Analytical Methods of Isolation and Identification

Feng, Weisheng; Li, Meng; Hao, Zhi‐You; Zhang, Jingke

doi:10.5772/intechopen.88122

Cited by 26 publications

(19 citation statements)

References 28 publications

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“…The AE showed the presence of metabolites belonged to different phytochemical families such as flavonols, methoxyflavonols, flavones, methoxyflavones, and hydroxycinnamic acids. It is known the water is a solvent with the capacity to dissolve compounds with high polarity 39 . In this case, the metabolites found in the AE are phenolic compounds whose chemical structure contains hydroxyl and ketone groups which causes a strong polarity in these molecules that makes them affine to the high polarity of water 38 .…”

Section: Resultsmentioning

confidence: 99%

Phytochemical and Biological Characterization of Aqueous and Ethanolic Extracts of Parthenium hysterophorus

Jiménez¹,

Zugasti–Cruz²,

Belmares³

et al. 2021

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For a long time, plants have been used as traditional treatments for diverse health issues. Their medicinal properties are associated with the presence of phytochemical compounds such as flavonoids, steroids, triterpenes, coumarins, sesquiterpene lactones, tannins, alkaloids, and carbohydrates, which have demonstrated diverse biological activities, including antioxidant activity [7][8] . These phytochemicals can be recovered from plants through different methods of extraction which are classified in traditional and nonconventional methods 9 . Some traditional methods are decoction, maceration, filtration, and Soxhlet extraction. Among these methods, the decoction is an extraction procedure famous for being used in herbal medicines for the recovering of watersoluble and thermostable metabolites with several biological activities including antioxidant activity. In this procedure, the phytochemicals are extracted from plant material dissolved in an aqueous solution in which heat is applied until boiling point. On the other hand, the non-conventional methods involve other procedures such as the ultrasound and microwave-assisted extraction, and supercritical fluid extraction 10 . Among the non-conventional

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Section: Resultsmentioning

confidence: 99%

Phytochemical and Biological Characterization of Aqueous and Ethanolic Extracts of Parthenium hysterophorus

Jiménez¹,

Zugasti–Cruz²,

Belmares³

et al. 2021

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“…Modern techniques of extraction have emerged from conventional methods to accelerate the extraction process of phytochemicals [27] . In this review, conventional and modern extraction techniques for V. negundo Linn are comprehensively studied.…”

Section: Analytical Methods For Extraction Of Common Phytochemicals Present In Vitex Negundo Linnmentioning

confidence: 99%

Specific analytical methods for the extraction of common phytochemical constituents of Vitex negundo Linn: A mini-review

Atienza¹,

Segui²,

Arcigal³

et al. 2021

J Pharmacogn Phytochem

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Analytical chemistry is a branch of science that deals with chemical analysis responding to changing conditions. It is related to phytochemistry that deals with plant phytochemicals which are beneficial for preventing illnesses and maintaining long-term health. This review comprehensively studied the plant Vitex negundo Linn (Lagundi) by exploring different analytical methods utilized in extracting its common active secondary metabolites depending on its efficiency, selectivity and convenience. In this paper, most studies that had been gathered employed high yield, efficiency, and selectivity, making it difficult to determine a standardized method for V. negundo Linn due to the optimal environmental conditions and solvents that are being utilized. Hence, optimization remains vital for the variety of applications in extracting bioactive compounds from V. negundo Linn to achieve a minimum level of impurities and an acceptable degree of reproducibility and repeatability of bioactive compounds of interest.

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“…Currently, advanced analytical tools and techniques have helped to further fractionate, isolate, and purify compounds from plants, and their biological activities are evaluated. Analytical techniques that have been employed include chromatographic techniques (LC, HPLC, and TLC) and spectra techniques (mass spectrometry, infrared, ultraviolet/visible, nuclear magnetic resonance, X-ray) [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Bioassay-Guided Fractionation, ESI-MS Scan, Phytochemical Screening, and Antiplasmodial Activity of Afzelia africana

Vigbedor

Osei-Owusu²,

Kwakye

et al. 2022

Biochemistry Research International

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Background. Afzelia africana is a plant species with reported numerous medicinal potentials and secondary metabolites. Various parts of the plant have been applied for the treatment of hernia, rheumatism, pain, lumbago, malaria, etc. The study seeks to evaluate the phytochemical constituents, antiplasmodial, and ESI-MS scan of bioassay-guided fractions from the methanol extract of the bark of the plant. Aims. The main aim of the study was to carry out bioassay-guided fractionation of the crude methanol extract of Afzelia africana in order to isolate fractions and to evaluate their antiplasmodial activities and ESI-MS fingerprints. Methods. The methods employed include column chromatographic fractionation, phytochemical screening, antiplasmodial activity (malaria SYBER green assay (MSF)), and ESI-MS profile (full ESI-MS scan). Results. The column chromatographic fractionation and phytochemical screening of the plant led to the separation of the following four fractions: 1 (flavonoids, phenolics, glycosides, terpenoids, and steroids), 2 (alkaloids, anthraquinones, flavonoids, phenolics, glycosides, terpenoids, and steroids), 3 (anthraquinones, flavonoids, phenolics, glycosides, terpenoids, and steroids), and 4 (alkaloids, flavonoids, phenolics, glycosides, terpenoids, and steroids). The antiplasmodial activities of the fractions were tested against the 3D7 strain of Plasmodium falciparum with reported stronger activities for 1 (IC50: 0.097 ± 0.034 μg/mL) and 3 (IC50: 1.43 ± 0.072 μg/mL), and weaker activities for 2 (IC50: >100 μg/mL) and 4 (IC50: 37.09 ± 6.14 μg/mL). The full ESI-MS fingerprint of fractions 1, 2, 3, and 4 revealed the presence of 14, 24, 34, and 37 major molecular ions or compounds in each fraction, respectively.

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Analytical Methods of Isolation and Identification

Cited by 26 publications

References 28 publications

Phytochemical and Biological Characterization of Aqueous and Ethanolic Extracts of Parthenium hysterophorus

Phytochemical and Biological Characterization of Aqueous and Ethanolic Extracts of Parthenium hysterophorus

Specific analytical methods for the extraction of common phytochemical constituents of Vitex negundo Linn: A mini-review

Bioassay-Guided Fractionation, ESI-MS Scan, Phytochemical Screening, and Antiplasmodial Activity of Afzelia africana

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