Analytical techniques for determining biotin

Simultaneous determination of water‐soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry

“…The isotopic distributions of the doubly and singly charged ions are depicted in (A) and (B), respectively; the reciprocal of the distance between any two isotope peaks allows the easy determination of the charge state of each adduct. the number of 13 C peaks or by evaluating the reciprocal of the mass difference between any two adjacent 13 C peaks in the isotope envelope: in this way, in the TOF mass spectra (Figs. 3(A) and 3(B)) the doubly and singly charged ions of vitamin B12, respectively, can be easily recognized.…”

“…10,11 The naturally active form of vitamin B8 is D-(þ)-biotin, which occurs in foodstuffs both free and covalently bound to proteins. Acid hydrolysis (1-3 M H 2 SO 4 by autoclaving at 1218C) is the treatment most used to break these bonds, 12,13 while enzymatic hydrolysis with papain is considered less effective. 12 In food (especially in vegetables) folic acid is mainly bound to oligo-g-L-glutamates made up of 2-6 glutamic acid residues.…”

Development of an Egg-white Bioassay for Monitoring Biotin Levels in Urine and Serum

“…The novelty of our method is that avidin was allowed to react without isolating it from egg white where it is normally found. Although the sensitivity of our method is lower than the sensitivity reported for sophisticated microbiological or high performance liquid chromatography (HPLC) methods (10 -9 to 10 -8 M), 5 our method is a simple, cheap and easy to use technique when compared with other bioassays, which require animal feeding and the determination of the concentration of biotin is based on the animal development curve.…”

“…Despite the vast number of techniques that are available at the moment, none is used routinely due to their sophistication, cost, and the presence of interferents, the most common of which are biotin metabolites. 5 The aim of this study was the development of a simple, easy to apply and cost effective bioassay for the possible detection of biotin in biological samples like urine and serum. The method was based on the very selective binding of avidin and biotin.…”

Determination of biotin in Antarctic krill (Euphausia superba) by high‐performance TLC with different post‐chromatographic derivatizations

“…Microbiological assays are sensitive, but the sample's nutrients may promote certain micro-organisms' growth and interfere with the assay results [40]. In addition, it is also rather tedious, time consuming, and expensive [41].…”