Analysis of γ-Aminobutyric Acid Content in Fermented Plant Products by HPLC/UV

Abstract

γ-Aminobutyric acid (GABA) content in fermented plant products and their main plant materials (aerial part of Acanthopanax sessiliflorus, fruit of Crataegus pinnatifida, and whole plant of Morus alba) was determined by high-performance liquid chromatography. GABA was quantified using a reversephase column with a gradient elution program (water:acetonitrile =90:10 to 0:100 for 40 min). UV detection was conducted at 280 nm. GABA content was measured in fermented plant products (15.07 mg/g), aerial part of A. sessiliflorus (4.49 mg/g), fruit of C. pinnatifida (10.59 mg/g), and whole plant of M. alba (2.31 mg/g). The presence of GABA in fermented plant products, including A. sessiliflorus, C. pinnatifida, and M. alba is important in industrial application for health supplements.

Dietary γ-Aminobutyric Acid (GABA) Promotes Growth and Resistance to Vibrio alginolyticus in Whiteleg Shrimp Litopenaeus vannamei

“…Ingredients such as fishmeal have some endogenous GABA content, and therefore, diets CON and OTC show some endogenous GABA composition. The composition of the GABA content in each experimental diet was evaluated and analyzed using high-performance liquid chromatography (HPLC, Sykam, Eresing, Germany) following the procedure of Lee et al [38]. 20 g of each diet was ground and extracted with 100% of MeOH (3 × 100 mL) for sample preparation.…”

Phytochemistry and Pharmacology of Eleutherococcus sessiliflorus (Rupr. & Maxim.) S.Y.Hu: A Review

“…However, research on the amino acids present in E. sessiliflorus has been limited. Only five amino acids have been identified in this plant, namely ethanolamine ( 263 ), 3-hydroxy-L-proline ( 267 ), γ -aminobutyric acid ( 268 ), phenylalanine ( 270 ), and L-norvaline ( 271 ) [ 14 , 20 , 67 , 75 ]. L-Norvaline (250 mg/L in animals’ water) reverses cognitive decline and synaptic loss in a murine model of Alzheimer’s disease [ 76 ].…”