Analytical Methods for Allergen Control in Food Processing

Lee, Nanju Alice; Lopata, Andreas L.; Colgrave, Michelle L.

doi:10.3390/foods12071439

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Allergenic substances are largely proteins. PCR methods based on the detection of specific DNA sequences encoding proteins are appreciated worldwide because of the minor effect that food processing has on DNA compared with the effect of processing on the expressed protein [16,26,27].…”

Section: Discussionmentioning

confidence: 99%

Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food

Tramuta,

Decastelli,

Ingravalle

et al. 2024

Foods

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In accordance with U.S. FDA Foods Program Regulatory Science Steering Committee guidelines, with this study, we optimized and validated a commercial real-time PCR method for the detection of low amounts of lupin in four classes of food matrices: chocolate cookies, ragù, Olivier salad, and barley and rice flour. DNA extracted from blank (true negative) samples artificially contaminated with lupin (Lupinus albus) flour at 1000 ppm underwent dilutions with the DNA extracted from the true negative samples up to 0.5 ppm. The limit of detection for real-time PCR was 0.5 ppm in the complex matrices (range, Ct 26–34), making this a specific, robust, and rapid method for lupin allergen detection and labeling. Our validation data support the suitability of this commercially available real-time PCR method for this purpose.

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Section: Discussionmentioning

confidence: 99%

Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food

Tramuta,

Decastelli,

Ingravalle

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…For instance, the European Union necessitates labeling of 14 items, including eggs, milk, and peanuts, whereas the USA and Japan mandate labeling of 8 and 7 items, respectively [5,6]. Enzymelinked immunosorbent assay (ELISA), being recognized as a powerful tool, is considered the gold standard in many countries, including the aforementioned ones, for food allergen detection [5,7,8]. Lateral flow immunoassay (LFI) is widely employed as a simple allergen detection method [9,10].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Epitope Analysis of Monoclonal Antibodies Binding to Hen Egg Ovalbumin Using a Peptide Array

Terada,

Akimoto,

Sakoda

et al. 2024

Foods

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Food allergies are a significant health issue worldwide. In many countries, labeling of primary allergens in food products has been made mandatory to ensure consumer safety. In food manufacturing settings, the lateral flow immunoassay (LFI)—based on antigen–antibody reactions—is a rapid and accurate method for allergen testing and is widely used. Peptide arrays are tools that enable the synthesis of peptides of any sequence on a substrate and high-throughput analysis of their interactions with chemicals. This study aimed to investigate a new application of peptide arrays in the field of food technology, particularly in the development of antibodies for food allergen testing. First, monoclonal antibodies against hen egg ovalbumin, a major food allergen, were produced. Then, using a peptide array, the epitope and specificity of the antibodies were comprehensively and precisely analyzed. Finally, an LFI kit incorporating the antibodies demonstrated both high specificity and detection sensitivity for food allergen testing. These findings indicate that peptide arrays are valuable tools in the development of antibodies for food allergen testing, ensuring reliability and accuracy at the molecular level.

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Analytical Methods for Allergen Control in Food Processing

Abstract: Food allergy and food-related anaphylaxis have become a growing public health and food safety issue worldwide [...]

Cited by 2 publications

References 22 publications

Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food

Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food

Comprehensive Epitope Analysis of Monoclonal Antibodies Binding to Hen Egg Ovalbumin Using a Peptide Array

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