Analytical Performance of BÜHLMANN fPELA® turbo, a Fecal Pancreatic Elastase PETIA Assay

Winter, Ian W.; Guschin, D.; Vogt, David; Yang, Jimin; Huk, Andriy; Weber, Jakob; Keller, E.; Schuster, T; Nilsen, Turid; Schneider, M

doi:10.1016/j.pan.2020.07.205

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“…1 A semi-automated, particle-enhanced, open-channel turbidimetric immunoassay has been introduced by Bühlmann (fPELA turbo, Bühlmann Laboratories AG, Schoenenbuch, Switzerland). 2 The Optilite is a benchtop analyser available from the Binding Site (Edgbaston, Birmingham, UK) which is optimized for protein analysis and has greater than 50 serum protein assays available. Furthermore, several third party assays are available for use and is therefore well suited for small to medium throughput laboratories.…”

Section: Introductionmentioning

confidence: 99%

Verification of the Bühlmann fPELA turbo faecal elastase assay on the Binding Site Optilite benchtop analyser

et al. 2022

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Background Pancreatic elastase-1 (PE1) can be measured to assess exocrine activity of the pancreas. A semi-automated particle enhanced, open-channel turbidimetric immunoassay has been introduced by Bühlmann (fPELA turbo, Bühlmann Laboratories AG, Schoenenbuch, Switzerland). Published evaluation data is lacking. We therefore verified performance of the assay on the Binding Site Optilite benchtop analyser and undertook a sample comparison with the DiaSorin PE1 assay on the Liaison. Methods Accuracy, imprecision, lower limit of quantitation (LLoQ), and linearity of the Bühlmann fPELA turbo assay on the Binding Site Optilite analyser was ascertained. Comparison with the DiaSorin Liaison PE1 assay was also undertaken. Difference between assays was evaluated using the Wilcoxon signed-rank test and method comparison was undertaken using Spearman’s rank correlation (rs), Bland-Altman and Passing-Bablok regression analyses. Results The fPELA turbo assay was linear between 5 and 2500 μg/g. The LLoQ was 5 µg/g. Intra-, and inter-assay imprecision was <6%. There was a good agreement (rs = 0.92) and no significant bias (5.8 µg/g, P = 0.29) present between the Bühlmann fPELA turbo and DiaSorin PE1 assays. Conclusion The Bühlmann fPELA turbo assay performs well on the Binding Site Optilite analyser. Faecal elastase results are commutable between with Bühlmann fPELA turbo and DiaSorin Liaison PE1 assays.

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Section: Introductionmentioning

confidence: 99%