Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex tablets

Abstract

Comtrex ® tablets composed of paracetamol, pseudoephedrine and brompheniramine are widely used for relieving symptoms related to common cold. This study has overcome the challenging dosage form ratio (250:15:1) and proposed chromatographic methods for analyzing the ternary combination were utilized displaying different apparatus, solvents and sensitivity ranges. Three chromatographic methods namely thin layer chromatography (TLC), high performance liquid chromatography with ultra-violet detection (HPLC–UV) and ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were developed and validated for the simultaneous determination of the three drugs. Concerning the TLC method, aluminum TLC plates pre-coated with silica gel 60F 254 were used and methanol:water:ammonia (9:1:0.1, v/v/v) was applied as a mobile phase; scanning of the plates was carried out at 254 nm. For the HPLC–UV method C 18 column was used with an isocratic elution mobile phase composed of water:acetonitrile (75:25, v/v; pH 3.2) and the detection was at 210 nm. For the UPLC-MS/MS method; separation was performed on a UPLC-BEH C 18 column with methanol: 0.1% ammonium formate (60:40, v/v) as the mobile phase utilizing diphenhydramine as an internal standard and mass spectrometry was used for detection. The methods were simple, sensitive, accurate and precise. Statistical analysis revealed no significant difference from the reported methods in regard to accuracy and precision. Electronic supplementary material The online version of this article (10.1186/s13065-019-0595-6) contains supplementary material, which is available to authorized users.

Sensitive and selective determination of paracetamol in antipyretic children's syrup with a polyglycine modified glassy carbon electrode

“…5 According to articles that have been published until now there are different advanced, rened and sensitive methods that have been applied for the determination of ACOP i.e., titrimetric, [13][14][15] colorimetry, [16][17][18][19] spectrophotometry and spectroscopy, [20][21][22][23] gas chromatography, [24][25][26][27][28] near-infrared transmittance spectroscopy, [29][30][31][32] high performance liquid chromatography (HPLC), [33][34][35] chemiluminescence, 36-39 uorescence, [40][41][42][43] liquid chromatography tandem mass spectrometry, [44][45][46] capillary electrophoresis 47,48 and thin-layer chromatography (TLC). 49,50 In general, these methods are not suitable for routine analysis of ACOP because they require expensive instruments or samples require pretreatment steps before analysis. 51 On the other hand, in analytical research, qualities such as low cost, ease of execution, responsiveness, reproducibility, consistency and rapid determination of drug components are of paramount importance, as are quality control and medical laboratories.…”

“…, titrimetric, 13–15 colorimetry, 16–19 spectrophotometry and spectroscopy, 20–23 gas chromatography, 24–28 near-infrared transmittance spectroscopy, 29–32 high performance liquid chromatography (HPLC), 33–35 chemiluminescence, 36–39 fluorescence, 40–43 liquid chromatography tandem mass spectrometry, 44–46 capillary electrophoresis 47,48 and thin-layer chromatography (TLC). 49,50…”

Poly (Orange CD) sensor for paracetamol in presence of folic acid and dopamine

“…Therefore, developing a simple, fast response, economical, sensitive, accurate, and reliable detection method for the assessment of PA is highly demanded in the medical field. There are lots of methods like capillary electrophoresis 11 , titrimetry 12 , SEC, LC–MS, HPLC 13 – 15 , chemiluminescence 16 , spectrofluorimetric 17 , and spectrophotometry 18 – 20 which have been availed for the assessment of PA. Among all these methods, the electrochemical method stands out with its simplicity, sensitivity, selectivity, modest and fast response.…”

Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen

“…Therefore, there is growing research on the simultaneous analysis of drug mixtures using chromatography. HPLC methods have been used for the quantitative and qualitative analysis of NSAIDs [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]. Isocratic methods are used to determine one or two compounds, while gradient methods are used for multiple drugs [26].…”