Analysis of VH-D-JH gene transcripts in B cells infiltrating the salivary glands and lymph node tissues of patients with Sj�gren's syndrome

Abstract

These data provide evidence, on the nucleotide sequence level, that an antigen-triggered clonal B cell expansion takes place in the salivary glands of patients with SS who do not have histologic evidence of developing lymphoma. It may be speculated that those B cell clones expand during disease progression, resulting in lymphomagenesis.

Immunoglobulin variable-region gene mutational lineage tree analysis: Application to autoimmune diseases

“…A similar situation applies to the data from SS patients ( Figure 1B), where trees in one microdissection study [21] were much larger -significantly larger this time -than the trees from a second microdissection study [18]. Within the data group in [21] there were sequences extracted from two tissues, salivary gland and lymph node.…”

“…Within the data group in [21] there were sequences extracted from two tissues, salivary gland and lymph node. The salivary gland sequences were from two patients.…”

“…In others, only sequences were published [20][21][22][23]; in these cases, we identified germline genes by alignment with published human IGV [24], and trees were generated using a computer program developed by us (M. Barak et al, unpublished), which is specifically tailored to deal with IGV sequences.…”

Lineage tree analysis of immunoglobulin variable-region gene mutations in autoimmune diseases: Chronic activation, normal selection

“…In others, only sequences were published [16][17][18][19][20][21]; in these cases, germline genes were identified by alignment with published human IGV using DNAPLOT (http://vbase.mrc-cpe.cam.ac.uk/), and accordingly grouped and aligned using ClustalW (http://www.ebi.ac.uk/clustalw/). Trees were then generated using our program IgTree© (M. Barak et al, in preparation), which is specifically tailored to deal with IGV sequences.…”

“…Mutational lineage trees of B cell clones from patients with MG, RA, SS, and MS [6,[16][17][18][19][20][21], and from normal human GCs [25][26], were created from published and unpublished IgV sequence data; we also used published lineage trees [12,10,14]. Each dataset contained IGV sequences from different IGV groups and patients (Table 1).…”