Analytical isotachophoresis in the analysis of synthetic peptides

Kopwillem, Ann; Moberg, Ulla; Westin-Sjödahl, Gertrud; Lundin, Ronny; Sievertsson, Hans

doi:10.1016/0003-2697(75)90284-5

Cited by 27 publications

(1 citation statement)

References 11 publications

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“…Capillary tube isotachophoresis was used for quantitation of the amino acids glycine, L-alanine, and Lvaline (17). A capillary tube isotachophoretic analyzer IP-2A (Shimadzu Seisakusho Ltd., Kyoto, Japan) equipped with a potential gradient detector was employed.…”

mentioning

confidence: 99%

Betaine: New Oxidant in the Stickland Reaction and Methanogenesis from Betaine and l -Alanine by a Clostridium sporogenes-Methanosarcina barkeri Coculture

Naumann

Hippe

Gottschalk

1983

Appl Environ Microbiol

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Growing and nongrowing cells of Clostridium sporogenes fermented betaine with l -alanine, l -valine, l -leucine, and l -isoleucine as electron donors in a coupled oxidation-reduction reaction (Stickland reaction). For the substrate combinations betaine and l -alanine and betaine and l -valine balance studies were performed; the results were in agreement with the following fermentation equation: 1 R- CH(NH 2 )-COOH + 2 betaine + 2 H 2 O → 1 R-COOH + 1 CO 2 + 1 NH 3 + 2 trimethylamine + 2 acetate. Growth and production of trimethylamine were strictly dependent on the presence of selenite in the medium. With cell suspensions it was shown that C. sporogenes was unable to catabolize betaine as a single substrate. Betaine, however, was reduced to trimethylamine and acetate under an atmosphere of molecular hydrogen. For the reduction of betaine by cell extracts of C. sporogenes , dimercaptans such as 1,4-dithiothreitol could serve as electron donors. No betaine reductase activity was detected in cells grown in a complex medium without betaine. The pH optimum of betaine reductase was at pH 7.3. When C. sporogenes was cocultured with Methanosarcina barkeri strain Fusaro on betaine together with l -alanine, an almost complete conversion of the two substrates to CH 4 , NH 3 , and presumably CO 2 was observed.

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confidence: 99%

Betaine: New Oxidant in the Stickland Reaction and Methanogenesis from Betaine and l -Alanine by a Clostridium sporogenes-Methanosarcina barkeri Coculture

Naumann

Hippe

Gottschalk

1983

Appl Environ Microbiol

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Isotachophoretic analysis of the end‐product and intermediates in the synthesis of the C‐terminal pentapeptide of bombinin

Friedel¹,

Holloway²

1981

Electrophoresis

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Capillary isotachophoresis has been applied as an analytical monitor during the synthesis of the C‐terminal pentapeptide, Gln‐His‐Phe‐Ala‐Asn, of bombinin. The samples were separated as cations since the C‐terminal carboxyl group was modified to an uncharged amide. Potassium and beeta‐alanine were employed as leading and terminating ions and acetate was the common counter‐ion. The analyses demonstrated that the stages of synthesis up to the tripeptide, Phe‐Ala‐Asn, gave quite pure products, whereas a considerable amount of impurity was detectable after the introduction of His into the molecule. From the peptide chemist's point of view, this amino acid presents special problems. However, the impurities found in the isotachopherograms were not detectable by conventional methods such as thin‐lyer chromatography. The rapid and sensitive method of isotachophoresis should prove useful to the peptide chemist for assessment of his products, and for redesign or modification of non‐optimal synthetic strategies.

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The analysis of amino acids and peptides by isotachophoresis

Holloway

Pingoud

1981

Electrophoresis

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Analytical isotachophoresis in the analysis of synthetic peptides

Cited by 27 publications

References 11 publications

Betaine: New Oxidant in the Stickland Reaction and Methanogenesis from Betaine and l -Alanine by a Clostridium sporogenes-Methanosarcina barkeri Coculture

Betaine: New Oxidant in the Stickland Reaction and Methanogenesis from Betaine and l -Alanine by a Clostridium sporogenes-Methanosarcina barkeri Coculture

Isotachophoretic analysis of the end‐product and intermediates in the synthesis of the C‐terminal pentapeptide of bombinin

The analysis of amino acids and peptides by isotachophoresis

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