Analytical Techniques for the Characterization and Sequencing of Glycosaminoglycans

Sasisekharan, Ram; Shriver, Zachary; Sundaram, Mallik; Venkataraman, Ganesh

doi:10.1002/3527602437.ch20

Cited by 4 publications

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“…Other reviews and review-type articles directly concerned with, or including MALDI analysis of glycoconjugates to have been published during the review period include general reviews on miniaturized separation techniques including LC/MALDI-TOF/TOF , solid-phase tools such as microarrays (Larsen et al, 2006), capillary electrophoresis-MS (Campa et al, 2006;Huck et al, 2006), atmospheric pressure MALDI (Creaser & Ratcliffe, 2006). More specific reviews include those on the analysis of polysaccharides (Cui, 2005), glycoproteins and attached glycans (Aitken, 2005;Morelle & Michalski, 2005;Budnik, Lee, & Steen, 2006;Geiser, Silvescu, & Reinhold, 2006;Geyer & Geyer, 2006;Haslam, Khoo, & Dell, 2006a;Haslam, North, & Dell, 2006b;Kondo et al, 2006;Morelle et al, 2006a), N- (Harcum, 2005;Harvey, 2005d,e;Medzihradszky, 2005;Jang-Lee et al, 2006) and O-linked (Peter-Katalinic, 2005) glycosylation, bacterial glycoproteomics , protein glycation Niwa, 2006;Silván et al, 2006), GPI anchors (Baldwin, 2005), proteoglycans , glycosylaminoglycans (Gama & Hsieh-Wilson, 2005;Pojasek, Raman, & Sasisekharan, 2005;Sasisekharan et al, 2006), glycosphingolipids (Levery, 2005;Zheng, Wu, & Hancock, 2006b), and flavonoids (de Rijke et al, 2006). The book on mass spectrometry in biophysics by Kaltashov and Eyles (2005) also contains information.…”

Section: Introductionmentioning

confidence: 97%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for the period 2005–2006

Harvey

2010

Mass Spectrometry Reviews

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This review is the fourth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2006. The review covers fundamental studies, fragmentation of carbohydrate ions, method developments, and applications of the technique to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, glycated proteins, glycolipids from bacteria, glycosides, and various other natural products. There is a short section on the use of MALDI-TOF mass spectrometry for the study of enzymes involved in glycan processing, a section on industrial processes, particularly the development of biopharmaceuticals and a section on the use of MALDI-MS to monitor products of chemical synthesis of carbohydrates. Large carbohydrate-protein complexes and glycodendrimers are highlighted in this final section.

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Section: Introductionmentioning

confidence: 97%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for the period 2005–2006

Harvey

2010

Mass Spectrometry Reviews

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Sulfatasen: Struktur, Mechanismus, biologische Aktivität, Inhibition, Anwendung in Synthesen

2004

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Sulfatasen – d. h. Sulfatester spaltende Enzyme – spielen eine entscheidende Rolle bei der Regulierung von Sulfatierungszuständen, die über die Funktion vieler physiologischer Moleküle entscheiden. Die Substrate der Sulfatasen reichen von kleinen cytosolischen Steroiden wie dem Östrogensulfat bis hin zu komplexen Kohlenhydraten auf Zelloberflächen, etwa den Glycosaminoglycanen. Die Umwandlung dieser Moleküle wurde mit wichtigen zellulären Funktionen in Verbindung gebracht, z. B. mit der Hormonregulation, dem zellulären Abbau und der Modulation von Signalisierungsprozessen. Sulfatasen hängen mit dem Ausbruch etlicher pathophysiologischer Erscheinungen zusammen, etwa mit hormonabhängigen Krebsformen. Bis heute sind zwar zahlreiche Sulfatasen identifiziert worden, die Aufklärung des weitreichenden Spektrums ihrer biologischen Aktivitäten befindet sich aber erst am Anfang. Dieser Aufsatz gibt einen Überblick über den derzeitigen Wissensstand zu den Strukturen, Mechanismen und der Inhibition von Sulfatasen.

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Sulfotransferases: Structure, Mechanism, Biological Activity, Inhibition, and Synthetic Utility

et al. 2004

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The sulfonation (also known as sulfurylation) of biomolecules has long been known to take place in a variety of organisms, from prokaryotes to multicellular species, and new biological functions continue to be uncovered in connection with this important transformation. Early studies of sulfotransferases (STs), the enzymes that catalyze sulfonation, focused primarily on the cytosolic STs, which are involved in detoxification, hormone regulation, and drug metabolism. Although known to exist, the membrane-associated STs were not studied as extensively until more recently. Involved in the sulfonation of complex carbohydrates and proteins, they have emerged as central players in a number of molecular-recognition events and biochemical signaling pathways. STs have also been implicated in many pathophysiological processes. As a result, much interest in the complex roles of STs and in their targeting for therapeutic intervention has been generated. Progress in the elucidation of the structures and mechanisms of sulfotransferases, as well as their biological activity, inhibition, and synthetic utility, are discussed in this Review.

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Analytical Techniques for the Characterization and Sequencing of Glycosaminoglycans

Cited by 4 publications

References 0 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for the period 2005–2006

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for the period 2005–2006

Sulfatasen: Struktur, Mechanismus, biologische Aktivität, Inhibition, Anwendung in Synthesen

Sulfotransferases: Structure, Mechanism, Biological Activity, Inhibition, and Synthetic Utility

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