Analytical aspects of biosensors

Pearson, Jacqueline E.; Gill, Anita; Vadgama, Pankaj

doi:10.1258/0004563001899131

Cited by 108 publications

(55 citation statements)

References 137 publications

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…Moreover, the current measured directly depends on the transfer of reactants to the surface of the WE and the transfer of electrons for the oxidation and reduction of the electrically active species; the CE operates to maintain the WE at a constant potential relative to the RE by gathering any current produced by the electrochemical reaction. 29,30 Because the current is measured, this is an example of an amperometric biosensor.In this parametric study of an electrochemical sensor, an electrochemical detection method is combined with enzymatic amplification and an aptamer that experiences target-induced conformational changes. When an electrochemical sensor is used, the aptamer must be immobilized only on the WE.…”

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confidence: 99%

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A Parametric Design Study of an Electrochemical Sensor

Garcia

Chen

Fang

et al. 2010

JALA: Journal of the Association for Laboratory Automation

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T he development of highly sensitive biosensors for the detection of biomolecules, such as the biomarker interleukin-8 for the early detection of oral cancer, requires optimization of sensor design. To augment the performance of an electrochemical sensor, this study used a microscale, aptamer-based electrochemical sensor for detecting botulinum neurotoxin aptamer hybridization. We first used topedown lithographic processing to define the pattern of the electrodes and then used bottomeup manufacturing to modify the surface molecular properties for reducing nonspecific binding. We systemically examined the effects of the design parameters of an aptamerbased electrochemical sensor. Specifically, five key design parameters were examined: the area of the working electrode (WE), the area of the counter electrode (CE), the separation distance between the WE and CE, the overlap length between the WE and CE, and the aptamer concentration. Through an analysis of the signal and noise generated across variations of the different parameters, the significance of each parameter in sensor performance was determined. In particular, we found that the area of the WE was the only key parameter that influenced the performance of the sensor. The output signal level increased with the area of the WE and the signal-to-noise ratio was about constant in the tested range (i.e., from 0.02 to 4 mm 2 ). ( JALA 2010;15:179-88) INTRODUCTIONElectrochemical biosensors synergize the biological recognition element of a biosensor with an electrochemical transducer and have been used to detect a variety of biological targets, such as uropathogenic bacteria, the hepatitis B virus, and the cholera toxin produced by Vibrio cholerae. 1e4 Amenable to miniaturization, the development of microfabricated electrochemical biosensors has resulted in high sensitivity, portability, improved performance and spatial resolution, low power consumption, and the opportunity for integration with other technologies, such as microfluidics. 5,6 Such advantages enable MicroElectrical-Mechanical Systems (MEMS)-based electrochemical biosensors to identify pathogens present in a variety of mediums and bodily fluids, such as blood, saliva, and air more quickly than previous, laboratory-based detection methods. 7e10 Previous work has been done using an electrochemical biosensor in which a sensing paradigm was tested and developed. 7,11e13 For example, Gau et al. 14 developed a MEMS-based electrochemical biosensor for the amperometric detection of Escherichia coli. The electrochemical detection system resulted from the synergy of microfabricated electrodes, self-assembled monolayers via thiol chemistry, DNA hybridization, and enzyme-mediated signal amplification. 14 Gau's 14 electrochemical biosensor used three microfabricated gold electrodes: a working electrode (WE), a counter electrode (CE), and a reference electrode (RE). Using an identical electrode design, Wei et al. 11 and Wei and Ho 15 detected salivary interleukin-8 (IL-8) mRNA using a hairpin probe and botulinum ...

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

mentioning

confidence: 99%

A Parametric Design Study of an Electrochemical Sensor

Garcia

Chen

Fang

et al. 2010

JALA: Journal of the Association for Laboratory Automation

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“…Methods used for DNA sequence detection in those sensors have been reported to be based on radiochemical, enzymatic, fluorescent, electrochemical, optical, and acoustic wave techniques [71]. Some disadvantages of the optical sensors, however, include the requirement of a separate labeling process and an equipment to stimulate the transducer; they are also highly complex, and thus, entail higher cost in order to conduct an analysis [72]. The DNA sequence attachment on the surface of the sensor is a key to high sensitivity, long life-span, and short response time.…”

Section: Cnt/dna-based Nanosensorsmentioning

confidence: 99%

Dispersions Based on Carbon Nanotubes – Biomolecules Conjugates

Capek¹

2011

Carbon Nanotubes - Growth and Applications

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“…Electrochemical and optical detection, used in immunosensors and bioluminescent sensors, are only some of all the detection systems that were imagined and tested (Mallat et al, 2001;Pearson et al, 2000).…”

Section: Mode Of Detectionmentioning

confidence: 99%

“…The advantage of optical transduction is that the signal is measured in a non-destructive way (Pearson et al, 2000). When intensity is measured, the concentration in analyte is proportional to the quantity of emitted light.…”

Section: Optical Detectionmentioning

confidence: 99%

Biosensors for the Environment

Malandain

Fayolle

Bedouelle

2005

Oil & Gas Science and Technology - Rev. IFP

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Résumé -Biocapteurs pour l'environnement -La présence de polluants d'origine industrielle ou agricole dans l'environnement, et dans les eaux en particulier, est liée à l'utilisation massive de composés tels que les herbicides et pesticides, les solvants chlorés ainsi que les composés des essences qui peuvent être déversés lors du transport et du stockage des carburants. Les composés chimiques concernés sont généralement toxiques pour l'homme et les animaux. Cette toxicité nécessite dans les cas de pollution de suivre l'évolution de ces composés (en particulier en cas de menace sur des captages d'eau potable). Les outils classiques de mesure (CPG, CPG/SM, etc.) sont très efficaces du point de vue de la sensibilité et de la reproductibilité mais leur utilisation nécessite d'effectuer des prélèvements sur site, de transporter les échantillons puis de les analyser au laboratoire. Les délais induits peuvent être rédhibitoires et les coûts ne sont pas négligeables quand de nombreux échantillons sont à analyser sur des périodes de temps longues. C'est pour ces raisons que de nouveaux appareils de mesure, biosenseurs ou biocapteurs, qui profitent de l'avancée des connaissances dans plusieurs domaines scientifiques et dans celui de la biologie en particulier, ont été conçus. Ils permettent la création d'outils analytiques aussi fiables mais plus sélectifs et peu encombrants, qui fournissent des mesures en temps réel sur le site étudié. Abstract -Biosensors for the Environment

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Analytical aspects of biosensors

Abstract: This article was prepared under the auspices of the Analytical Investigations Standing Committee of the Scienti®c Committee of the Association of Clinical Biochemists.

Cited by 108 publications

References 137 publications

A Parametric Design Study of an Electrochemical Sensor

A Parametric Design Study of an Electrochemical Sensor

Dispersions Based on Carbon Nanotubes – Biomolecules Conjugates

Biosensors for the Environment

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