Analytical and logistical improvements in doping-control analysis at the 2007 Pan-American Games

Doping control analysis at the Rio 2016 Olympic and Paralympic Games

“…Added 100 μL MSTFA/NH 4 I/2-mercaptoethanol (1000:2:6), incubated at 60°C for 20 minutes and analysed by GC-MS-MS. [3] hCG/LH Homogenised urine (400 μL) was loaded into the automated immunoassay analyser. [3,7] Anabolic agents, peptide hormones, growth factors, related substances and mimetics, β2 agonists, hormone and metabolic modulators, diuretics and masking agents, stimulants, narcotics, cannabinoids, glucocorticoids, β-blocker Two urine aliquots were used. Aliquot I: 10 μL of urine was diluted in 50 μL of 2% acetic acid, centrifuged (10 G/5 minutes) and stored (10°C).…”

“…The XXXI Summer Olympic Games (August [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]2016) and the XV Paralympic Games (September 7-18, 2016) were held in Rio de Janeiro, marking the first time that these events were organised in South America. Doping control analyses have been conducted in Brazil since 1989, were incorporated into the International Olympic Committee (IOC) accreditation in 2002, and were absorbed by the World Anti-Doping Agency (WADA) accreditation system in 2005.…”

Consequences of the formation of 3,4-dimethyl-5-phenyl-1,3-oxazolidine on the analysis of ephedrines in urine by gas chromatography and a new method for confirmation as N-trifluoroacetyl-O-t-butyldimethylsilyl ether derivatives

“…For characterization of the unknown peak and identification of its origin, the samples were treated following the method routinely used in our lab for stimulants by GC-NPD [31] adapted for ephedrine's quantification. Briefly, 5 mL of urine was spiked with 20 L of diphenylamine, used as internal standard at a concentration of 10 g/mL, followed by the addition of 0.2 mL of KOH (0.5 M), 2 mL of TBME and 1 g of Na 2 SO 4 .…”

Analysis of sibutramine metabolites as N-trifluoroacetamide and O-trimethylsilyl derivatives by gas chromatography–mass spectrometry in urine

“…The samples were analyzed by the method described by Solans et al [13] adapted in LAB DOP-LADETEC/IQ-UFRJ, for use in stimulants, narcotics and ␤-blockers Table 1 Diagnostic ions for sibutramine metabolites N-TMS-O-TMS (6) derivatives by GC-qMS detection and their respective relative intensities. screenings [14]. Following the addition of 83.6 mol of codeine to 5 mL of each urine sample, 1 mL of acetate buffer 1.1 M pH 5.2 (anhydrous sodium acetate/glacial acetic acid) and 50 L of ␤-glucuronidase Helix pomatia enzyme were added to each sample and incubated for 2 h at 55 • C. After cooling, the pH was adjusted to 9 with potassium hydroxide.…”