Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography

Rouser, George; Galli, Cláudio; Lieber, Ellen R.; Blank, Merle L.; Privett, O. S.

doi:10.1007/bf02663969

Cited by 211 publications

(24 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(modified from Mangold, 1961); solvent B, petroleum ether +diethy1 ether, 9: I (by vol.). Either chromic acid (Rouser, Galli, Lieber, Blank & Privett, 1964) or fluorescent reagent (Neudoerffer & Lea, 1966) was used to detect lipids. Samples to be analysed by gas-liquid chromatography (GLC) were inter-esterified with sodium methylate (de Man, I 964) and resolved on columns containing polyethylene glycol adipate (PEGA) or methyl silicone gum E3o (E3o) according to the procedures of Neudoerffer & Lea (I 966).…”

mentioning

confidence: 99%

Occurrence of Waxes in Acinetobacter

Gallagher¹

1971

Journal of General Microbiology

View full text Add to dashboard Cite

During a study of the effects of environmental temperatures on the composition of the fats in three strains of Acinetobacter (Gallagher, 1968) an unusual component was detected when lipid extracts were analysed by thin-layer chromatography. This component was shown to consist of a mixture of waxes. As waxes occur rarely in bacteria it was of interest to characterize them and to study the effect of incubation temperature on their composition. METHODS

show abstract

mentioning

confidence: 99%

Occurrence of Waxes in Acinetobacter

Gallagher¹

1971

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…Brain PE (Pierce Chemical Co.) was purified on silicic acid columns [9]. The purified fraction gave one spot on TLC (Silica gel G) using three different solvent systems: RF=0.30 in chloroformmethanol-water (65: 25:4, v/v/v); RF = 0.23 in chloroform-methanol-35 % ammonia (14: 6:1, v/v/v) [6]; and R F =0.44 in n-butanol-acetic acid-water (3 : 1 : 1, v/v/v) [8]. Bovine brain CEB (Pierce Chemical Co.) showed two spots on TLC, which is typical of all CEB reported by other investigators [6].…”

Section: Methodsmentioning

confidence: 99%

The effect of electric fields on brain lipid-cholesterol films

1973

View full text Add to dashboard Cite

Summary. The effect of a direct-current electric field on films of brain cerebroside and cholesterol and mixtures with brain phosphatidylethanolamine (PE) using infrared spectral techniques was studied. The intensities of the spectral bands assigned to the vibration of the phosphate and the -(CH2),-grou p of the fatty acid chain of the phospholipid increased to a maximum as the applied voltage increased. The changes were similar to those previously observed with films of the phospholipid, but higher voltages were required to reach the maximum than with the phospholipid films. The spectral changes are related to conformational changes occurring in the phospholipid component of the films containing cerebroside and cholesterol. The infrared spectra of the mixtures indicate that there is hydrogen bonding between the phosphatidylethanolamine and cholesterol in the films and that the phospholipid exists in the nonpolar form in the films. As found previously with films of brain cephalin and lecithin, the electric field strength at which the maximum intensities of spectral bands are observed varies inversely with the thickness.During the excitation of the neuron, changes occur in the permeability of the membrane to ions. These changes may be associated with alterations in the molecular configuration of the membranal constituents some of which are lipids. The behavior of films of lipids may provide information concerning the changes that occur in these substances in the neuronal membrane during excitation. Films are used because they can be considered as models for the lipid structures of the membrane.Previous observations on the effect of direct-current electric fields on films of the brain phospholipids, cephalin and lecithin, have shown that the intensities of certain infrared absorption bands were altered [7]. Since the phospholipids exist in the membrane in combination with cerebroside (CEB) and cholesterol (CHOL), the effect of the interaction between these lipids could be determined by performing similar spectral measurements with mixtures of the lipids and CHOL. A mixture of PE-CEB-CHOL with the molar ratio of 1:0.5:1 was selected since this represented the

show abstract

“…The solvent systems used for one-dimensional and two-dimensional TLC for fractions specified are shown in Table I. Spots were made visible by charring (15) and Rhodamine 6G (14) for total lipids. The molybdenum blue reagent (3) was used to detect chloramben in a total volume of 2 ml.…”

mentioning

confidence: 99%

Regulation of Lipid Synthesis in Soybeans by Two Benzoic Acid Herbicides

Muslih¹,

Linscott²

1977

Plant Physiol.

View full text Add to dashboard Cite

The effects of 3-nitro-2,5-dichlorobenzoic acid (dinoben) and 3-amino-2,4-dlchlorobenzolc acid (chloramben) on lipid formation and on the incorporation of various substrates into lipids by intact seeds and subceflular fractions of germinating soybean (Glycine max [L.] Merr.'Amsoy') were studied. Dinoben (20 lg/ml) inhibited synthesis of total lipils 67%, neutral lipids 73%, glycolpids 51%, and pbospholipids 39% in germinating seeds. When polar lipids were analyzed further, inhibition of individel lipid dcass was also observed. Chloramben (20 pg/ml) stimulated total lpid synthesis 25%. With the exception of the mitochondrial fraction where malonate thiokinase was absent, dinoben inhibited up to 99% the incorporation of acetate and malonate into lpids, but did not inhibit acetyl-CoA and malonyl-CoA incorporation.Chloramben stimulated the incorporation of aU substrates tested into lipids by all fractions except the mitochodrial fraction when malonate was the substrate. When dinoben and chloramben were used in combinations, chioramben did not reverse the inhibitory effect of dinoben.It is concluded that the dinoben inhibitory effect specific and is assodated with the acetate and malonate thiokinase systems. The chioramben effect b stimulatory to either acetyl-CoA carboxylase or fatty add synthetase or both.Recently, research on various aspects of soybean metabolism has increased substantially. However, little is known about specific metabolic processes in soybean during germination. In particular, lipid synthesis during germination of soybean has not been studied in great detail.Quantitative and qualitative alteration of membrane lipid composition results in changes in chloroplast and mitochondrial functions and membrane permeability capacity (1, 9). Thus, chemical inhibition of lipid synthesis could ultimately result in loss of photosynthetic and respiratory activity, plant necrosis, and changes in the nutritional balance of seedlings. Mann [L.] Merr. 'Amsoy') were germinated at 25 C in small test tubes (one seed/tube) in 0.5 ml of deionized H20 containing 0.25 ,uCi of 1-14C-acetate and the proper concentration of chloramben or dinoben. After 24 hr (unless otherwise indicated), the germinated seeds were transferred to 95 C deionized H20 for 2 min and then washed three times with 100 ml of water.

show abstract

Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography

Cited by 211 publications

References 6 publications

Occurrence of Waxes in Acinetobacter

Occurrence of Waxes in Acinetobacter

The effect of electric fields on brain lipid-cholesterol films

Regulation of Lipid Synthesis in Soybeans by Two Benzoic Acid Herbicides

Contact Info

Product

Resources

About