Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography

Siakotos, A. N.; Rouser, George

doi:10.1007/bf02632444

Cited by 428 publications

(100 citation statements)

References 16 publications

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“…The glycolipid fraction was freed of trace impurities through TLC on SI-gel G plates employing the solvent system CHCl 3 -acetone-MeOH-AcOH-H 2 0 5:2:1:1:0.5 (v/v). Identification of individual glycolipids was carried out by running authentic reference standards simultaneously and also by staining with á-naphthol reagent (Siakotes LIPID PROFILE DURING GROWTH AND SENESCENCE OF Catharanthus roseus and Rouser, 1965) and water spray (used to facilitate observations of translucent areas on the TLC plates) (Gardner, 1968) was employed for identification and analytical purposes. The separated glycolipids were estimated on the basis of sugar released after hydrolysis (Roughan and Batt, 1968).…”

Section: Methodsmentioning

confidence: 99%

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Mishra¹,

Tyagi²,

Singh³

et al. 2006

Braz. J. Plant Physiol.

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Various lipid classes and compounds were monitored during the period of leaf emergence to leaf drop of Catharanthus roseus. The expansion to early maturation phase was accompanied by cellular build-up of all major lipid classes, whilst aging and senescence were characterized by their substantial decline, except for the neutral lipids; the leaf monogalactosyl diglyceride/digalactosyl diglyceride ratio decreased from 4.3 (complete maturity) to 2.1 (abscised stage). The early maturation stage was the earliest stage when appreciable amounts of free sterols and fatty acids could be observed. Sterol/phospholipids ratios increased by 68-fold in the abscised leaf as compared to that at full maturity. The unsaturated/saturated fatty acid ratio was far lower in the senescent leaf as compared to that of the fully expanded leaf. The spatial alterations in lipid profiles may be suggestive of concomitant changes in membrane ultrastructure and functions, putatively leading to perturbation of indole alkaloid sequestration capability of the tissues of a species of pharmaceutical significance. Key words: Catharanthus roseus, glycolipids, leaf development, phospholipids, senescence, sterol Alterações no perfil de lipídeos durante o crescimento e a senescência de folhas de Catharanthus roseus: Rastrearamse alterações na composição de vários compostos e classes de lipídeos em folhas de Catharanthus roseus, desde a emergência até a abscisão. Da expansão ao início da maturação da folha, houve incrementos nas concentrações de todas as principais classes de lipídeos, observando-se tendência oposta na senescência, à exceção de lipídeos neutros; a razão monogalactosil diglicerídeo/digalactosil diglicerídeo decresceu de 4.3 (maturidade completa) para 2.1 (abscisão). Quantidades apreciáveis de esteróis e ácidos graxos começaram a ser observadas apenas no início maturação foliar. A razão esteróis/fosofolipídeos aumentou 68 vezes na fase de abscisão em relação à folha em sua completa maturidade. A razão ácidos graxos insaturados/ácidos graxos saturados foi muito menor na folha senescente que na folha jovem completamente expandida. As alterações espaciais no perfil de lipídeos podem ser uma sugestão de alterações concomitantes na função e ultra-estrutura da membrana, acarretando, putativamente, perturbações na capacidade de seqüestro de alcalóides dos tecidos de uma espécie importante, de um ponto de vista farmacêutico.

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Section: Methodsmentioning

confidence: 99%

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Mishra¹,

Tyagi²,

Singh³

et al. 2006

Braz. J. Plant Physiol.

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“…for the second dimension. Three spray reagents were applied to detect polar lipids (Tindall, 1990): 10 % ethanolic molybdophospholic acid reagent for total lipids, ninhydrin reagent for aminogroup-containing lipids, Zinzadze reagent for phospholipids and a-naphthol reagents for glycolipids (Siakotos & Rouser, 1965). The phospholipids were identified by one-dimensional TLC with chloroform/methanol/acetic acid/water (50 : 6 : 6 : 1, by vol.)…”

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confidence: 99%

Arthrobacter echini sp. nov., isolated from the gut of a purple sea urchin, Heliocidaris crassispina

Lee

Hyun

Kim

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel strain, designated AM23 T , was isolated from the gut of a purple sea urchin Heliocidaris crassispina collected from the coastal waters of the Korean island Dokdo. 16S rRNA gene sequence analysis showed that strain AM23 T belonged to the genus Arthrobacter in the family Micrococcaceae and shared highest sequence similarity with Arthrobacter agilis DSM 20550 T (98.77 %). Strain AM23 T was catalase-positive, oxidase-negative and grew optimally at 20 8C, in the presence of 1 % (w/v) NaCl and at pH 7. The isolate was a Gram-stain-positive, non-motile, strictly aerobic and coccus-shaped bacterium. The major cellular fatty acids were anteiso-C 15 : 0 and iso-C 15 : 0 . The polar lipids of strain AM23 T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and four unidentified lipids. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine and the predominant cell-wall sugars were galactose, mannose, rhamnose and ribose. The major respiratory quinone was identified as menaquinone MK-9(H 2 ). The genomic DNA G+C content was 67.3 mol% and the DNA-DNA hybridization values showed the strain shared less than 29 % genomic relatedness with A. agilis DSM 20550 T . The results of the phylogenetic, phenotypic and genotypic analysis indicate that strain AM23 T represents a novel species in the genus Arthrobacter, for which the name Arthrobacter echini sp. nov. is proposed. The type strain is AM23 T (5KACC 18260 T 5DSM 29493 T ).

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“…Samples were analyzed on silica gel 60 aluminum-backed high performance thin layer chromatography plates (Merck). A section of the plate was stained with ␣-naphthol reagent and heated at 110°C for 5 min to detect the product (26).…”

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confidence: 99%

Biosynthesis of the Linkage Region of Glycosaminoglycans

Bai

Zhou

Brown

et al. 2001

Journal of Biological Chemistry

161

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A family of five ␤1,3-galactosyltransferases has been characterized that catalyze the formation of Gal␤1, 3GlcNAc␤ and Gal␤1,3GalNAc␤ linkages present in glycoproteins and glycolipids (␤3GalT1, -2, -3, -4, and -5). We now report a new member of the family (␤3GalT6), involved in glycosaminoglycan biosynthesis. The human and mouse genes were located on chromosomes 1p36.3 and 4E2, respectively, and homologs are found in Drosophila melanogaster and Caenorhabditis elegans. Unlike other members of the family, ␤3GalT6 showed a broad mRNA expression pattern by Northern blot analysis. Although a high degree of homology across several subdomains exists among other members of the ␤3-galactosyltransferase family, recombinant enzyme did not utilize glucosamine-or galactosamine-containing acceptors. Instead, the enzyme transferred galactose from UDP-galactose to acceptors containing a terminal ␤-linked galactose residue. This product, Gal␤1,3Gal␤ is found in the linkage region of heparan sulfate and chondroitin sulfate (GlcA␤1,3Gal␤1,3Gal␤1,4Xyl␤-O-Ser), indicating that ␤3GalT6 is the so-called galactosyltransferase II involved in glycosaminoglycan biosynthesis. Its identity was confirmed in vivo by siRNA-mediated inhibition of glycosaminoglycan synthesis in HeLa S3 cells.

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Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography

Cited by 428 publications

References 16 publications

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Changes in lipid profile during growth and senescence of Catharanthus roseus leaf

Arthrobacter echini sp. nov., isolated from the gut of a purple sea urchin, Heliocidaris crassispina

Biosynthesis of the Linkage Region of Glycosaminoglycans

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