Analysis of various sugars by means of immobilized enzyme coupled flow injection analysis

Weigel, B.; Hitzmann, Bernd; Kretzmer, G.; Schügerl, K.; Huwig, Alexander; Giffhorn, Friedrich

doi:10.1016/0168-1656(96)01526-x

Cited by 24 publications

(6 citation statements)

References 61 publications

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“…One disadvantage of on-line HPLC is the time delay between sampling and determination of the concentration of the observed process variable. As an alternative to on-line HPLC, the control of bioprocesses by using FIA measurements is described in [ 6 , 7 ], but there is also a time delay for the detection of the concentration of certain process variables. A frequently announced improvement of the detection of crucial process states can be achieved by using sensitive on-line software sensors in combination with mechanistic models for the estimation of process variables [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring

Faassen

Hitzmann

2015

Sensors

105

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On-line sensors for the detection of crucial process parameters are desirable for the monitoring, control and automation of processes in the biotechnology, food and pharma industry. Fluorescence spectroscopy as a highly developed and non-invasive technique that enables the on-line measurements of substrate and product concentrations or the identification of characteristic process states. During a cultivation process significant changes occur in the fluorescence spectra. By means of chemometric modeling, prediction models can be calculated and applied for process supervision and control to provide increased quality and the productivity of bioprocesses. A range of applications for different microorganisms and analytes has been proposed during the last years. This contribution provides an overview of different analysis methods for the measured fluorescence spectra and the model-building chemometric methods used for various microbial cultivations. Most of these processes are observed using the BioView® Sensor, thanks to its robustness and insensitivity to adverse process conditions. Beyond that, the PLS-method is the most frequently used chemometric method for the calculation of process models and prediction of process variables.

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Section: Introductionmentioning

confidence: 99%

Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring

Faassen

Hitzmann

2015

Sensors

105

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“…Since its first characterization by Janssen and Ruelius, POx has received attention as the key biocatalysts in various biotechnological applications; especially analytical applications in clinical chemistry, in monitoring industrial processes, in food preservation and in synthetic carbohydrate chemistry [25][26][27][28][29]. POx has also been used as a biological component in biosensor [18,19,24,27,[30][31][32][33][34][35]. Despite the large-scale industrial use of GOx, there are two main potential advantages in using POx instead of GOx.…”

Section: Introductionmentioning

confidence: 99%

Pyranose oxidase biosensor based on carbon nanotube (CNT)-modified carbon paste electrodes

Demirkol

Telefoncu

Tımur

2008

Sensors and Actuators B: Chemical

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“…Also, enzyme electrodes have been made with P2O immobilized on membranes for the detection of P2O substrates [26]. P2O from Peniophora gigantea has been used in IMERs for bioprocess monitoring [27] and from Polyporus obtusus for sugar determinations in medical applications [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

et al. 1998

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An amperometric biosensor for the detection of some monosaccharides was developed with co-immobilized pyranose oxidase (purified from the basidiomycete fungus Phanerochaete chrysosporium) and horseradish peroxidase in carbon paste. Due to rather low selectivity of pyranose oxidase, the biosensor could detect both predominating anomers of D-glucose as well as the substrates D-xylose, D-galactose, and d-gluconolactone. The best sensor performance was obtained from a carbon paste with covalently immobilized enzymes together with the additives lactitol and polyethylenimine, resulting in a sensitivity for glucose of 30.2 mA cm ¹2 mM ¹1 . The enzyme modified electrode was investigated in an automated flow injection system at the operating potential of ¹50 mV (vs. Ag/AgCl) with an injection frequency of 40 h ¹1 . The biosensor was also integrated as the detection unit in a liquid chromatographic system for the detection of monosaccharides to show its potential use in real applications.

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Analysis of various sugars by means of immobilized enzyme coupled flow injection analysis

Cited by 24 publications

References 61 publications

Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring

Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring

Pyranose oxidase biosensor based on carbon nanotube (CNT)-modified carbon paste electrodes

Pyranose Oxidase Modified Carbon Paste Electrodes for Monosaccharide Determination

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