Analytical applications of laser excited fluorimetry of molecules in the condensed phase

Laser fluorometry of fluorescein and riboflavin

“…The unique properties of the nitrogen-laser-pumped dye laser can give rise to substantial gains in improving detection limits (1,2). The previously reported detection limits of fluorescein with a laser source and a monochromator (3)(4)(5) were in the order of 30-100 ppt (ppt = 10"12) and are approximately identical to that of a conventional fluorescence spectrophotometer. Recently, however, the fluorescein detection limit, as determined by laser fluorometry, has been reported to be 2 ppt (1).…”

Development of analytical laser spectroscopy at the French Atomic Energy Commission

“…In this context, the use of lasers as excitation source appeared very interesting as demonstrated by the publications of Nicoló Omenetto with co-workers on laser induced atomic fluorescence in various atomization sources [1][2][3].…”

A simple, portable fluorimeter with a compact, inexpensive nitrogen laser source

“…Although nitrogen lasers (and dye lasers pumped by them) have been used as excitation sources for fluorimetry since at least 1974 (1), they have not found widespread use in analytical molecular fluorimetry except in cases such as lowtemperature Shpol'skii spectrometry, which requires a narrow excitation spectral bandwidth (2, 3) and in the excitation of eluants in high-performance liquid chromatography, HPLC (4, 5), where the ability to focus the excitation beam onto a small volume of analyte is highly desirable. Limits of detection for N2-laser-excited fluorescence detection of HPLC eluants have been quite good, generally in the 1-ng/ mL range (6).…”