Analytical methods for detection of human cytomegalovirus clinched biosensor a cutting-edge diagnostic tool

Hasan, Mohd. Rahil; Sharma, Pramod; Anzar, Nigar; Pundir, C.S.; Pilloton, Roberto; Narang, Jagriti; Shetti, Nagaraj P.

doi:10.1016/j.bea.2021.100006

Cited by 13 publications

(3 citation statements)

References 57 publications

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“…Human cytomegalovirus (HCMV), also known as a human herpesvirus‐5 (HHV‐5), is a common cause of infections in approximately 60% of the population in developed countries and more than 90% in developing countries [ 1 , 2 , 3 ]. HCMV contains a double‐strand DNA genome wrapped with a protein complex called a capsid [ 4 , 5 ]. Capsid and DNA are covered with a protein layer that is further covered by a lipid bilayer, known as envelop [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Molecular techniques are the most widely used approaches for diagnosing the HCMV. Among the molecular techniques, PCR‐based methods are considered the gold standard [ 4 , 10 , 11 , 12 ]. However, PCR‐based methods suffer from several drawbacks that hamper their use such as generating misleading results, the need for adequately trained personnel and complicated DNA extraction process [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Capsid and DNA are covered with a protein layer that is further covered by a lipid bilayer, known as envelop [6]. The interaction between the virus and its host cell is facilitated by various viral glycoproteins embedded within the envelop [4,7]. HCMV infection is usually asymptomatic, but in some cases, it can be a serious and life-threatening infection, for example, in immune-compromised individuals, and may cause childhood disability due to in utero HCMV infection (5).…”

Section: Introductionmentioning

confidence: 99%

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Microelectromechanical system‐based biosensor for label‐free detection of human cytomegalovirus

Alzahrani

Assaifan

Al‐Gawati

et al. 2022

IET Nanobiotechnology

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The human cytomegalovirus (HCMV) is an asymptomatic common virus that is typically harmless, but in some cases, it can be life threatening. Thus, there is an urgent need to develop novel diagnostic methods and strengthen the efforts to combat this virus. A microcantilever-based biosensor functionalised with the UL83-antibody of HCMV (UL83-HCMV antibody) has been developed to detect the UL83-antigen of HCMV (UL83-HCMV antigen) at different concentrations ranging from 0.3 to 300 ng/ml. The response of the biosensor to the presence of UL83-HCMV antigen was measured through the shift in resonance frequency before and after antigen-antibody binding. The system shows a low detection limit of 84 pg/ml, which is comparable to traditional sensors, and a detection time of less than 15 min was achieved. The selectivity of the sensor was demonstrated using three different proteins with and without the UL83-HCMV antigen. The biosensor shows high selectivity for the UL83-HCMV antigen. Mass loading by the UL83-HCMV antigen was roughly estimated with a sensitivity of ~30 fg/Hz. This technique is crucial for the fabrication of portable and low-cost biosensors that can be used in real-time monitoring and enables early medical diagnosis.

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