Analysis of Various Subsets of Circulating Mononuclear Cells in Asymptomatic Coronary Artery Disease

Abstract

The objective of this study was to evaluate the correlation between multiple cardiovascular risk factors (MCRFs) and circulating mononuclear cells (CMCs) in asymptomatic coronary artery disease patients. Design and Methods: 126 subjects (54 male), aged 48 to 62 years, with asymptomatic coronary artery disease (CAD) documented previously with angiography, and 25 healthy volunteers were enrolled in the study. The flow cytometric technique was used for predictably distinguishing cell subsets that depend on the expression of CD14, CD34, Tie-2, CD45, and CD309 (VEGFR2). Results: The analysis of the outcome obtained shows a trend of an increase in circulating CD45−CD34+ CMCs and a reduction in CMC population defined as CD14+CD309+ and CD14+CD309+Tie2+ in known asymptomatic CAD patients in comparison with healthy volunteers. Substantial correlations between CD45−CD34+ and conventional cardiovascular risk factors (hs-CRP, T2DM, serum uric acid and hypertension) were found in the patient cohort. The concentrations of CD14+CD309+ and CD14+CD309+Tie2+ CMCs had effect on such factors as T2DM (RR = 1.21; 95% CI = 1.10–1.40; p = 0.008), hs-CRP > 2.54 mg/L (RR = 1.29; 95% CI = 1.12–1.58; p = 0.006), Agatston score index (RR = 1.20; 95% CI = 1.15–1.27; p = 0.034), and occurrence of three and more cardiovascular risk factors (RR = 1.31; 95% CI = 1.12–1.49; p = 0.008). Conclusion: It is postulated that the reduction in circulating CD14+CD309+ and CD14+CD309+Tei2+ CMCs is related to a number of cardiovascular risk factors in asymptomatic patients with known CAD.

Links between concentrations of serum 25-hydroxyvitamin D3 and the numbers of circulating progenitor mononuclear cells in patients with metabolic syndrome

“…From current knowledge, the depletion of proangiogenic subsets of circulating progenitor mononuclear cells is a marker of asymptomatic atherosclerosis and CV events (26,27). There is large body of evidence regarding a close inverse association between serum levels of inflammatory biomarkers (osteoprotegerin, hs-CRP, and adiponectin) and numbers/functionality of proangiogenic progenitor mononuclear cells in dysmetabolic patients with known CV disease (28).…”

Could be Used an Immune Phenotypes of Circulating Endothelial Progenitor Cells as a Biomarker of Cardiovascular Disease?

“…In population of the asymptomatic coronary artery disease patients increased circulating number of CD45-CD34+ EPCs and a reduced non-classical EPC' population defined as CD14+CD309+ and CD14+CD309+Tie2+ were found [26]. Interestingly, there was an association between number of non-classical phenotype EPCs and diabetes mellitus, highly sensitive C-reactive protein, and Agatston score index , whereas CD45-CD34+ EPCs' number did not exhibit similar relations [26].…”

“…Interestingly, there was an association between number of non-classical phenotype EPCs and diabetes mellitus, highly sensitive C-reactive protein, and Agatston score index , whereas CD45-CD34+ EPCs' number did not exhibit similar relations [26]. Moreover, incorporation of number of CD14+CD309+Tie2+ EPCs as a predictive biomarker into biomarker risk score for cumulative CV events in heart failure patients may sufficiently improve sensitivity, specificity, reliability and final discriminative value of the scale [27].…”

Serum uric acid as a metabolic regulator of endothelial function in heart failure

“…Another way that could probably explain the role of uric acid in pathogenesis of HF relates to an ability of uric acid to epigenetically regulate a survival of endothelial precursors, mediate their mobbing and differentiation, as well as coordinate a turn-over effect of metabolic memory into repair capability of cell precursors [19,20]. Indeed, SUA levels independently and inversely associated with number of circulating endothelial progenitor cells in HF individuals [21].…”