Analytical Performance of Polymer-Based Microfluidic Devices Fabricated By Computer Numerical Controlled Machining

Mecomber, Justin S.; Stalcup, Apryll M.; Hurd, Doug; Halsall, H. Brian; Heineman, William R.; Seliskar, Carl J.; Wehmeyer, Kenneth R.; Limbach, Patrick A.

doi:10.1021/ac051523y

Cited by 29 publications

(23 citation statements)

References 37 publications

(49 reference statements)

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“…25 The channel cross section was 100 lm (width) by 30 lm (depth), and effective separation length was set at 30 mm. The distances from the intersection of the microchannels to the buffer reservoir, buffer waste reservoir, sample reservoir, and sample waste reservoir were 5.7, 37.5, 5.2, and 5.2 mm, respectively.…”

Section: Microchip Electrophoresismentioning

confidence: 99%

Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips

et al. 2011

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In this paper, we study characteristics of polymers (methylcellulose, hypromellose ((hydroxypropyl)methyl cellulose), poly(vinylpyrrolidone), and poly(vinyl alcohol)) with different chemical structures for microchip electrophoresis of non-denatured protein samples in a plastic microchip made of poly(methyl methacrylate) (PMMA). Coating efficiency of these polymers for controlling protein adsorption onto the channel surface of the plastic microchip, wettability of the PMMA surface, and electroosmotic flow in the PMMA microchannels in the presence of these polymers were compared. Also relative electrophoretic mobility of protein samples in solutions of these polymers was studied. We showed that when using low polymer concentrations (lower than the polymer entanglement point) where the sieving effect is substantially negligible, the interaction of the samples with the polymer affected the electrophoretic mobility of the samples. This effect can be used for achieving better resolution in microchip electrophoresis of protein samples.

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Section: Microchip Electrophoresismentioning

confidence: 99%

Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips

et al. 2011

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“…The conventional computer numerical controlled (CNC) machines like lathes and mills are very common tools in most of the universities and they were used to fabricate polymer microfluidic devices for chemical applications. 7,8 Disadvantages of using CNC machine for polymer microfluidic devices have been reported like less accuracy, higher surface roughness, round corner feature, 9,10 and researchers improved the manufacturing process of conventional CNC machining 8 or used micromachining [11][12][13] to overcome those disadvantages, leading to a costefficient tool in manufacturing disposable microfluidic platforms.…”

Section: Introductionmentioning

confidence: 99%

Parameter optimization of micromilling brass mold inserts for microchannels with Taguchi method

Chen

Pan

et al. 2015

Int. J. Precis. Eng. Manuf.

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Achieving excellent quality of micromilled surfaces usually requires additional efforts and cost, and Taguchi methodology is an efficient tool in the parameter optimization process. The aim of this study is to explore the optimal cutting parameters for minimal surface roughness of a micromilled brass mold inset, and the controlling parameters included coolant, spindle speed, feed rate, depth of cut, and the stepover. An orthogonal array and factor analysis were carried out to identify the optimal cutting combination, and this combination included air coolant, spindle speed of 45,000 rpm, feed rate of 100 mm/min, stepover of 10% of milling bit diameter, and the depth of cut of 5 µm. To further confirm the analysis, multiple confirmation runs were realized and the averagely measured surface roughness was 0.03 µm with a stand deviation of 0.004 µm.

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“…7,8 Low aspect ratio features can be easily produced via soft lithography techniques, [9][10][11][12] however, they require clean room access which can be costly. 13 Micro milling, or precision milling, 14,15 approaches are appealing for the fabrication of biochemical microdevices; micro milling offers a low budget and robust approach for the micromachining of moulds with different feature sizes, and its machinery is typically more accessible.…”

Section: Introductionmentioning

confidence: 99%

condiCharacterisation of an Adhesive-free Packaging System for Polymeric Microfluidic Biochemical Devices and Reactors

Reichen

Super

Davies

et al. 2014

Chem.Biochem.Eng.Q.

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The development of microfluidic devices is an iterative process that involves series of improvements, which can be costly and time consuming. We present a packaging system which makes use of an accessible rapid prototyping method, and facilitates the rapid and reliable implementation of polymeric microfluidic device designs. The packaging system uses a modular design and is based on an adhesive-free connection of a reusable and stiff polymeric interface plate with a disposable, soft microfluidic chip under compression. We characterised the system by numerically and experimentally studying the effect of compression and key dimensions on burst pressure and flow rate. All parts are fabricated with readily available low-cost materials and micro-milling technology. The presented approach is both facilitating and systematising the fabrication of devices with different degrees of complexity; keeping assembly and interconnection simple and straightforward. Furthermore, minimising the time between a design and a finished working prototype yields rapid verification of microfluidic design concepts and testing of assays. Several chip designs were fabricated, then growth of stem cells and hydrodynamic vertical flow focusing in a microfluidic device were realised using our approach. Our approach minimises the need for re-development and re-testing of interface components; reducing cost and time requirements.

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Analytical Performance of Polymer-Based Microfluidic Devices Fabricated By Computer Numerical Controlled Machining

Cited by 29 publications

References 37 publications

Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips

Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips

Parameter optimization of micromilling brass mold inserts for microchannels with Taguchi method

condiCharacterisation of an Adhesive-free Packaging System for Polymeric Microfluidic Biochemical Devices and Reactors

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