Analysis on Donor and Isolation-Related Factors of Successful Isolation of Human Islet of Langerhans From Human Cadaveric Donors

A Multicenter Study: North American Islet Donor Score in Donor Pancreas Selection for Human Islet Isolation for Transplantation

“…To standardize the pancreatic donors using a combined donor variables approach, O’Gorman et al published the first study with an islet donor score resulting from variables of 326 donors between 1999 and 2004 in a single center (26). As islet mass required for transplant remains >5,000 IEQ per kg of recipient BW, in our analysis we defined successful islet isolation as those with a post-purification islet yield greater than 400,000 IEQ, which is the highest cut off value among those defined in the previous studies (9,11,12,22). With this rigorous definition, transplant can be accomplished in most of the patients, all those with weight ≤80 kg.…”

Islet vs. pancreas transplantation in Brazil: Defining criteria for pancreas allocation decision

“…17 Some other variables, such as the donor age, cold ischemia time and gender were separately identified as predictors of good isolation yield in the few studies available on the subject. [14][15][16][17][18][19][20] The present study was able to identify some variables that influence islet yield. In the univariate analysis BMI, digestion time and the presence of fatty infiltrates during harvesting were found to be associated with a better islet isolation outcome.…”

“…However, several other variables have been reported, such as donors' age, use of vasoactive drugs, cold ischemia time, pancreas weight and digestion time. [14][15][16][17][18][19][20] In 2005, the USA modified the criteria for allocation of pancreata obtained from deceased donors, with those from donors (cold ischemia time, use of dopamine and creatinine level) and included in the multivariate analysis, which identified only the donor's BMI to be a predictor of pancreatic islet isolation success in a Brazilian donor sample. The other variables mentioned in the studies cited above were also analyzed, but were not found to be statistically significant in predicting the islet isolation outcome, probably due to a smaller number of donors included in the present work.…”

“…Lakey et al 15 and Kin et al 18 defined as a good outcome for islet isolation yields of 100,000 IEQs, while Hanley et al adopted yields of 250,000 IEQs as satisfactory. Matsumoto et al 16 established the value of 300,000 IEQs as a reference for a good islet isolation outcome.…”

Pancreatic Islet Cell Transplantation: Update and New Developments

“…10 In general, islet cell procurement results in islet transplantation 18% (or less) to 67% of the time in the best series reported so far. [11][12][13] Traditionally, the procured pancreas is preserved in University of Wisconsin (UW) solution, which is the usual preservation solution for abdominal organ procurement. Currently, the majority of centers use a two-layer method (TLM), 14 -17 where the upper fluid layer is UW solution and the lower layer is oxygenated perfluorocarbon.…”