Analysis of vacA/cagA genotypes/status in Helicobacter pylori isolates from Iranian children and their association with clinical outcome

Falsafi, Tahereh; Khani, Afsaneh KhaniA.; Mahjoub, Fatemeh; Asgarani, Ezat; Sotoudeh, Nazli

doi:10.3906/sag-1311-2

Cited by 11 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning the genotypes of CagA and VacA, it was observed that the VacA s1 allele, which was the most prevalent, agreed with a prior investigation conducted on patients from Cuba; the presence of the VacA gene was observed in 61.6% of the H. pylori strains examined, with this gene being identified as the predominant virulence factor in most of the strains [ 13 ]. Nevertheless, the findings of this study opposed the previous research conducted by Kishk et al [ 22 ] [ 12 ], where they observed that the VacA+/CagA − genotype s1 had the lowest prevalence. Conversely, a separate study conducted in Algeria revealed the presence of the CagA gene in 58% of the examined patients [ 5 ].…”

Section: Discussioncontrasting

confidence: 99%

“…It was rather linked to VacA s1 in (11/55) or to Vac As2 alle in (7/55) patients, and this was consistent with [ 22 ]. Additionally, the lower percentage of the VacA s2 genotype is considered a less virulent form as compared with the acutely damaging VacA s1, as stated by Falsafi et al [ 12 ]. This study investigated the association between the VacA and CagA genotypes and clinical outcomes, as endoscopic results showed.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of concurrent infection of Helicobacter pylori with Toxoplasma gondii infection on gastric pathology

Gouda,

Saied,

Edrees

et al. 2024

BMC Infect Dis

View full text Add to dashboard Cite

Background Toxoplasma gondii (T. gondii) and Helicobacter pylori (H. pylori) are among the most prevalent foodborne parasitic and bacterial infections worldwide. However, the concurrent impact of coinfection on gastric pathology has yet to be studied in depth. The effect of coinfection generally either adds a synergetic or antagonistic impact; we aimed in the current work to assess the impact of T. gondii coinfection on the progression of H. pylori-associated gastric pathology and reporting H. pylori virulent strains. The study was conducted on 82 patients complaining of persistent gastrointestinal symptoms with failed treatment response and prone to endoscopy. They were subjected to stool examination to detect H. pylori antigen, serological screening for latent toxoplasmosis, endoscopy, histopathological examination, and molecular detection of H. pylori virulence strains in gastric biopsies. Out of the 82 patients, 62 patients were positive for H. pylori antigen in stool and 55 patients confirmed positivity by histopathology; out of them, 37 patients had isolated Vac As1 variants, 11 patients had combined Vac As1 and Cag A variants, and 7 patients had combined Vac As1, Cag A and VacAs2 variants. Patients with the combined two or three variances showed significantly deteriorated histopathological features than patients with a single Vac As1 variant (P < 0.05). Latent toxoplasmosis was positive among 35/82 patients. Combined H. pylori and Toxoplasma gondii infection had significantly marked inflammation than patients with isolated infection (P < 0.05). Conclusion: Screening for toxoplasmosis among H. pylori-infected patients is recommended as it is considered a potential risk factor for gastric inflammation severity. H. pylori gastric inflammation may be heightened by Toxoplasma coinfection.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of concurrent infection of Helicobacter pylori with Toxoplasma gondii infection on gastric pathology

Gouda,

Saied,

Edrees

et al. 2024

BMC Infect Dis

View full text Add to dashboard Cite

show abstract

“…It was rather linked to vacA s1m1 in (26/60), vacA s1m2 (5/60), or s2m2 (1/60). In this study, a lower percentage of vacA s2m2 genotype was noticed, which is considered as a less virulent form as compared with the acutely damaging vacA s1m1 as stated by Falsafi and his colleagues [28]. In our studied population, vacA+/cagA− genotype s1m1 was the least prevalent, and this is consistent with an Iranian study that was carried out in 2015 [28].…”

Section: Discussionsupporting

confidence: 91%

Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

Kishk

Soliman

Anani

et al. 2021

International Journal of Microbiology

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes’ role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.

show abstract

“…A surprisingly low prevalence of cagA was found in Portuguese children (22.4%)[21]. In Iran, the reported prevalence of cagA in symptomatic children ranges between 60.0 and 72.7%[70,71] and is similar to that in Turkish children (55.6%-61.0%)[25,72]. A high prevalence of cagA (73.0%) was also observed in symptomatic Venezuelan children with recurrent abdominal pain[73].…”

Section: Virulence Genes That Produce Toxins and Cause Host Tissue Damentioning

confidence: 99%

“…In Iranian children, nodular gastritis was commonly found and was significantly associated with the presence of vacA m1[70]. Similar to cagA , vacA s1 has been strongly associated with PUD risk (OR = 14.13, 95%CI: 4.75-42.04) among Portuguese children[42], whereas there were no significant correlations between vacA status and PUD in Iranian children[71]. Moreover, studies on Korean, Japanese and North American children found no associations between the vacA genotype and clinical outcome or severity of inflammation[75,76,88,89].…”

Section: Virulence Genes That Produce Toxins and Cause Host Tissue Damentioning

confidence: 99%

Helicobacter pylori virulence genes

Šterbenc

Jarc

Poljak

et al. 2019

WJG

114

109

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori) is one of the most important human pathogens, infecting approximately half of the global population. Despite its high prevalence, only a subset of H. pylori infected individuals develop serious gastroduodenal pathology. The pathogenesis of H. pylori infection and disease outcome is thus thought to be mediated by an intricate interplay between host, environmental and bacterial virulence factors. H. pylori has adapted to the harsh milieu of the human stomach through possession of various virulence genes that enable survival of the bacteria in the acidic environment, movement towards the gastric epithelium, and attachment to gastric epithelial cells. These virulence factors enable successful colonization of the gastric mucosa and sustain persistent H. pylori infection, causing chronic inflammation and tissue damage, which may eventually lead to the development of peptic ulcers and gastric cancer. Numerous studies have focused on the prevalence and role of putative H. pylori virulence genes in disease pathogenesis. While several virulence factors with various functions have been identified, disease associations appear to be less evident, especially among different study populations. This review presents key findings on the most important H. pylori virulence genes, including several bacterial adhesins and toxins, in children and adults, and focuses on their prevalence, clinical significance and potential relationships.

show abstract

Analysis of vacA/cagA genotypes/status in Helicobacter pylori isolates from Iranian children and their association with clinical outcome

Cited by 11 publications

References 39 publications

Effect of concurrent infection of Helicobacter pylori with Toxoplasma gondii infection on gastric pathology

Effect of concurrent infection of Helicobacter pylori with Toxoplasma gondii infection on gastric pathology

Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

Helicobacter pylori virulence genes

Contact Info

Product

Resources

About