, three gangliosides of known structure are present. Their isolation, structure and analytical importance (buttermilk) are also reported.

Variation in Ganglioside Content of Bovine Dairy Products

“…In addition, the mean value for protocol 4 for sweet buttermilk (356 nmol/g dry wt.) (Table 1) was close to a previously published yield from sweet buttermilk powder (480 nmol/g powder) (Hauttecoeur et al, 1985;Dirks and Reimerdes, 1988). The lower yield of crude ganglioside from cultured buttermilk extracted by protocol 4 (165 nmol/g dry wt.)…”

“…Hauttecoeur et al (1985) found the concentration to be 480$10 nmol of sialic acid/g of lyophilized buttermilk; Takamizawa et al (1986b) reported buttermilk content to be 920 nmol/g dry wt. Dirks and Reimerdes (1988) analyzing gangliosides, found whole milk to have 5.64$1.16 nmol/mL and skim milk to contain 7.5$1.54% of that of whole milk. In this study, using various procedures to extract the gangliosides from buttermilk, a range of 250}719 nmol/g dry wt.…”

“…The lower yield of crude ganglioside from cultured buttermilk extracted by protocol 4 (165 nmol/g dry wt.) was reasonable, since skim milk (the starting material for cultured buttermilk) contains less than 8% of the normal milk gangliosides (Dirks and Reimerdes, 1988). From comparison with our GD standard, the primary component of crude ganglioside mixes from both sweet and cultured buttermilk was GD .…”