Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock

Worland, Alyssa M; Czajka, Jeffrey J.; Xing, Yun; Harper, Willie F.; Moore, Aryiana; Xiao, Zhengyang; Han, Zhenlin; Wang, Yechun; Su, Wei; Tang, Yinjie J.

doi:10.1016/j.mec.2020.e00130

Cited by 17 publications

(12 citation statements)

References 53 publications

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“…To enable two different heterotroph stains to grow and express engineered pathways in a co-culture, the chemical environment of the medium in system must be carefully chosen. As the base medium for cyanobacterial setup and co-culture, BG11 medium is not formulated for optimal growth of either heterotrophic partner (e.g., BG11 contains nitrate as a nitrogen source, whereas the medium for either heterotroph conventionally supplies ammonia 35,36 ). Additionally, the reactive oxygen species (ROS) generated as byproducts from photosynthesis is another stressor to P. putida and Y. lipolytica.…”

Section: Resultsmentioning

confidence: 99%

“…1, 2). This observation was expected because the engineered Y. lipolytica strain requires a minimum threshold of exogenously supplied amino acids for β-carotene production 36 . Because cyanobacterial culture contained only low levels of free extracellular amino acids, the residual rich nutrients in the medium could quickly become depleted would limit Y. lipolytica β-carotene production.…”

Section: Development Of a Continuous Co-culture Platform (With Sucros...mentioning

confidence: 95%

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Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels

Zhao

Sengupta

Tan

et al. 2022

Sci Rep

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Engineered cyanobacterium Synechococcus elongatus can use light and CO2 to produce sucrose, making it a promising candidate for use in co-cultures with heterotrophic workhorses. However, this process is challenged by the mutual stresses generated from the multispecies microbial culture. Here we demonstrate an ecosystem where S. elongatus is freely grown in a photo-bioreactor (PBR) containing an engineered heterotrophic workhorse (either β-carotene-producing Yarrowia lipolytica or indigoidine-producing Pseudomonas putida) encapsulated in calcium-alginate hydrogel beads. The encapsulation prevents growth interference, allowing the cyanobacterial culture to produce high sucrose concentrations enabling the production of indigoidine and β-carotene in the heterotroph. Our experimental PBRs yielded an indigoidine titer of 7.5 g/L hydrogel and a β-carotene titer of 1.3 g/L hydrogel, amounts 15–22-fold higher than in a comparable co-culture without encapsulation. Moreover, 13C-metabolite analysis and protein overexpression tests indicated that the hydrogel beads provided a favorable microenvironment where the cell metabolism inside the hydrogel was comparable to that in a free culture. Finally, the heterotroph-containing hydrogels were easily harvested and dissolved by EDTA for product recovery, while the cyanobacterial culture itself could be reused for the next batch of immobilized heterotrophs. This co-cultivation and hydrogel encapsulation system is a successful demonstration of bioprocess optimization under photobioreactor conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Development Of a Continuous Co-culture Platform (With Sucros...mentioning

confidence: 95%

Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels

Zhao

Sengupta

Tan

et al. 2022

Sci Rep

Self Cite

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“…Metabolic engineering of Y. lipolytica to produce bioactive compounds seems to be a good choice, especially when the yeast is employed to utilize common forestry and agricultural wastes such as cellulose, inulin, starch, or xylan, unused by wt strains [ 77 , 190 ], and/or to produce novel compounds that are not produced by the wt yeast. Engineered Y. lipolytica strains produce several heterologous valuable metabolites such as carotenoids [ 191 , 192 ]; terpenes, e.g., limonene [ 193 ]; polyketides with various biological activities; and aromatic amino-acid-derived molecules [ 194 , 195 ].…”

Section: Added-value Compounds For Industrial Applicationmentioning

confidence: 99%

Yarrowia lipolytica as an Alternative and Valuable Source of Nutritional and Bioactive Compounds for Humans

Jach

Malm

2022

Molecules

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Yarrowia lipolytica, an oleagineous species of yeast, is a carrier of various important nutrients. The biomass of this yeast is an extensive source of protein, exogenous amino acids, bioavailable essenctial trace minerals, and lipid compounds as mainly unsaturated fatty acids. The biomass also contains B vitamins, including vitamin B12, and many other bioactive components. Therefore, Y. lipolytica biomass can be used in food supplements for humans as safe and nutritional additives for maintaining the homeostasis of the organism, including for vegans and vegetarians, athletes, people after recovery, and people at risk of B vitamin deficiencies.

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“…Section 4.2.4). Nevertheless, any cell factory design relies at first on a precise understanding of cellular metabolism that can only be built through genomic, transcriptomic, metabolomic or fluxomic analyses [295][296][297][298]. Transcriptomics tools have notably been applied, at the Poznan University of Life Sciences (Poznan, Poland), to studying the consequences of an overproduction of secreted heterologous proteins, which can be a metabolic burden and a source of stress for yeast cells [299].…”

Section: Genome-scale Omics Tools and Metabolic Modelsmentioning

confidence: 99%

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

Madzak

2021

JoF

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Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast Yarrowia lipolytica appears as one of the most attractive for a large range of white biotechnology applications, from heterologous proteins secretion to cell factories process development. The past, present and potential applications of wild-type, traditionally improved or genetically modified Yarrowia lipolytica strains will be resumed, together with the wide array of molecular tools now available to genetically engineer and metabolically remodel this yeast. The present review will also provide a detailed description of Yarrowia lipolytica strains and highlight the natural biodiversity of this yeast, a subject little touched upon in most previous reviews. This work intends to fill this gap by retracing the genealogy of the main Yarrowia lipolytica strains of industrial interest, by illustrating the search for new genetic backgrounds and by providing data about the main publicly available strains in yeast collections worldwide. At last, it will focus on exemplifying how advances in engineering tools can leverage a better biotechnological exploitation of the natural biodiversity of Yarrowia lipolytica and of other yeasts from the Yarrowia clade.

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Analysis of Yarrowia lipolytica growth, catabolism, and terpenoid biosynthesis during utilization of lipid-derived feedstock

Cited by 17 publications

References 53 publications

Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels

Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels

Yarrowia lipolytica as an Alternative and Valuable Source of Nutritional and Bioactive Compounds for Humans

Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

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