Analysis of Yak MUC1 Protein Polymorphisms and the Corresponding VNTR Structure

Zheng, Yusheng; Fan, Qipeng; Liu, Yufang; Zhao, Xingbo; He, Xiaping; Jin, Suyu

doi:10.1080/10495390903209928

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…In humans, the number of repeats at the VNTR region ranges from 25 and 125 with size ranging from 1,000-5,000 bp (Ando et al 1998). In ruminants, the VNTR region varied from 1,500-3,000 bp in goats (Sacchi et al 2004), from 778 to 1894bp in bovines (Rasero et al 2002;Souza et al 2007;Sando et al 2009), four alleles of approximately 1500 bp in sheep (Rasero et al 2007) and from 1206 to 1566 bp in yaks (Zheng et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Associations between MUC1 gene polymorphism and resistance to mastitis, milk production and fertility traits in Murrah water buffaloes

Rosa

Moreira

Barbero

et al. 2020

Journal of Applied Animal Research

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MUC1 is a glycoprotein mucin expressed in apical cells of luminal tissues, such as mammary gland luminal cells, which the main function is protect the cell surface from the environment microorganisms. The aim of this study were to identify the polymorphism of MUC1 gene in Murrah breed and to analyze the association between molecular marker and somatic cell counting, milk yield, fat yield, fat percentage, protein yield, protein percentage, age at first calving and calving interval. A total of 179 primiparous Murrah buffaloes were genotyped by PCR technique. Milk-recording were performed periodically to determine fat and protein content and somatic cell count. Three alleles of different lengths were amplified and named allele 1, 2 and 3, respectively. The allele 2 was more frequent followed by allele 3. The MUC1 gene was highly associated with traits related to mastitis resistance and milk percentage. Also was demonstrated a mild association between MUC1 gene and milk protein synthesis and a moderate effect on reproductive trait. The MUC1 gene is polymorphic in water buffaloes of Murrah breed and might be a potential marker to marker-assisted selection for intramammary infection and some milk production traits.

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Section: Discussionmentioning

confidence: 99%

Associations between MUC1 gene polymorphism and resistance to mastitis, milk production and fertility traits in Murrah water buffaloes

Rosa

Moreira

Barbero

et al. 2020

Journal of Applied Animal Research

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“…In our study, we examined four SNPs, published earlier by Madsen and coworkers 42 , in 29 Polish Landrace × Duroc, Pietrain crossbreed pigs. We found that in the Polish Landrace crossbreed with Duroc × Pietrain, for SNP 594 C > G, 86% of pigs had the SNP (CG or GG) predisposing to increased amount of lumbar back fat, total percentage of lipids, and intramuscular fatness 4 . This may explain why we were successful in obtaining a high level of fatness and observed the development of insulin resistance in the group of pigs fed the HE diet.…”

Section: Discussionmentioning

confidence: 82%

“…In recent years, a few studies have been published on porcine SNPs in the FTO gene. Interestingly, an association of SNPs with intramuscular fat deposition has been found in pigs such as the Italian Duroc, the Danish commercial pig, as well as the Gottingen minipig 4 , 42 , 44 – 46 . In our study, we examined four SNPs, published earlier by Madsen and coworkers 42 , in 29 Polish Landrace × Duroc, Pietrain crossbreed pigs.…”

Section: Discussionmentioning

confidence: 99%

“…Genome-wide association studies (GWAS) have shown that single nucleotide polymorphisms (SNPs) in intron 1 of the FTO (Fat mass and obesity associated) gene are strongly correlated with an increased risk of obesity in humans 1 – 4 . In the human FTO gene, the mutation (alteration p. Arg316 Gln) that inhibits catalytic activity of the protein, results in an autosomal recessive lethal syndrome 5 .…”

Section: Introductionmentioning

confidence: 99%

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Intracellular and tissue specific expression of FTO protein in pig: changes with age, energy intake and metabolic status

Ferenc

Pilžys

Garbicz

et al. 2020

Sci Rep

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Genome-wide association studies in the FTO gene have identified SNPs correlating with obesity and type 2 diabetes. In mice, lack of Fto function leads to intrauterine growth retardation and lean phenotype, whereas in human it is lethal. The aim of this study in a pig model was to determine the localization of the FTO protein in different tissues and cell compartments, in order to investigate potential targets of FTO action. To better understand physiological role of FTO protein, its expression was studied in pigs of different age, metabolic status and nutrition, using both microscopic methods and Western blot analysis. For the first time, FTO protein was found in vivo in the cytoplasm, of not all, but specific tissues and cells e.g. in the pancreatic β-cells. Abundant FTO protein expression was found in the cerebellum, salivary gland and kidney of adult pigs. No FTO protein expression was detected in blood, saliva, and bile, excluding its role in cell-to-cell communication. In the pancreas, FTO protein expression was positively associated with energy intake, whereas in the muscles it was strictly agerelated. In IUGR piglets, FTO protein expression was much higher in the cerebellum and kidneys, as compared to normal birth body weight littermates. In conclusion, our data suggest that FTO protein may play a number of distinct, yet unknown intracellular functions due to its localization. Moreover, it may play a role in animal growth/development and metabolic state, although additional studies are necessary to clarify the detailed mechanism(s) of action. Genome-wide association studies (GWAS) have shown that single nucleotide polymorphisms (SNPs) in intron 1 of the FTO (Fat mass and obesity associated) gene are strongly correlated with an increased risk of obesity in humans 1-4. In the human FTO gene, the mutation (alteration p. Arg316 Gln) that inhibits catalytic activity of the protein, results in an autosomal recessive lethal syndrome 5. In Fto knockout mice, body weight and fat mass decrease 6 , while in animals overexpressing FTO they increase 7. Evidence from genetic epidemiology studies, life-course modeling, and diet-induced fetal programming data suggests that the FTO gene plays an important role in these complex biological interactions. It may provide the missing link in the developmental regulation of energy metabolism. The FTO variants associated with intrauterine growth retardation (IUGR) and, in consequence, low birth weight, confer a predisposition to obesity later in life. This finding favors the hypothesis of the existence of a common genetic denominator that predisposes to low birth weight and obesity in adults 8-10. Specifically, Fto deletion caused delayed growth, decreased white body fat, increased energy metabolism, and systemic sympathetic activation 6. For example, in wild-type mice, fasting reduced Fto mRNA levels and the number of Fto-immunoreactive cells in the hypothalamus. Interestingly, glucose treatment reversed this effect 11. However, another group of researchers showed that palatable ...

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Comparison ofMUC1variable number tandem repeat polymorphisms in three yak breeds/populations

et al. 2014

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Analysis of Yak MUC1 Protein Polymorphisms and the Corresponding VNTR Structure

Cited by 3 publications

References 16 publications

Associations between MUC1 gene polymorphism and resistance to mastitis, milk production and fertility traits in Murrah water buffaloes

Associations between MUC1 gene polymorphism and resistance to mastitis, milk production and fertility traits in Murrah water buffaloes

Intracellular and tissue specific expression of FTO protein in pig: changes with age, energy intake and metabolic status

Comparison ofMUC1variable number tandem repeat polymorphisms in three yak breeds/populations

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