Analysis of Volatile Compounds in the Pericarp of Zanthoxylum bungeanum Maxim. by Ultrasonic Nebulization Extraction Coupled with Headspace Single-Drop Microextraction and GC–MS

Wang, Lu; Wang, Ziming; Li, Xueyuan; Zhang, Huihui; Zhou, Xin; Zhang, Hanqi

doi:10.1365/s10337-010-1497-x

Cited by 29 publications

(23 citation statements)

References 13 publications

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“…The extraction efficiency of the semivolatile compounds was not so high as that of the volatile compounds. UNE-HS-SDME was used for the extraction of essential oil from plant materials in our laboratory [27,28]. The major constituents obtained by UNE-HS-SDME are basically similar to those obtained by HD.…”

Section: Introductionmentioning

confidence: 82%

Ultrasonic nebulization extraction-heating gas flow transfer-headspace single drop microextraction of essential oil from pericarp of Zanthoxylum bungeanum Maxim.

Wei

Zhang

Wang

et al. 2011

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 82%

Ultrasonic nebulization extraction-heating gas flow transfer-headspace single drop microextraction of essential oil from pericarp of Zanthoxylum bungeanum Maxim.

Wei

Zhang

Wang

et al. 2011

Journal of Chromatography A

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“…2-Dimensional NMR ( 1 H-1 H COSY, HSQC and HMBC) spectra were obtained by using standard pulse sequences. Chemical shifts were recorded in DMSO-d 6 and presented in δ (parts per million) and the coupling constants (J) in hertz.…”

Section: General Proceduresmentioning

confidence: 99%

“…The 35% aqueous methanol fraction (118.2 mg) was further purified by preparative HPLC [P270 series HPLC (Elite Analytical Instruments Co. Ltd., Dalian, China); column, Senshu Pak ODS-5251-SD 20 × 250 mm i. d. with a particle size of 5 μm (Scientific Co., Ltd., Japan); mobile phase, watermethanol-formic acid = 75: 25 : 0.1; flow rate, 16 mL/min; t R , 16.7 min; detection, UV 254 nm] to give compound 1 (7.9 mg) as yellow powders. 6.88 (2H, d, J = 7.7, H-3' and 5'), 8.00 (2H, d, J = 7.7, H-2' and 6'), 13.14 (1H, s, OH-5); 13 …”

Section: Chromatographic Separationmentioning

confidence: 99%

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Antioxidant and Cytoprotective Activities of Flavonoid Glycosides-rich Extract from the Leaves of <i>Zanthoxylum bungeanum</i>

Li¹,

Gou²,

Huang³

et al. 2014

JFNR

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The Zanthoxylum bungeanum leaf has been traditionally taken as a vegetable and seasoning in China.To disclose the mechanisms of its benefit for human health, antioxidant activity and cytoprotective effect of the leaf were investigated. The ethyl acetate fraction exhibited the strongest ABTS and DPPH radicals scavenging activities as compared with chloroform and water fractions, as well as the crude extract. This fraction exhibited a protective effect against hydrogen peroxide-induced cytotoxicity in PC12 cells by MTT reduction assay. Furthermore, the cytoprotective effect was confirmed using flow cytometry analysis. Comparing with the model cells, the ethyl acetate fraction significantly increased G0/G1 and G2/M phase cells together with reducing S phase cells. Ten flavonoid glycosides were identified as isovitexin, vitexin, hyperoside, rutin, isoquercitrin, foeniculin, trifolin, quercitrin, astragalin, and afzelin from this fraction using high performance liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance spectroscopy. This is the first report for the presence of vitexin, isovitexin, astragalin, trifolin and afzelin in Z. bungeanum. Hence, the results present here suggest the potential utility of Z. bungeanum leaf as a source of natural antioxidant due to its diversity and high content of flavonoids.

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“…The ultrasonic nebulization extraction coupled with headspace single-drop microextraction (UNE-HS-SDME) [17,18] was investigated. The experimental results indicated that UNE had good extraction efficiency.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic nebulization extraction coupled with headspace hollow fiber microextraction of pesticides from root of Panax ginseng C.A. Mey.

Wang

et al. 2011

J of Separation Science

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The ultrasonic nebulization extraction coupled with headspace hollow fiber microextraction (UNE-HS-HFME) was applied for the extraction of pesticides from root of Panax ginseng C.A. Mey. Experimental parameters, which affect the performances of ultrasonic nebulization extraction coupled with headspace hollow fiber microextraction, such as the kind of acceptor solvent in the pore of the fiber wall, the sample amount, extraction time, salt concentration in extraction solvent, pH of the acceptor solution, the elution time, and times were studied and optimized. The analytes were determined by high-performance liquid chromatography. The detection limits for simeton, monolinuron, chlortoluron, karmex, and prebane are 20.9, 18.4, 18.2, 12.4, and 22.2 μg/kg, respectively. Besides volatile and semi-volatile compounds, the non-volatile compounds also can be determined by the proposed method. The extraction and enrichment process can be performed simultaneously.

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Analysis of Volatile Compounds in the Pericarp of Zanthoxylum bungeanum Maxim. by Ultrasonic Nebulization Extraction Coupled with Headspace Single-Drop Microextraction and GC–MS

Cited by 29 publications

References 13 publications

Ultrasonic nebulization extraction-heating gas flow transfer-headspace single drop microextraction of essential oil from pericarp of Zanthoxylum bungeanum Maxim.

Ultrasonic nebulization extraction-heating gas flow transfer-headspace single drop microextraction of essential oil from pericarp of Zanthoxylum bungeanum Maxim.

Antioxidant and Cytoprotective Activities of Flavonoid Glycosides-rich Extract from the Leaves of <i>Zanthoxylum bungeanum</i>

Ultrasonic nebulization extraction coupled with headspace hollow fiber microextraction of pesticides from root of Panax ginseng C.A. Mey.

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