2001
DOI: 10.1016/s0021-9673(01)00991-8
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Analysis of vitamin E isomers in seeds and nuts with and without coupled hydrolysis by liquid chromatography and coulometric detection

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Cited by 42 publications
(29 citation statements)
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“…A. of analytical division, Lifescience facility, India. Alpha-tocopherol analysis was carried out according to Delgado-Zamarreño et al (2001) and vitamin A determined as β-carotene using AOAC Official Method 971.15 of spectrophotometric method. Water-soluble vitamins (B1, B2, B3 and folic acid) were measured with a RIDASCREEN enzyme immunoassay kit (R-Biopharm AG, Darmstadt, Germany) in accordance with the manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…A. of analytical division, Lifescience facility, India. Alpha-tocopherol analysis was carried out according to Delgado-Zamarreño et al (2001) and vitamin A determined as β-carotene using AOAC Official Method 971.15 of spectrophotometric method. Water-soluble vitamins (B1, B2, B3 and folic acid) were measured with a RIDASCREEN enzyme immunoassay kit (R-Biopharm AG, Darmstadt, Germany) in accordance with the manufacturer’s instructions.…”
Section: Methodsmentioning
confidence: 99%
“…As seeds, nuts would only be required to begin metabolism during germination of the seedling, and thus would only require low levels of these compounds (Bartz & Brecht, 2002). The most abundant vitamin in the cashew nuts was vitamin E, which serves to prevent oxidation of the unsaturated fatty acids found there (Delgado-Zamarreno, Bustamante-Rangel, Sanchez-Perez, & Hernandez-Mendez, 2001). As in other nut types, γ-tocopherol was the prevalent isomer of vitamin E found in the cashews across samples.…”
Section: Vitamins and Mineralsmentioning
confidence: 99%
“…The saponified and vitamin E isomers samples were extracted as described by Delgado-Zamarreño et al (2004) with the extraction time modified to 4 h under agitation. Fifty milliliters of ethanol, 5.0 mL of a 10% (m/v) aqueous solution of ascorbic acid, 10 mL of 80% (m/v) aqueous solution of potassium hydroxide, and 25 mL of water were added to 2.000 g of ground sample and were protected from light.…”
Section: Determination Of Tocopherol Isomers and Equivalent Vitamin Ementioning
confidence: 99%