Analytical Identity of an Inhibitor of Sodium Transport Isolated from Human Serum and Urine

Spustová, Viera; Geryková, M; Lichardus, B; Dzúrik, R

doi:10.1159/000183330

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Its release from the hypothalamus can be detected by the increase in amount of extracellular fluid such as that which occurs in chronic uremics, water immersion, primary hyperalodosteronism and high salt loading. This OLF acts on the renal collecting ducts and tubules by inhibiting Na reabsorption, and its action causes a strongly enhanced natriuresis and diuresis 24 – 29 ) .…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte Sodium Transport in Dialyzed Uremic Patients

Yoon

Kim

Koo

et al. 1989

Korean J Intern Med

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To investigate the status of the Na+ concentration and ionic fluxes in red cells of human subjects with dialyzed chronic uremia, the authors measured the Na+-K+ pump activity as well as Na+-K+ cotransport (CoT), Na+-Li+ countertransport (CTT) and Na+ passive permeability in erythrocytes from 37 normal subjects and 23 chronic uremic patiens receiving maintenance hemodialysis. The mean intracellular Na+ concentration [Na+]i value in the pre-dialytic group was significantly lower than that in control subjects (p<.0001), but tended to recover to the normal value of [Na+]i in the post-dialytic group. The mean intracellular K+ concentration value in the post-dialytic group was significantly higher than that of the control group (p<.001), but not significantly different from that of the pre-dialytic group. It was found that the Na+-K+ pump activity of erythrocytes in the pre- and post-dialytic groups markedly decreased over that of the normal control group with statistical significance (p<.0001, respectively). The Na+-K+ pump activity in the post-dialytic group, however, tended to recover, but not significantly. The rate constant for ouabain-sensitive Na+ efflux in the post-dialytic group was significantly decreased over that of the normal controls (p<.05). The authors observed a significant decrease of the Na+ CoT value (p<.001 respectively) and rate constant for Na+ CoT (p<.05, respectively) in the patients with pre- and post-dialytic uremia vs. that of normal subjects. Also, the authors observed a marked decrease of the Na+-Li+ CTT value in the patients in the pre- and post-dialytic groups than that of the control subjects (p<.05, respectively). Passive Na+ permeability in the patients with pre-dialytic uremia was decreased markedly compared to the normal subjects (p<10−5), but its value in the post-dialytic group tended to recover to the normal value. In conclusion, our studies demonstrate that another possible mechanism of inhibition of the Na+-K+ pump in pre- dialytic uremia might then be a secondary adaptive response of the cell to maintain normal intracellular ion concentration and transmembrane ion gradients in the face of the reduced [Na+]i due to decreased passive Na+ permeability.

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Section: Discussionmentioning

confidence: 99%

Erythrocyte Sodium Transport in Dialyzed Uremic Patients

Yoon

Kim

Koo

et al. 1989

Korean J Intern Med

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“…It’s release can be detected by the increase in amount of extracllular fluid such as that which occurs in chronic uremics. 31 , 32) This OLF acts on the renal collecting ducts and tubules and, by inhibiting Na reabsorbtion, its action cause a strongly enhanced Na excretion and diuresis. However, on the other hand, OLF inhibits the efflux of Na from the cell membrane by acting on the erythrocytes, leukocytes, platelets, nerve cells and smooth muscle cells of the vessel.…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte Na+-K+ ATPase Activity in the Genesis of Reducing Renal Mass in Hypertensive Rats

Yoon

Bang

Min

et al. 1986

Korean J Intern Med

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To study the relationship between natriuretic factor and experimental low renin hypertension, the erythrocyte Na+-K+ ATPase activities in the genesis of reducing renal mass in hypertensive rats were measured. The results were as follows : The fractional excretion of Na in hypertensive, reduced renal mass rats (saline-drinking) was higher than that of normotensive, reduced renal mass rats (water-drinking) (p<.001). The plasma renin activities in the hypertensive group were markedly lower than those of the normotensive group (p<.001).The total ATPase activities of rat erythrocyte membrane in the hypertensive group were lower than in those of the normal and the normotensive groups (p<.001, p<.02).The Mg++-ATPase activities of rat erythrocyte membrane in the normotensive group tended to decrease significantly (p<.001), but the differences between the normotensive and hypertensive group were not significant.The Na+-K+ ATPase activities of rat erythrocyte membrane in the hypertensive group were lower than those of the normal and the normotensive group (p<.001). However, the difference between the normal and the normotensive groups was not significant.When erythrocyte membrane taken from normal rats was incubated with supernates of boiled plasma from normotensive rats, the total ATPase activities of erythrocyte membrane were not different from those of hypertensive rats.When erythrocyte membrane taken from normal rats was incubated with supernates of boiled plasma from normotensive rats, the Mg++ ATPase activities of erythrocyte membrane were lower than those of hypertensive rats (p<.01).When erythrocyte membrane taken from normal rats was incubated with supernates of boiled plasma from hypertensive rats, the Na+-K+ ATPase activities of erythrocyte membrane were lower than those of normotensive rats (p<.01).Based on the above findings, it is suggested that the pathogenesis of low renin, reduced renal mass hypertension is primarily mediated by a natriuretic factor (or an ouabain-like factor, inhibitor of Na+-K+ ATPase) produced by extracellular fluid volume expansion.

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Analytical Identity of an Inhibitor of Sodium Transport Isolated from Human Serum and Urine

Cited by 2 publications

References 7 publications

Erythrocyte Sodium Transport in Dialyzed Uremic Patients

Erythrocyte Sodium Transport in Dialyzed Uremic Patients

Erythrocyte Na+-K+ ATPase Activity in the Genesis of Reducing Renal Mass in Hypertensive Rats

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