Analytical Techniques for Determination of Hydrochlorothiazide and its Combinations: A Review

Savaj, Bhadresh V.; Patidar, Ashutoshkumar; Raj, Hasumati A.

doi:10.7439/ijasr.v1i3.1786

Cited by 5 publications

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References 50 publications

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“…An extensive literature survey was carried out and found that there are few analytical methods for the determination of hydrochlorothiazide alone or in its combination with other drugs in pharmaceutical preparations including spectrophotometry [3,4], flow injection [5], TLC [6], and HPLC [7][8][9]. A recent study involved a review of developing methods using these analytical techniques for estimation of hydrochlorothiazide in various biological samples with focus on sample pretreatment methods and separation methods [10].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

Mohammed

2016

Chromatography Research International

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An HPLC-PDA method was developed and validated for the determination of hydrochlorothiazide in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 50 : 50 acetonitrile : water, Inertsil5 column (ODS-3 250 mm × 4.6 mm 5 m), 20 L injection volume, flow rate of 1 mL/min at ambient temperature (30 ∘ C), and 272 nm. Another column of C 18 Zorbax5 (Eclipse Plus, 4.6 × 250 mm, 5 m) was tested showing no big difference in the method results. The method was validated giving good precision (RSD% < 1), acceptable linearity ( 2 ≥ 0.9978), and low LOD and LOQ (0.5 and 1.7 g/mL, resp.) on both columns. Successful application on pharmaceutical dosage tablet form gave high recovery of 99.93%. The method was compared with official BP and other reported methods. The proposed method is economic, simple, and rapid and hence can be employed for routine analysis in quality control laboratories. Materials and Methods2.1. HPLC System. Analytical HPLC system comprised a Flexar autosampler injector-no Peltier, Flexar Binary pump,

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Section: Introductionmentioning

confidence: 99%

Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

Mohammed

2016

Chromatography Research International

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Advancing USP compendial methods for fixed dose combinations: A case study of metoprolol tartrate and hydrochlorothiazide tablets

2019

Journal of Pharmaceutical Analysis

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The current United States Pharmacopeia–National Formulary (USP–NF) includes more than 250 monographs of fixed dose combinations (FDCs), and some of them need to be updated due to incompleteness of impurity profiles and obsolescence of analytical methodologies. A case study of metoprolol tartrate and hydrochlorothiazide tablets is presented to summarize challenges encountered during the USP monograph modernization initiative of FDCs and to highlight an “adoption and adaptation” approach employed for method development. To this end, a single stability-indicating HPLC method was developed to separate the two drug substances and eight related compounds with resolution 2.0 or higher between all critical pairs. Chromatographic separations were achieved on a Symmetry column (C 18 , 100 mm × 4.6 mm, 3.5 µm) using sodium phosphate buffer (pH 3.0; 34 mM) and acetonitrile as mobile phase in a gradient elution mode. The stability-indicating capability of this method has been demonstrated by analyzing stressed samples of the two drug substances. The developed HPLC method was validated for simultaneous determination of metoprolol tartrate and hydrochlorothiazide and relevant impurities in the tablets. Moreover, the developed method was successfully applied to the analysis of commercial tablet dosage forms and proved to be suitable for routine quality control use. The case study could be used to streamline USP's monograph modernization process of FDCs and strengthen compendial procedures.

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Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride

2020

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Green and sensitive spectrofluorometric methods have been developed and validated for the determination of timolol maleate (TML)/hydrochlorothiazide (HCT) and amiloride hydrochloride (AMH)/hydrochlorothiazide in tablets. The proposed spectrofluorometric procedures were found to be linear in the range of 4–12, 5–35 and 0.025–0.2 mg L−1 for HCT, TML and AMH, resp. The excitation and emission wavelengths for HCT, TML and AMH at room temperature were 270 and 375, 295 and 435, 330 and 415 nm, resp. The methods were validated with respect to ICH guidelines. The AMH showed higher sensitivity with lower values of LOD and LOQ values compared to HCT and TML. The proposed methods were applied to two pharmaceutical formulations; the method for HCT and AMH has proven as reliable assaying method, whereas the method for TML, when combined with HCT, is applicable to screening semi-quantitative analyses.

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Analytical Techniques for Determination of Hydrochlorothiazide and its Combinations: A Review

Cited by 5 publications

References 50 publications

Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

Advancing USP compendial methods for fixed dose combinations: A case study of metoprolol tartrate and hydrochlorothiazide tablets

Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride

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