Analysis of Zearalenone in Cereal and Swine Feed Samples Using an Automated Flow-Through Immunosensor

Urraca, Javier L.; Benito‐Peña, Elena; Pérez-Conde, C.; Moreno‐Bondi, María C.; Pestka, James J.

doi:10.1021/jf048092p

Cited by 68 publications

(31 citation statements)

References 30 publications

(46 reference statements)

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“…Flow-through immunoassays have been developed for screening OTA in green and roasted coffee [114,115], AFB1 in nuts [116], and ZEA in cereals and feed samples [117]. Although many different rapid strip tests have been developed for detection of major mycotoxins in different food commodities, they are not commonly used in the field and not commercially successful due to problems related to sensitivity, cost, and accuracy [109].…”

Section: Analysis Of Mycotoxins In Foodmentioning

confidence: 99%

Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food

Alshannaq

2017

IJERPH

877

573

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Mycotoxins are toxic secondary metabolites produced by certain filamentous fungi (molds). These low molecular weight compounds (usually less than 1000 Daltons) are naturally occurring and practically unavoidable. They can enter our food chain either directly from plant-based food components contaminated with mycotoxins or by indirect contamination from the growth of toxigenic fungi on food. Mycotoxins can accumulate in maturing corn, cereals, soybeans, sorghum, peanuts, and other food and feed crops in the field and in grain during transportation. Consumption of mycotoxin-contaminated food or feed can cause acute or chronic toxicity in human and animals. In addition to concerns over adverse effects from direct consumption of mycotoxin-contaminated foods and feeds, there is also public health concern over the potential ingestion of animal-derived food products, such as meat, milk, or eggs, containing residues or metabolites of mycotoxins. Members of three fungal genera, Aspergillus, Fusarium, and Penicillium, are the major mycotoxin producers. While over 300 mycotoxins have been identified, six (aflatoxins, trichothecenes, zearalenone, fumonisins, ochratoxins, and patulin) are regularly found in food, posing unpredictable and ongoing food safety problems worldwide. This review summarizes the toxicity of the six mycotoxins, foods commonly contaminated by one or more of them, and the current methods for detection and analysis of these mycotoxins.

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Section: Analysis Of Mycotoxins In Foodmentioning

confidence: 99%

Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food

Alshannaq

2017

IJERPH

877

573

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“…Array biosensors have been developed using competitive-based immunoassays for the simultaneous detection of multiple mycotoxins, including ochratoxin A, fumonisin B, aflatoxin B1, and deoxynivalenol, on a single waveguide surface by imaging the fluorescent pattern onto a CCD (charge-coupled device) camera (Sapsford et al 2006). Other formats with fluorescence detection include automated flow-through immunosensors with enzyme-labelled mycotoxin derivatives (Urraca et al 2005). Electrochemical immunosensors with surface-adsorbed antibodies on screen-printed carbon electrodes have been fabricated for the detection of aflatoxin M1 in milk (Micheli et al 2005) and, in an array configuration, for the detection of aflatoxin B1 (Pemberton et al 2006).…”

Section: Immunochemical Techniquesmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

310

215

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Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.

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“…The natural occurrence of ZON has been reported in Germany, Brazil, Bulgarian, Mexico, Korea, etc. (Sohn et al 1999;Silva et al 2001;Schollenberger et al 2002;Urraca et al 2005;Briones-Reyes et al 2007;Manova et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Determination of zearalenone in traditional Chinese medicinal plants and related products by HPLC–FLD

Zhang

Liu

Logrieco

et al. 2011

Food Additives & Contaminants: Part A

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A HPLC-FLD method has been developed and validated for zearalenone (ZON) in 107 widely consumed Chinese medicinal herbs and related products collected from different regions of China. Samples were extracted with methanol/water (80 : 20, v/v), and the extracts were cleaned-up through immunoaffinity columns (IAC). ZON was quantified by HPLC with fluorescence detection. Recoveries from three different medicinal herbs spiked with ZON at levels ranging from 30 to 600 µg kg(-1) were from 80.8 to 98.3%. The limit of detection was 9.5 µg kg(-1), based on a signal-to-noise ratio of 3 : 1. Naturally occurring ZON was only found in coix seed medicinal herb (all nine samples), with levels ranging from 18.7 to 211.4 µg kg(-1). Positive results were confirmed by UV spectrum and LC-ESI-MS/MS. This is the first report of ZON contamination of a Chinese medicinal herb.

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Analysis of Zearalenone in Cereal and Swine Feed Samples Using an Automated Flow-Through Immunosensor

Cited by 68 publications

References 30 publications

Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food

Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food

Mycotoxin analysis: An update

Determination of zearalenone in traditional Chinese medicinal plants and related products by HPLC–FLD

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