Analysis on migration and activation of live macrophages on transparent flat and nanostructured titanium

Lee, So-Young; Choi, Jungil; Shin, Sangwoo; Im, Yeon-Min; Song, Ji Yeon; Kang, Sang Soo; Nam, Tae-Hyun; Webster, Thomas J.; Kim, Sang-Hyun; Khang, Dongwoo

doi:10.1016/j.actbio.2011.01.006

Cited by 100 publications

(78 citation statements)

References 33 publications

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“…Thalji et al [70] reported that pro-inflammatory genes were downregulated in cells adhering to nano-roughened surfaces when compared to those on micro-roughened surfaces implanted into rats. This indicates improved osseointegration potential and supports the finding of Lee et al [48] that macrophages are not activated by these surfaces. Mengatto et al [71] demonstrated in vivo that osseointegration may be dependent on vitamin D availability, involves genes that produce proteins of the ECM, and includes circadian rhythm gene regulation.…”

Section: Microarrayssupporting

confidence: 80%

“…Optical microscopy is limited to techniques that utilize reflected light due to the opaque nature of titanium. However, by coating glass coverslips with a 35 nm thin film of titanium, Lee et al [48] were able to use incident light microscopy to track living macrophages. These immune cells exhibited reduced migration on the nanoroughened surface, indicating a reduced immune response.…”

Section: Cell Morphologymentioning

confidence: 99%

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Understanding the biological responses of nanostructured metals and surfaces

Lowe

Reiss

2014

IOP Conf. Ser.: Mater. Sci. Eng.

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Section: Microarrayssupporting

confidence: 80%

Section: Cell Morphologymentioning

confidence: 99%

Understanding the biological responses of nanostructured metals and surfaces

Lowe

Reiss

2014

IOP Conf. Ser.: Mater. Sci. Eng.

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“…Gold ions released from the surfaces could result in an anti-inflammatory reaction, 54 but measurements of gold in the media (data not shown) indicated low yet equal levels in smooth and nanostructured gold samples, whereas elevated levels of IL-10 were only seen for nanostructured gold samples, indicating that the effect is caused by the nanofeatures, rather than gold ions leaking from the surface. Other studies have found a lower gene expression of IL-1β and TNF-α on nanomodified TiO surfaces (rms 4.8) compared with smooth surfaces, using a murine macrophage cell line, 55 and less TNF-α protein as well as more rounded human monocytes on 20 nm pores compared with 200 nm pores, 11 indicating that small surface nanofeatures may have a down-regulatory effect on monocytes/macrophages.…”

Section: Monocyte Activation In Response To Different Substrates and mentioning

confidence: 99%

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Svensson¹,

Forsberg²,

Hulander³

et al. 2014

IJN

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The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis .

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“…Again, implementing nanoscale roughness on the granules was important because numerous studies have indicated that nanoscale roughness can decrease inflammation and immune system recognition allowing the granules to reach their destination and release drugs. 32,33 Of course, more testing is necessary to confirm this. Moreover, the drug content of the guar gum and Eudragit FS30D coated granules for 5-FU was found to be 96.5%.…”

Section: Drug Content In 5-fu Granules and Characterizationmentioning

confidence: 99%

“…Moreover, an important part of this work was to create micron granules with nanostructured roughness, since numerous studies have demonstrated that nanoroughness can decrease inflammation and immune system recognition. 32,33 This is because nanoroughness can alter the surface energy of granules and control initial protein adsorption (such as decreased IgG adsorption) to inhibit inflammatory cell responses. Such events would allow the drug capsule to be more effective at delivering drugs to targeted sites.…”

Section: Introductionmentioning

confidence: 99%

A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media

Kotla¹,

Singh²,

Maddiboyina³

et al. 2016

IJN

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The aim of this study was to develop a novel microbially triggered and animal-sparing dissolution method for testing of nanorough polysaccharide-based micron granules for colonic drug delivery. In this method, probiotic cultures of bacteria present in the colonic region were prepared and added to the dissolution media and compared with the performance of conventional dissolution methodologies (such as media with rat cecal and human fecal media). In this study, the predominant species (such as Bacteroides, Bifidobacterium, Lactobacillus species, Eubacterium and Streptococcus ) were cultured in 12% w/v skimmed milk powder and 5% w/v grade “A” honey. Approximately 10 10 –10 11 colony forming units m/L of probiotic culture was added to the dissolution media to test the drug release of polysaccharide-based formulations. A USP dissolution apparatus I/II using a gradient pH dissolution method was used to evaluate drug release from formulations meant for colonic drug delivery. Drug release of guar gum/Eudragit FS30D coated 5-fluorouracil granules was assessed under gastric and small intestine conditions within a simulated colonic environment involving fermentation testing with the probiotic culture. The results with the probiotic system were comparable to those obtained from the rat cecal and human fecal-based fermentation model, thereby suggesting that a probiotic dissolution method can be successfully applied for drug release testing of any polysaccharide-based oral formulation meant for colonic delivery. As such, this study significantly adds to the nanostructured biomaterials’ community by elucidating an easier assay for colonic drug delivery.

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Analysis on migration and activation of live macrophages on transparent flat and nanostructured titanium

Cited by 100 publications

References 33 publications

Understanding the biological responses of nanostructured metals and surfaces

Understanding the biological responses of nanostructured metals and surfaces

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media

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