Analytical Characterization of an Inulin-Type Fructooligosaccharide from Root-Tubers of Asphodelusramosus L

Abstract

Plant-based systems continue to play a pivotal role in healthcare, and their use has been extensively documented. Asphodelus L. is a genus comprising various herbaceous species, known by the trivial name Asphodelus. These plants have been known since antiquity for both food and therapeutic uses, especially for treating several diseases associated with inflammatory and infectious skin disorders. Phytochemical studies revealed the presence of different constituents, mainly anthraquinones, triterpenoids, phenolic acids, and flavonoids. Although extensive literature has been published on these constituents, a paucity of information has been reported regarding the carbohydrate composition, such as fructans and fructan-like derivatives. The extraction of water-soluble neutral polysaccharides is commonly performed using water extraction, at times assisted by microwaves and ultrasounds. Herein, we reported the investigation of the alkaline extraction of root-tubers of Asphodelus ramosus L., analyzing the water-soluble polysaccharides obtained by precipitation from the alkaline extract and its subsequent purification by chromatography. A polysaccharide was isolated by alkaline extraction; the HPTLC study to determine its composition showed fructose as the main monosaccharide. FT-IR analysis showed the presence of an inulin-type structure, and NMR analyses allowed us to conclude that A. ramosus roots contain polysaccharide with an inulin-type fructooligosaccharide with a degree of polymerization of 7–8.

WITHDRAWN: Study on the pharmacokinetics of Polygonatum cyrtonema polysaccharide DPC1 through fluorescence labeling

“…Figure 1K shows the Fourier-transform infrared spectra of the samples. The broad and strong absorbance bands at 3385 cm − 1 in the spectrum are attributed to the O-H stretching vibrations, whereas C-O-C glycosidic bond stretching in the sugar ring produces bands at 1129 and 1028 cm − 1 [20,27]. The DPC1-RhB spectra show the typical peaks of the above polysaccharides.…”

In Vivo Pharmacokinetic Study of Polygonatum cyrtonema Polysaccharide DPC1 after Oral and Intraperitoneal Administration

“…Figure 1 K shows the Fourier-transform infrared spectra of the samples. The broad and strong absorbance bands at 3385 cm −1 in the spectrum are attributed to the O–H stretching vibrations, whereas C–O–C glycosidic bond stretching in the sugar ring produces bands at 1129 and 1028 cm −1 [ 22 , 28 ]. The DPC1-RhB spectra show the typical peaks of the above polysaccharides.…”

HPTLC-based fingerprinting: An alternative approach for fructooligosaccharides metabolism profiling

“…Nonetheless, α -naphthol was found to be more sensitive for fructans detection when compared to aniline on HPTLC analysis ( Benkeblia, 2013 ). Lastly, orcinol is also frequently used for fructan and levan like metabolites detection on regular TLC ( Aramsangtienchai et al, 2020 ; Madia et al, 2021 ). These features settled the election of these three reagents as derivatizing reagents for this study.…”

“…These applications have allowed for the visual distinction of fructans’ plant origin, qualitatively and quantitatively ( Alvarado et al, 2014 ). Some examples include the profiling of apple juice supplemented with agave fructans, inulin, and oligofructose ( González-Herrera et al, 2016 ), root-tubers of Asphodelus ramosus ( Madia et al, 2021 ), fructans in Aloe barbadensis ( Salinas et al, 2016 ), among others. However, sample application and chromatographic development conditions vary a lot among studies making difficult to compare data between them ( Mellado-Mojica and López, 2012 ; Alvarado et al, 2014 ; Pérez-López et al, 2021 ).…”