Analytical procedures for determination of phenolics active herbal ingredients in fortified functional foods: an overview

Sik, Beatrix; Székelyhidi, Rita; Lakatos, Erika; Kapcsándi, Viktória; Ajtony, Zsolt

doi:10.1007/s00217-021-03908-6

Cited by 10 publications

(4 citation statements)

References 92 publications

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“…These compounds are present in plants in the free form and as ester and/or glycosidic derivatives. However, the health effects of bioactive polyphenols are determined by their bioavailability which is influenced by many factors, including phenolic structure, chemical interactions, food processing and the food matrix components [ 12 , 13 ]. There is also no information about free and conjugated phenolic compounds in different herbs.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Profile of Phenolic Compounds in Selected Herbal Plants

Foss

Przybyłowicz

Sawicki

2022

Plant Foods Hum Nutr

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Herbs are characterized by a high content of biologically active substances that positively affect human health. Phenolic compounds are one of the main bioactive compounds in these plants with highly beneficial properties (e.g., anti-carcinogenic, cardioprotective, immune system support and antibacterial). Therefore, this study aimed to determine the composition of free and bound phenolic compounds and antioxidant activity in 10 different herbs (bogbean leaves, sage leaves, whole Indian hemp, whole heartsease, whole horsetail, whole blessed thistle, whole thyme, chamomile flower, corn silk and pine buds). Phenolic compounds were analyzed using the HPLC-TOF-MS/MS method, total phenolic (TP) and flavonoid (TF) contents were measured using spectrophotometric methods, while antioxidant activity was determined by ABTS and DPPH assays. The highest content of free TP was found in thyme, while sage was characterized by the highest range of these compounds released from ester and glycosidic derivatives by hydrolysis. In turn, the highest values of TF in non-hydrolyzed extracts were found in Indian hemp. The highest values of TF, including bound phenols were observed in extracts obtained from sage leaves. In the analyzed herbs thirty-three phenolic compounds were found, constituting 16 phenolic acids, 9 flavonoids and eight stilbenes. Moreover, the analyzed compounds appeared in the bound form than in the free form. The antioxidant activity of herb extracts differed significantly across varieties (P < 0.05). The research showed that each tested herb possesses its own fingerprint of phenolic compounds and antioxidant properties.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Profile of Phenolic Compounds in Selected Herbal Plants

Foss

Przybyłowicz

Sawicki

2022

Plant Foods Hum Nutr

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“…In light of these shortcomings, high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) appears to be the most commonly employed technique for the qualitative and quantitative analyses of phenolic compounds [ 1 , 8 , 16 , 58 ]. Although the set up of HPLC–DAD is relatively cheap and the analysis robust, the method nonetheless faces several disadvantages, such as i) compound identification is only based on retention times and associated UV spectra, which might not allow us to sufficiently discriminate between compounds of a very similar chemical nature, and ii) low detection and quantification limits when analysing complex matrices [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

The Development and Application of a HPTLC-Derived Database for the Identification of Phenolics in Honey

et al. 2022

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This study reports on the development and validation of a HPTLC-derived database to identify phenolic compounds in honey. Two database sets are developed to contain the profiles of 107 standard compounds. Rich data in the form of Rf values, colour hues (H°) at 254 nm and 366 nm, at 366 nm after derivatising with natural product PEG reagent, and at 366 nm and white light after derivatising with vanillin–sulfuric acid reagent, λ max and λ min values in their fluorescence and λ max values in their UV-Vis spectra as well as λ max values in their fluorescence and UV-Vis spectra after derivatisation are used as filtering parameters to identify potential matches in a honey sample. A spectral overlay system is also developed to confirm these matches. The adopted filtering approach is used to validate the database application using positive and negative controls and also by comparing matches with those identified via HPLC-DAD. Manuka honey is used as the test honey and leptosperine, mandelic acid, kojic acid, lepteridine, gallic acid, epigallocatechin gallate, 2,3,4-trihydroxybenzoic acid, o-anisic acid and methyl syringate are identified in the honey using the HPTLC-derived database.

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“…The popularity of this assay can mainly be attributed to its simplicity and speed of analysis, but it presents low specificity, as the color reaction can occur with any oxidizable phenolic hydroxy group, especially if they are present in large amounts [ 88 ]. In fact, other substances such as ascorbic acid, aromatic amines, reducing sugars, SO 2 , tyrosine [ 84 , 89 ], vitamins, amino acids, proteins, organic acids [ 90 ], and the presence of Fe(III) [ 86 ] interfere with the results of the Folin–Ciocalteu assay and they are inadvertently quantified as polyphenols, skewing the results. However, this method continues to be widely applied in food and plant science to measure total phenolics due to its ease of use and low cost.…”

Section: Polyphenols and Their Obtentionmentioning

confidence: 99%

A Comprehensive Analytical Review of Polyphenols: Evaluating Neuroprotection in Alzheimer’s Disease

Vicente-Zurdo,

Gómez-Mejía,

Rosales-Conrado

et al. 2024

IJMS

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Alzheimer’s Disease (AD), a prevalent neurodegenerative disorder, is the primary cause of dementia. Despite significant advancements in neuroscience, a definitive cure or treatment for this debilitating disease remains elusive. A notable characteristic of AD is oxidative stress, which has been identified as a potential therapeutic target. Polyphenols, secondary metabolites of plant origin, have attracted attention due to their potent antioxidant properties. Epidemiological studies suggest a correlation between the consumption of polyphenol-rich foods and the prevention of chronic diseases, including neurodegenerative disorders, which underscores the potential of polyphenols as a therapeutic strategy in AD management. Hence, this comprehensive review focuses on the diverse roles of polyphenols in AD, with a particular emphasis on neuroprotective potential. Scopus, ScienceDirect, and Google Scholar were used as leading databases for study selection, from 2018 to late March 2024. Analytical chemistry serves as a crucial tool for characterizing polyphenols, with a nuanced exploration of their extraction methods from various sources, often employing chemometric techniques for a holistic interpretation of the advances in this field. Moreover, this review examines current in vitro and in vivo research, aiming to enhance the understanding of polyphenols’ role in AD, and providing valuable insights for forthcoming approaches in this context.

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Analytical procedures for determination of phenolics active herbal ingredients in fortified functional foods: an overview

Cited by 10 publications

References 92 publications

Antioxidant Activity and Profile of Phenolic Compounds in Selected Herbal Plants

Antioxidant Activity and Profile of Phenolic Compounds in Selected Herbal Plants

The Development and Application of a HPTLC-Derived Database for the Identification of Phenolics in Honey

A Comprehensive Analytical Review of Polyphenols: Evaluating Neuroprotection in Alzheimer’s Disease

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