Analysis on heat stress-induced hyperphosphorylation of stathmin at serine 37 in Jurkat cells by means of two-dimensional gel electrophoresis and tandem mass spectrometry

c-Jun N-terminal Kinase Phosphorylation of Stathmin Confers Protection against Cellular Stress

“…During growth factor stimulation, both STMN Ser-25 and Ser-38 have been previously established as target sites for prolinedirected kinases such as the cyclindependent kinases and members of the MAPK family that can, thus, regulate STMN activity in neuron of the brain and proliferating cancer cells (36 -40). In contrast, the phosphorylation of STMN in response to stress stimuli and pro-inflammatory cytokines has been noted (22,25,41,42), but the regulation of these events and specifically the protein kinases involved have been less intensively studied.…”

Endoplasmic reticulium protein profiling of heat-stressed Jurkat cells by one dimensional electrophoresis and liquid chromatography tandem mass spectrometry

“…In previous studies we performed nuclear protein profiling of Jurkat cells during heat stress generating some interesting data (Yuan et al 2007;Nakamura et al 2006) leading directly to the question on the effects of heat stress on ER. Thus we continued these studies using proteomic analysis of heat stressed Jurkat cells.…”

“…However, very few detailed proteomic studies have been carried out on ER isolated from human Jurkat T leukemia cells. We previously reported that a cytosolic phosphoprotein of stathmin, which mediates extracellular signals in Jurkat cells, is phosphorylated by cdk1 during heat stress (Nakamura et al 2006), and we reported the nuclear protein profiling of Jurkat cells by heat stress (Yuan et al 2007).…”

“…To elucidate the underlying mechanisms by which heat stress induces apoptosis (Nakamura et al 2006), we performed a comprehensive proteomic analysis to identify molecules mediating the process. After matching with 1-DE maps, 14 protein bands were selected for further investigation by LC-MS/MS.…”

Differential Protein Expression in EC304 Gastric Cancer Cells Induced by Alphastatin

“…Alphastatin, as a new angiogenesis inhibitor, strong inhibits angiogenesis, and the exploration of its mechanism is helpful for the correct application of this compound. The study of alphastatin in vascular endothelial cell angiogenesis and its mechanisms of action would be helpful for not only understanding alphastatin biological activity, but might also provide an alternative in the clinical treatment of gastric cancer (Nakamura et al, 2006). Our previous experiments explored the effects of alphastatin on the biological characteristics of human vascular endothelial cells.…”