Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research

Beekes, Michael; Lasch, Peter; Naumann, Dieter

doi:10.1016/j.vetmic.2007.04.010

Cited by 245 publications

(193 citation statements)

References 56 publications

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“…The combo had the highest percentage of discrimination with 82%, (Beekes et al, 2007;Cai and Singh, 2004;Khaustova et al, 2010;Krimm and Bandekar, 1986;Oberg et al, 2004;Stuart, 1997).…”

Section: Resultsmentioning

confidence: 99%

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

2015

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Introduction: Saliva is the most promising biofluid to monitor the physiological state of athletes, because this method is not invasive and has low contamination risks. The characterization of saliva by Fourier transform infrared spectroscopy (FT-IR) has been studied as an alternative technique to the standard clinical analysis. However, methodological procedures for saliva analysis are not completely clear, especially in terms of influence of storage conditions and sample preparations for infrared analysis. Thawed saliva includes a precipitate, which may influence the infrared spectral analysis. Thus, the purpose of this study was to show the spectral differences of the precipitate, supernatant, and a combo, as well as the best way to classify the physiological state of the athletes by FT-IR. Methods: The saliva collection was performed before, immediately after, and two hours after a handball match. After the storage of samples at -20 ○ C, it was possible to identify two phases (precipitate and supernatant) and to determine the biochemical differences between the spectra of each phase, which were distinctly analyzed by the second derivative and deconvolution bands. Results: The precipitate and supernatant results showed characteristic bands, especially in the protein regions. All FT-IR spectra were also statistically classified by linear discriminant analysis (LDA), using principal component analysis (PCA). The LDA precipitate and supernatant had lower value when compared to combo spectra (Combination of precipitate and supernatant) with 82%, showing that this combination is the best way to discriminate spectra of saliva samples collected before, immediately after, and 2 h after physical effort. Discussion: The results showed that it is possible to differentiate biochemically the two salivary phases, as well as the importance of the homogenization process of saliva samples to classify the physiological status of athletes using FT-IR.

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“…The combo had the highest percentage of discrimination with 82%, (Beekes et al, 2007;Cai and Singh, 2004;Khaustova et al, 2010;Krimm and Bandekar, 1986;Oberg et al, 2004;Stuart, 1997).…”

Section: Resultsmentioning

confidence: 99%

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

2015

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“…This correlation is used in FTIR spectroscopy to assign characteristic frequencies of structure-sensitive amide bands to different types of secondary protein structure, such as ␣-helices or ␤-sheets. There are nine amide bands in the IR spectrum (37). The amide I band used in our study, in which secondary structure elements, such as ␣-helix, ␤-sheet, or turns and loops, absorb IR light at different wavelengths, has been established as the most useful band for the analysis of secondary protein structure (for details, see Refs.…”

Section: Discussionmentioning

confidence: 99%

“…The amide I band used in our study, in which secondary structure elements, such as ␣-helix, ␤-sheet, or turns and loops, absorb IR light at different wavelengths, has been established as the most useful band for the analysis of secondary protein structure (for details, see Refs. [37][38][39].…”

Section: Discussionmentioning

confidence: 99%

“…In this context, it has to be noted that the association between specific IR absorption bands and ␣-helical structure elements in PrP is currently under discussion (38,40). For our study, however, this is not critical because we focused on IR bands that are assigned to ␤-sheets according to the conventional practice described in the literature (37,39). We could detect conformational differences for ␤-sheet structures in the structure-sensitive amide I region (1610 -1700 cm Ϫ1 ) between the tested prion isoforms.…”

Section: Discussionmentioning

confidence: 99%

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Infrared Microspectroscopy Detects Protein Misfolding Cyclic Amplification (PMCA)-induced Conformational Alterations in Hamster Scrapie Progeny Seeds

Daus

Wagenführ

Thomzig

et al. 2013

Journal of Biological Chemistry

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Background: It is currently under discussion whether protein misfolding cyclic amplification (PMCA) alters strain properties of prions. Results: An improved infrared microspectroscopic approach combined with biochemical and bioassay data revealed altered strain properties of hamster 263K scrapie prions due to PMCA. Conclusion: PMCA can alter strain properties of 263K prions. Significance: Our analytical approach may help to improve the understanding of prion strain conversion.

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“…In order to avoid the use of antibodies as mentioned in previous paragraph, several spectroscopic methods, such as multispectral UV fluoroscopy, 54 fluorescence correlation spectroscopy, 55,56 magnetic resonance spectroscopy [57][58][59] or Fourier-transformed infrared spectroscopy (FTIR) 60,61 have been employed. The main disadvantages of these methods include requirements of expensive and sophisticated equipment as well as skilled operator.…”

Section: Scmentioning

confidence: 99%

Quantum dots and prion proteins

Šobrová

Blažková

Chomoucká

et al. 2013

Prion

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Analytical applications of Fourier transform-infrared (FT-IR) spectroscopy in microbiology and prion research

Abstract: International audienc

Cited by 245 publications

References 56 publications

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes

Infrared Microspectroscopy Detects Protein Misfolding Cyclic Amplification (PMCA)-induced Conformational Alterations in Hamster Scrapie Progeny Seeds

Quantum dots and prion proteins

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