Analytical performances of Optilite® turbidimeter (The Binding Site): a new dedicated analyser for specific proteins determination

Antigen excess detection by automated assays for free light chains

“…samples with previously demonstrated antigen excess when assayed by Freelite on BNII [6][7][8][9]. In studies performed at UZ Brussel and Pitié-Salpêtrière, Optilite failed to detect antigen excess in 1/8 and 0/3 [7] κ and 1/1 and 1/3 [7] λ cases of antigen excess with Freelite on BNII, respectively. In previous studies, N Latex Siemens antigen detection systems did not fail to detect antigen excess in 19 κ (13 [6], 4 [8], 2 [9]) and 16 λ [6] selected samples with antigen excess when assayed by Freelite on a BN system [6,8,9].…”

“…Most previous studies investigated antigen excess on Siemens or Beckman analyzers without antigen excess detection for Freelite reagents [3][4][5]. Only few data are available on the performance of antigen excess detection using Freelite on SPA Plus [6] or Optilite [7] [Vercammen, Optilite Seed Site Meeting, Birmingham March 2016] and on BN systems using N Latex [6,8,9]. In the current study, we evaluated whether and to what extent the Freelite FLC assay applied on automated Optilite instrument and the N Latex assay applied on a BN system suffer from antigen excess.…”

“…Previous studies only reported on antigen excess detection by Freelite on Optilite or SPA Plus and by N Latex on a BN system in a limited set of selected samples, i.e. samples with previously demonstrated antigen excess when assayed by Freelite on BNII [6][7][8][9]. In studies performed at UZ Brussel and Pitié-Salpêtrière, Optilite failed to detect antigen excess in 1/8 and 0/3 [7] κ and 1/1 and 1/3 [7] λ cases of antigen excess with Freelite on BNII, respectively.…”

Stability study and external quality assessment scheme implementation for complement components dosage on EDTA plasma

“…Les kits de dosage des fractions C3 et C4 sont quant à eux calibrés par rapport au matériel de référence ERM-DA470k/IFCC, développé pour l'harmonisation du dosage des protéines [13]. Les performances de l'automate Optilite ® pour le dosage des fractions C3 et C4 ont déjà été décrites précédemment [14], et la validation du dosage du C1-inhibiteur a fait l'objet d'une publication récente [15]. Selon notre expérience, les coefficients de variation (CV) de répétabilité et de fidélité intermédiaire sont respectivement inférieurs à 3,0 et 5,0 % pour les 4 paramètres.…”