Analytical characterization of chitosan nanoparticles for peptide drug delivery applications

Abstract

Chitosan-cyclodextrin hybrid nanoparticles (NPs) were obtained by the ionic gelation process in the presence of glutathione (GSH), chosen as a model drug. NPs were characterized by means of transmission electron microscopy and zeta-potential measurements. Furthermore, a detailed X-ray photoelectron spectroscopy study was carried out in both conventional and depth-profile modes. The combination of controlled ion-erosion experiments and a scrupulous curve-fitting approach allowed for the first time the quantitative study of the GSH in-depth distribution in the NPs. NPs were proven to efficiently encapsulate GSH in their inner cores, thus showing promising perspectives as drug carriers.

Optimised nanoformulation of bromocriptine for direct nose-to-brain delivery: biodistribution, pharmacokinetic and dopamine estimation by ultra-HPLC/mass spectrometry method

“…Nasal mucoadhesive nanoparticles (NPs) can be explored to target drugs to the brain, providing more opportunities for antiparkinsonian agent to enter the brain, thereby reducing its side effects, dose and frequency of dosing [10,11]. Among several NPs prepared from biodegradable polymers, NPs based on chitosan (CS) are desirable as CS is a linear cationic polysaccharide and has been widely considered as a versatile polymer for development of delivery systems due to its biological properties such as biocompatibility, biodegradability, low toxicity, high loading and good delivery ability for hydrophilic molecules [12]. CS has a special feature of being able to adhere to nasal mucosal surface for prolonged periods of time due to mucoadhesive property and improve drug absorption by delaying mucociliary clearance.…”

Role of Chitosan, Carboxy Methyl Cellulos, Polyvinyl Pyrrolidone, Kyron T134 and Primogel to Design the Mouth Disintegrating Telmisartan Tablet With Extended Release Profile

“…At the end we mixed both portions of weighing materials for 06 formulations to homogenize in tumbling cylindrical mixer for 20min and compressed into extended release orodispersible tablets by using ZP 19 station rotary tablet compressing machine. (Ieva et al, 2009) …”

“…The most widely used oldest disintegrant, the starch is provided with a dramatic disintegrating properties and are available as Explotab® and Primogel. (Ieva et al, 2009) It is chemically cross-linked and carboxymethylated potato starch. It is manufactured by cross-linking starch with sodium trimetaphosphate followed by carboxymethylation with sodium monochloroacetate.…”

Protein-Peptide Interactions Revolutionize Drug Development

“…In addition to nasal, pulmonary, transdermal, and parenteral delivery routes using chitosan-based nano-and microparticle carriers, chitosan coated particles or pegylated chitosan particles receive particular interest for the delivery of peptides [83,86]. Use of chitosan-based nano-and microparticles for peptide antigens based on luteinizing hormone-releasing hormone [87], peptide hormone insulin [88], glutathione [89,90], heparin [91], and calcitonin [92] are just a few examples on the application of chitosan in peptide delivery.…”