Analytical methodologies used for the determination of colistin in biological fluids. Is it still a challenge?

Dagla, Ioanna; Karkoula, Evangelia; Baira, Eirini; Tsarbopoulos, Anthony; Gikas, Evagelos

doi:10.1016/j.jpba.2018.11.044

Cited by 15 publications

(12 citation statements)

References 51 publications

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“…Another source of antibiotics in WWTPs are veterinary medicines (Kemper 2008 ). Under this application, colistin (CST) is a polymyxin antibiotic that is widely used in animal farms to treat Gram-negative infections (Liu et al 2016 ; Kempf et al 2016 ) and it has re-emerged as a ‘last-resort’ antibiotic to target multidrug-resistant infections (Dagla et al 2019 ). The occurrence of CST remains limited given that analytical methods for its quantification remain under development for environmental samples (Song et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of CST remains limited given that analytical methods for its quantification remain under development for environmental samples (Song et al 2020 ). However, the detection of CST on biological matrices (Dagla et al 2019 ) and the presence of a CST resistome in bacteria from WWTPs (Hembach et al 2017 ) suggest that CST may pose a risk to microbial communities.…”

Section: Introductionmentioning

confidence: 99%

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Pharmaceuticals and personal care products’ (PPCPs) impact on enriched nitrifying cultures

Lopez

Nnorom

Tsang

et al. 2021

Environ Sci Pollut Res

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The impact of pharmaceutical and personal care products (PPCPs) on the performance of biological wastewater treatment plants (WWTPs) has been widely studied using whole-community approaches. These contaminants affect the capacity of microbial communities to transform nutrients; however, most have neither honed their examination on the nitrifying communities directly nor considered the impact on individual populations. In this study, six PPCPs commonly found in WWTPs, including a stimulant (caffeine), an antimicrobial agent (triclosan), an insect repellent ingredient (N,N-diethyl-m-toluamide (DEET)) and antibiotics (ampicillin, colistin and ofloxacin), were selected to assess their short-term toxic effect on enriched nitrifying cultures: Nitrosomonas sp. and Nitrobacter sp. The results showed that triclosan exhibited the greatest inhibition on nitrification with EC50 of 89.1 μg L−1. From the selected antibiotics, colistin significantly affected the overall nitrification with the lowest EC50 of 1 mg L−1, and a more pronounced inhibitory effect on ammonia-oxidizing bacteria (AOB) compared to nitrite-oxidizing bacteria (NOB). The EC50 of ampicillin and ofloxacin was 23.7 and 12.7 mg L−1, respectively. Additionally, experimental data suggested that nitrifying bacteria were insensitive to the presence of caffeine. In the case of DEET, moderate inhibition of nitrification (<40%) was observed at 10 mg L−1. These findings contribute to the understanding of the response of nitrifying communities in presence of PPCPs, which play an essential role in biological nitrification in WWTPs. Knowing specific community responses helps develop mitigation measures to improve system resilience.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pharmaceuticals and personal care products’ (PPCPs) impact on enriched nitrifying cultures

Lopez

Nnorom

Tsang

et al. 2021

Environ Sci Pollut Res

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“…Various methods have been described for the quantification of colistin and CMS in biological matrices 9 . However, these published methods describe mostly analysis in plasma 10–14 and urine, 12–14 and can not be directly applied to the matrices in the present study.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry

Gijsen

Brantegem

et al. 2020

Drug Testing and Analysis

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Colistin (polymyxin E) is a polycation antibiotic which is increasingly used (administered as colistin methanesulfonate, CMS) as a salvage therapy in critically ill patients with multidrug resistant Gram‐negative infections. Even though colistin has been used for more than 50 years, its metabolic fate is poorly understood. One of the current challenges for studying the pharmacokinetics (PK) is the precise and accurate determination of colistin in in vitro and in vivo studies. In the present study, we developed and validated a series of sensitive and robust liquid chromatography tandem mass spectrometry (LC–MS/MS) methods for analysing biological samples obtained from in vitro and in vivo disposition assays. After a zinc acetate‐mediated precipitation, hydrophilic–lipophilic‐balanced solid phase extraction (HLB‐SPE) was used for the extraction of colistin. The compounds were retained on a hydrophilic interaction liquid chromatography (HILIC) column and were detected by MS/MS. CMS was quantified by determining the produced amount of colistin during acidic hydrolysis. The developed methods are sensitive with lower limits of quantification varying between 0.009 μg/mL and 0.071 μg/mL for colistin A, and 0.002 μg/mL to 0.013 μg/mL for colistin B. The intra‐ and inter‐day precision and accuracy were within ±15%. Calibration curves of colistin were linear (0.063 μg/mL to 8.00 μg/mL) within clinically relevant concentration ranges. Zinc acetate‐mediated precipitation and the use of a HILIC column were found to be essential. The developed methods are sensitive, accurate, precise, highly efficient and allow monitoring colistin and CMS in biological samples without the need for an internal standard.

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“…Although restricted by WHO (Robertson et al 2019), the presence of OFX continues in raw sewage and e uent in WWTPs (Brown et al 2006;Dinh et al 2017), reaching concentrations of 7.9 µg L − 1 in Asia (Leung et al 2012;Minh et al 2009) and 8.6 µg L − 1 in Europe (Dinh et al 2017) Another source of antibiotics in WWTPs are veterinary medicines (Kemper 2008). Under this application, colistin (CST) is a polymyxin antibiotic that is widely used in animal farms to treat Gram-negative infections (Liu et al 2016;Kempf et al 2016) and it has re-emerged as a "last-resort" antibiotic to target multidrug-resistant infections (Dagla et al 2019). The occurrence of CST remains limited given that analytical methods for its quanti cation remain under development for environmental samples (Song et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of CST remains limited given that analytical methods for its quanti cation remain under development for environmental samples (Song et al 2020). However, the detection of CST on biological matrices (Dagla et al 2019) and the presence of a CST resistome in bacteria from WWTPs (Hembach et al 2017) suggest that CST may pose a risk to microbial communities.…”

Section: Introductionmentioning

confidence: 99%

Pharmaceuticals and Personal Care Products (PPCPs) Impact Enriched Nitrifying Cultures

Lopez

Nnorom

Tsang

et al. 2021

Preprint

View full text Add to dashboard Cite

The impact of pharmaceutical and personal care products (PPCPs) on the performance of biological wastewater treatment plants (WWTPs) has been widely studied using whole-community approaches. These contaminants affect the capacity of microbial communities to transform nutrients; however, most have neither honed their examination on the nitrifying communities directly nor considered the impact on individual populations. In this study, six PPCPs commonly found in WWTPs, including a stimulant (caffeine), an antimicrobial agent (triclosan), an insect repellent ingredient (N,N-diethyl-m-toluamide (DEET)), and antibiotics (ampicillin, colistin, and ofloxacin), were selected to assess their short-term toxic effect on enriched nitrifying cultures: Nitrosomonas sp. and Nitrobacter sp. The results showed that triclosan exhibited the greatest inhibition on nitrification with EC50 of 89.1 µg L− 1. From the selected antibiotics, colistin significantly affected the overall nitrification with the lowest EC50 of 1 mg L− 1, and a more pronounced inhibitory effect on ammonia-oxidizing bacteria (AOB) compared to nitrite-oxidizing bacteria (NOB). The EC50 of ampicillin and ofloxacin were 22 and 12.7 mg L− 1, respectively. Additionally, experimental data suggested that nitrifying bacteria were insensitive to the presence of caffeine. In the case of DEET, moderate inhibition of nitrification (< 40%) was observed at the highest concentration tested. These findings contribute to the understanding of the response of nitrifying communities in presence of PPCPs, which play an essential role in biological nitrification in WWTPs. Knowing specific community responses helps develop mitigation measures to improve system resilience.

show abstract

Analytical methodologies used for the determination of colistin in biological fluids. Is it still a challenge?

Cited by 15 publications

References 51 publications

Pharmaceuticals and personal care products’ (PPCPs) impact on enriched nitrifying cultures

Pharmaceuticals and personal care products’ (PPCPs) impact on enriched nitrifying cultures

Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry

Pharmaceuticals and Personal Care Products (PPCPs) Impact Enriched Nitrifying Cultures

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