Analytical and clinical evaluation of DiaSorin Liaison® Calprotectin fecal assay adapted for serum samples

Macías-Muñoz, Laura; Frade‐Sosa, Beatriz; Iniciarte-Mundo, Jose; Hidalgo, Susana; Morlà, Rosa; Gallegos, Y.; Sanmarti, Raimón; Augé, Josep M.

doi:10.1002/jcla.24258

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…Calprotectin is a heterodimer formed by two proteins, S100A8 and S100A9, which are the most up-regulated proteins in rheumatoid arthritis [ 47 ]. Calprotectin can be measured in serum, plasma, and feces and exhibits potency as an inflammatory non-systemic biomarker [ 78 ]. Increased serum concentrations of calprotectin have been found in RA patients in contrast to healthy controls [ 78 ].…”

Section: Discussionmentioning

confidence: 99%

“…Calprotectin can be measured in serum, plasma, and feces and exhibits potency as an inflammatory non-systemic biomarker [ 78 ]. Increased serum concentrations of calprotectin have been found in RA patients in contrast to healthy controls [ 78 ]. A meta-analysis shows that blood calprotectin relates to disease risk, inflammation, and disease activity in RA patients [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Intestinal Permeability and Inflammation Bio-Markers in Patients with Rheumatoid Arthritis

et al. 2023

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Increased intestinal permeability and inflammation, both fueled by dysbiosis, appear to contribute to rheumatoid arthritis (RA) pathogenesis. This single-center pilot study aimed to investigate zonulin, a marker of intestinal permeability, and calprotectin, a marker of intestinal inflammation, measured in serum and fecal samples of RA patients using commercially available kits. We also analyzed plasma lipopolysaccharide (LPS) levels, a marker of intestinal permeability and inflammation. Furthermore, univariate, and multivariate regression analyses were carried out to determine whether or not there were associations of zonulin and calprotectin with LPS, BMI, gender, age, RA-specific parameters, fiber intake, and short-chain fatty acids in the gut. Serum zonulin levels were more likely to be abnormal with a longer disease duration and fecal zonulin levels were inversely associated with age. A strong association between fecal and serum calprotectin and between fecal calprotectin and LPS were found in males, but not in females, independent of other biomarkers, suggesting that fecal calprotectin may be a more specific biomarker than serum calprotectin is of intestinal inflammation in RA. Since this was a proof-of-principle study without a healthy control group, further research is needed to validate fecal and serum zonulin as valid biomarkers of RA in comparison with other promising biomarkers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of Intestinal Permeability and Inflammation Bio-Markers in Patients with Rheumatoid Arthritis

et al. 2023

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“…This analyzer was designed for the quantification of fecal calprotectin so results are expressed in µg/g. Based on previous publication [ 9 ] a conversion factor of 2.5 was used to transform these units into ng/mL for the pleural fluid sample. The calibration curve was also transformed, being the measurement range of 12.50–2,000 ng/mL.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of DiaSorin Liaison^® calprotectin fecal assay adapted for pleural effusion

de Paz Poves,

Barneo-Caragol,

Cillero Sánchez

et al. 2023

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Objectives Calprotectin (CP) is a calcium and zinc binding protein that is widely measured on faecal samples but its determination in other biological fluids might be of interest. The aim of this work was to validate the measurement of CP in pleural fluid by chemiluminescence. Methods LIAISON®XL, a fully automated chemiluminescence analyzer, was used for CP quantification on pleural fluid. A validation protocol was designed using both quality control materials provided by the manufacturer and pools of pleural fluid samples. Stability, imprecision, bias, linearity, detection capability and carry over effect were evaluated. Results CP was stable on pleural fluid at least one week, under refrigerated conditions, and four weeks at −80 °C. The observed intra- and inter-day imprecision was between 2.2 and 6.49 %, with a negative bias under 5.51 %. The linearity of the method was verified up to 2,000 ng/mL. The LoQ for the assay was 48.52 ng/mL. A statistically significant carry-over effect was observed after measuring CP concentrations above the upper limit of linearity, but given the observed magnitude, a clinically relevant impact should not be expected. Conclusions Diasorin Liaison® calprotectin assay allows reliable measurement of CP in pleural fluid.

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“…Este analizador fue originariamente diseñado para la cuantificación de calprotectina fecal, por lo que presenta los resultados en µg/g. Basándonos en un estudio previo [ 9 ], utilizamos un factor de conversión de 2,5 para transformar dichas unidades a ng/ml, para las muestras de líquido pleural. También se transformó la curva de calibración, siendo el rango de medida de 12,50–2000 ng/mL.…”

Section: Materiales Y Métodosunclassified

Evaluación de la prueba fecal Liaison^® Calprotectin de DiaSorin adaptada al derrame pleural

de Paz Poves,

Barneo-Caragol,

Cillero Sánchez

et al. 2023

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Resumen Objetivos La calprotectina (CP) es una proteína de unión a calcio y zinc que se suele determinar en muestras fecales, aunque su cuantificación en otros fluidos biológicos podría ser de interés. El objetivo del presente estudio es validar la determinación de CP en líquido pleural mediante quimioluminiscencia. Métodos Para la cuantificación de CP en líquido pleural, se utilizó LIAISON®XL, un autoanalizador de quimioluminiscencia. Se diseñó un protocolo de validación empleando tanto materiales de control de calidad suministrados por el fabricante, como soluciones de muestras de líquido pleural. Se evaluaron la estabilidad, imprecisión, sesgo, linealidad, capacidad de detección y efecto de arrastre. Resultados La CP permaneció estable en líquido pleural refrigerado durante al menos una semana, y durante cuatro semanas a −80 °C. La imprecisión intradía e interdía observada fue del 2,2 % y del 6,49 %, respectivamente, con un sesgo negativo del 5,51 %. La linealidad del método se verificó hasta los 2000 ng/mL. El límite de cuantificación (LoQ) de la prueba fue de 48,52 ng/mL. Se observó un efecto de arrastre estadísticamente significativo tras medir concentraciones de CP superiores al límite máximo de linealidad. Sin embargo, dada la magnitud observada, no se debe esperar un impacto clínicamente relevante. Conclusiones La prueba Liaison® Calprotectin de DiaSorin es fiable para la determinación de CP en líquido pleural.

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Analytical and clinical evaluation of DiaSorin Liaison® Calprotectin fecal assay adapted for serum samples

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 6 publications

References 28 publications

Assessment of Intestinal Permeability and Inflammation Bio-Markers in Patients with Rheumatoid Arthritis

Assessment of Intestinal Permeability and Inflammation Bio-Markers in Patients with Rheumatoid Arthritis

Evaluation of DiaSorin Liaison^® calprotectin fecal assay adapted for pleural effusion

Evaluación de la prueba fecal Liaison^® Calprotectin de DiaSorin adaptada al derrame pleural

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Analytical and clinical evaluation of DiaSorin Liaison® Calprotectin fecal assay adapted for serum samples

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 6 publications

References 28 publications

Assessment of Intestinal Permeability and Inflammation Bio-Markers in Patients with Rheumatoid Arthritis

Assessment of Intestinal Permeability and Inflammation Bio-Markers in Patients with Rheumatoid Arthritis

Evaluation of DiaSorin Liaison® calprotectin fecal assay adapted for pleural effusion

Evaluación de la prueba fecal Liaison® Calprotectin de DiaSorin adaptada al derrame pleural

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Product

Resources

About

Evaluation of DiaSorin Liaison^® calprotectin fecal assay adapted for pleural effusion

Evaluación de la prueba fecal Liaison^® Calprotectin de DiaSorin adaptada al derrame pleural