Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis

Huang, Fuqiang; Dang, Zhisheng; Suzuki, Yutaka; Horiuchi, T; Yagi, Kinpei; Kouguchi, Hirokazu; Irie, Takao; Kim, Kyeong-Soon; Oku, Yuzaburo

doi:10.1371/journal.pntd.0004634

Cited by 33 publications

(40 citation statements)

References 61 publications

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Section: Introductionmentioning

confidence: 99%

“…In China, AE is also known as "worm cancer", a fatal and serious disease [5] which is difficult to treat with poor prognosis [6]. After AE infection, the fatality rate of untreated can be large than 90%, even as high as 94% [6,7]. Echinococcosis not only affects human health and causes human lives to be lost due to significant morbidity and mortality, but also brings about economic loss related to livestock husbandry [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Knowledge, attitudes and practices regarding echinococcosis in Xizang Autonomous Region, China

Nima

et al. 2020

BMC Public Health

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Background: Echinococcosis is a neglected zoonotic parasitic disease caused echinococcus parasitizes, poseing a significant economic burden on both humans and animals. There are limited studies on echinococcosis in China, especially Xizang Autonomous Region, although the area is endemic area for echinococcosis. The study aimed to provide information for strategic prevention against this disease. Methods: A cross-sectional survey was conducted among the residents in Xizang Autonomous Region, China to evaluate their knowledge, attitudes and practices on endemicity of echinococcosis. A face-to-face survey was conducted to collect the data using a well-designed questionnaire. The contents included basic personal information, knowledge, attitudes and practices about echinococcosis, personal hygiene and behavior habits, dog feeding and whether they had received the information on echinococcosis, and so on. We surveyed 840 persons in practice. All data analysis was performed using Epi Info 7.2. Results: Of the total particpants, 86.8% had a primary education level or below (including primary and illiterate), and even 45.0% were illiterate. Farmers and herdsmen represent the main occupations in this study. People who know all the echinococcosis-related knowledge in the questionnaire only accounted for 8.7% of the participants. However, none of the participants was aware of routes of echinococcosis infection in human or dogs. The data showed participants with higher educational background had the high awareness rate of echinococcosis-related knowledge or attitudes (chi-square for trend, χ 2 = 21.23, p<0.05 & χ 2 = 48.43, p<0.05). In addition, The percentage of the participant with awareness of echinococcosis-related practices was associated with their age and principle occupation (χ 2 = 52.72, p<0.05 & χ 2 = 20.63, p<0.05). Conclusions: Xizang Autonomous Region is an epidemic area of Echinococcosis. The prevalence of the disease has been largely due to the lack of knowledge, awareness, and poor hygiene practice in local residences. Therefore, effective disease prevention education and awareness campaigns in community will be significantly helpful in prevention and control of echinococcosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Knowledge, attitudes and practices regarding echinococcosis in Xizang Autonomous Region, China

Nima

et al. 2020

BMC Public Health

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“…In order to determine the infectivity and viability of Echinococcus species eggs and metacestodes by quantification of mRNA in fecal and hydatid cyst samples, further studies on expression patterns of genes in these different developmental stages of the parasites would be necessary. An example for this is a study on the expression profiles of oncospheres and early stage metacestodes of E. multilocularis (Huang et al 2016). However, regarding E. felidis, RNA sequencing has not been conducted.…”

Section: Discussionmentioning

confidence: 99%

Development of real-time polymerase chain reaction assays allowing molecular detection ofEchinococcus felidis, Echinococcus granulosussensu stricto, andEchinococcus canadensisin carnivore feces samples, animal and human hydatid cyst material from Uganda and Kenya

Kopp

2017

Preprint

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___________________________________________________________________________First evaluations on field samples, including carnivore feces, animal and human hydatid cyst material from Uganda and Kenya, showed specific amplification of two target regions of the mitochondrial genome of Echinococcus species according to melt and high-resolution melt curve analyses of the developed real-time PCR assays. Consecutive sequencing of PCR products revealed that, apart from Echinococcus felidis, sequences of two other tapeworm species, Echinococcus granulosus sensu stricto and Echinococcus canadensis, which are also endemic in East Africa, were detected by the developed real-time PCR assays. ___________________________________________________________________________ IntroductionThe aim of the present study was to develop a rapid, sensitive, and safe method for molecular detection of Echinococcus felidis, and possibly, related tapeworm species. We first investigated nucleic acid extraction methods suitable for isolating templates directly from fecal samples of carnivores and hydatid cyst material of intermediate hosts in the field without tedious laboratory based enrichment methods, such as digestion or flotation methods. Then, we developed real-time PCR assays which target two different mitochondrial genome regions of E. felidis.

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“…Human alveolar echinococcosis (AE), is caused by the larval stage of the tapeworm Echinococcus multiloculari s, one of the most lethal of the human helminthiases. Untreated AE has a fatality rate of >90% in humans, causing great public health concern in the northern hemisphere [ 1 , 2 ]. China is believed to account for the majority of global AE cases, where 230,000 individuals presently suffer from the disease with a total population at risk of some 22.6 million in 7 provinces, representing nearly 50% of China’s total area [ 1 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development

Chen

Yan

et al. 2018

PLoS Negl Trop Dis

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BackgroundHepatocyte-based metacestode culture is an attractive method to study alveolar echinococcosis (AE), but it is limited by the relatively short lifespan of cultured hepatocytes in maintaining their normal function.Methodology/principal findingsWe describe a three-dimensional (3D) hepatic culture system developed from co-cultured hepatocytes and mesenchymal stem cells using a collagen scaffold to study the development of Echinococcus multilocularis larvae. This 3D culture system preserved the function of hepatocytes for a longer period of time than their monolayer counterparts, with albumin secretion, 7-ethoxyresorufin O-deethylation activity, urea synthesis, CYP3A4 and CYP2D6 activity being highly preserved for 21–28 days. The expression levels of hepatocyte-specific genes including CLDN-3, Bsep, AFP, G6P, A1AT, CYP3A4 and NR1I3 were significantly higher in the 3D cultured system compared with their monolayer counterparts after 14-days in culture. Additionally, in the presence of 3D cultured hepatocytes, 81.2% of E. multilocularis protoscoleces rapidly de-differentiated into infective vesicles within eight weeks. Transcriptomic analyses revealed 807 differentially expressed genes between cultured vesicles and protoscoleces, including 119 genes uniquely expressed in protoscoleces, and 242 genes uniquely expressed in vesicles. These differentially expressed genes were mainly involved in parasite growth relating to the G-protein coupled receptor activity pathway, substrate-specific transmembrane transporter activity, cell-cell adhesion process, and potentially with neuroactive ligand-receptor interaction.Conclusions/significanceThis culture system provides a valuable advance in prolonging hepatocyte functionality, a foundation for future in-depth analysis of the host-parasite interaction in AE, and a useful model to evaluate potential therapeutic strategies to treat AE.

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Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis

Cited by 33 publications

References 61 publications

Knowledge, attitudes and practices regarding echinococcosis in Xizang Autonomous Region, China

Knowledge, attitudes and practices regarding echinococcosis in Xizang Autonomous Region, China

Development of real-time polymerase chain reaction assays allowing molecular detection ofEchinococcus felidis, Echinococcus granulosussensu stricto, andEchinococcus canadensisin carnivore feces samples, animal and human hydatid cyst material from Uganda and Kenya

Three-dimensional hepatocyte culture system for the study of Echinococcus multilocularis larval development

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